The role of strigolactones in P deficiency induced transcriptional changes in tomato roots
Abstract Background Phosphorus (P) is an essential macronutrient for plant growth and development. Upon P shortage, plant responds with massive reprogramming of transcription, the Phosphate Starvation Response (PSR). In parallel, the production of strigolactones (SLs)—a class of plant hormones that...
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doaj-b6d5ef2be9e9427f93b616ce4c9a39d82021-07-25T11:07:43ZengBMCBMC Plant Biology1471-22292021-07-0121112110.1186/s12870-021-03124-0The role of strigolactones in P deficiency induced transcriptional changes in tomato rootsYanting Wang0Hernando G. Suárez Duran1Jan C. van Haarst2Elio G.W.M Schijlen3Carolien Ruyter-Spira4Marnix H. Medema5Lemeng Dong6Harro J. Bouwmeester7Plant Hormone Biology Group, Swammerdam Institute for Life Sciences, University of AmsterdamBioinformatics Group, Wageningen University and ResearchBusiness Unit Bioscience, Plant Research InternationalBusiness Unit Bioscience, Plant Research InternationalLaboratory of Plant Physiology, Wageningen University and ResearchBioinformatics Group, Wageningen University and ResearchPlant Hormone Biology Group, Swammerdam Institute for Life Sciences, University of AmsterdamPlant Hormone Biology Group, Swammerdam Institute for Life Sciences, University of AmsterdamAbstract Background Phosphorus (P) is an essential macronutrient for plant growth and development. Upon P shortage, plant responds with massive reprogramming of transcription, the Phosphate Starvation Response (PSR). In parallel, the production of strigolactones (SLs)—a class of plant hormones that regulates plant development and rhizosphere signaling molecules—increases. It is unclear, however, what the functional link is between these two processes. In this study, using tomato as a model, RNAseq was used to evaluate the time-resolved changes in gene expression in the roots upon P starvation and, using a tomato CAROTENOID CLEAVAGE DIOXYGENASES 8 (CCD8) RNAi line, what the role of SLs is in this. Results Gene ontology (GO)-term enrichment and KEGG analysis of the genes regulated by P starvation and P replenishment revealed that metabolism is an important component of the P starvation response that is aimed at P homeostasis, with large changes occurring in glyco-and galactolipid and carbohydrate metabolism, biosynthesis of secondary metabolites, including terpenoids and polyketides, glycan biosynthesis and metabolism, and amino acid metabolism. In the CCD8 RNAi line about 96% of the PSR genes was less affected than in wild-type (WT) tomato. For example, phospholipid biosynthesis was suppressed by P starvation, while the degradation of phospholipids and biosynthesis of substitute lipids such as sulfolipids and galactolipids were induced by P starvation. Around two thirds of the corresponding transcriptional changes depend on the presence of SLs. Other biosynthesis pathways are also reprogrammed under P starvation, such as phenylpropanoid and carotenoid biosynthesis, pantothenate and CoA, lysine and alkaloids, and this also partially depends on SLs. Additionally, some plant hormone biosynthetic pathways were affected by P starvation and also here, SLs are required for many of the changes (more than two thirds for Gibberellins and around one third for Abscisic acid) in the gene expression. Conclusions Our analysis shows that SLs are not just the end product of the PSR in plants (the signals secreted by plants into the rhizosphere), but also play a major role in the regulation of the PSR (as plant hormone).https://doi.org/10.1186/s12870-021-03124-0P starvationTomatoRootStrigolactoneRNAseqTranscriptional changes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yanting Wang Hernando G. Suárez Duran Jan C. van Haarst Elio G.W.M Schijlen Carolien Ruyter-Spira Marnix H. Medema Lemeng Dong Harro J. Bouwmeester |
spellingShingle |
Yanting Wang Hernando G. Suárez Duran Jan C. van Haarst Elio G.W.M Schijlen Carolien Ruyter-Spira Marnix H. Medema Lemeng Dong Harro J. Bouwmeester The role of strigolactones in P deficiency induced transcriptional changes in tomato roots BMC Plant Biology P starvation Tomato Root Strigolactone RNAseq Transcriptional changes |
author_facet |
Yanting Wang Hernando G. Suárez Duran Jan C. van Haarst Elio G.W.M Schijlen Carolien Ruyter-Spira Marnix H. Medema Lemeng Dong Harro J. Bouwmeester |
author_sort |
Yanting Wang |
title |
The role of strigolactones in P deficiency induced transcriptional changes in tomato roots |
title_short |
The role of strigolactones in P deficiency induced transcriptional changes in tomato roots |
title_full |
The role of strigolactones in P deficiency induced transcriptional changes in tomato roots |
title_fullStr |
The role of strigolactones in P deficiency induced transcriptional changes in tomato roots |
title_full_unstemmed |
The role of strigolactones in P deficiency induced transcriptional changes in tomato roots |
title_sort |
role of strigolactones in p deficiency induced transcriptional changes in tomato roots |
publisher |
BMC |
series |
BMC Plant Biology |
issn |
1471-2229 |
publishDate |
2021-07-01 |
description |
Abstract Background Phosphorus (P) is an essential macronutrient for plant growth and development. Upon P shortage, plant responds with massive reprogramming of transcription, the Phosphate Starvation Response (PSR). In parallel, the production of strigolactones (SLs)—a class of plant hormones that regulates plant development and rhizosphere signaling molecules—increases. It is unclear, however, what the functional link is between these two processes. In this study, using tomato as a model, RNAseq was used to evaluate the time-resolved changes in gene expression in the roots upon P starvation and, using a tomato CAROTENOID CLEAVAGE DIOXYGENASES 8 (CCD8) RNAi line, what the role of SLs is in this. Results Gene ontology (GO)-term enrichment and KEGG analysis of the genes regulated by P starvation and P replenishment revealed that metabolism is an important component of the P starvation response that is aimed at P homeostasis, with large changes occurring in glyco-and galactolipid and carbohydrate metabolism, biosynthesis of secondary metabolites, including terpenoids and polyketides, glycan biosynthesis and metabolism, and amino acid metabolism. In the CCD8 RNAi line about 96% of the PSR genes was less affected than in wild-type (WT) tomato. For example, phospholipid biosynthesis was suppressed by P starvation, while the degradation of phospholipids and biosynthesis of substitute lipids such as sulfolipids and galactolipids were induced by P starvation. Around two thirds of the corresponding transcriptional changes depend on the presence of SLs. Other biosynthesis pathways are also reprogrammed under P starvation, such as phenylpropanoid and carotenoid biosynthesis, pantothenate and CoA, lysine and alkaloids, and this also partially depends on SLs. Additionally, some plant hormone biosynthetic pathways were affected by P starvation and also here, SLs are required for many of the changes (more than two thirds for Gibberellins and around one third for Abscisic acid) in the gene expression. Conclusions Our analysis shows that SLs are not just the end product of the PSR in plants (the signals secreted by plants into the rhizosphere), but also play a major role in the regulation of the PSR (as plant hormone). |
topic |
P starvation Tomato Root Strigolactone RNAseq Transcriptional changes |
url |
https://doi.org/10.1186/s12870-021-03124-0 |
work_keys_str_mv |
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