A Label-Free Aptasensor for Ochratoxin a Detection Based on the Structure Switch of Aptamer

A Label-Free Aptasensor for Ochratoxin a Detection Based on the Structure Switch of Aptamer

A label-free sensing platform is developed based on switching the structure of aptamer for highly sensitive and selective fluorescence detection of ochratoxin A (OTA). OTA induces the structure of aptamer, transforms into G-quadruplex and produces strong fluorescence in the presence of zinc(II)-prot...

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Bibliographic Details
Main Authors: Feng Liu, Ailing Ding, Jiushang Zheng, Jiucun Chen, Bin Wang
Format: Article
Language:English
Published: MDPI AG 2018-06-01
Series:Sensors
Subjects:
aptamer
OTA
structure switching
ZnPPIX
fluorescence
Online Access:http://www.mdpi.com/1424-8220/18/6/1769
Description
Summary:A label-free sensing platform is developed based on switching the structure of aptamer for highly sensitive and selective fluorescence detection of ochratoxin A (OTA). OTA induces the structure of aptamer, transforms into G-quadruplex and produces strong fluorescence in the presence of zinc(II)-protoporphyrin IX probe due to the specific bind to G-quadruplex. The simple method exhibits high sensitivity towards OTA with a detection limit of 0.03 nM and excellent selectivity over other mycotoxins. In addition, the successful detection of OTA in real samples represents a promising application in food safety.
ISSN:1424-8220

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