Distribution of BoLA-DRB3 Allelic Frequencies and Identification of Two New Alleles in Iranian Buffalo Breed
The role of the major histocompatibility complex (MHC) in the immune response makes it an attractive candidate gene for associations with disease resistance and susceptibility. This study describes genetic variability in the BoLA-DRB3 in Iranian buffaloes. Heminested PCR-RFLP method was used to iden...
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doaj-b6deba51d2d8413e9428115ec385bba22020-11-25T02:11:49ZengHindawi LimitedThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/863024863024Distribution of BoLA-DRB3 Allelic Frequencies and Identification of Two New Alleles in Iranian Buffalo BreedJ. Mosafer0M. Heydarpour1E. Manshad2G. Russell3G. E. Sulimova4Department of Animal Science, Ferdowsi University of Mashhad, P.O. Box 91775-1163, 9177948974 Mashhad, IranPopulation Health Research Institute (PHRI), Department of Medicine, McMaster University, Hamilton, ON, L8L 2X2, CanadaDepartment of Animal Science, Ferdowsi University of Mashhad, P.O. Box 91775-1163, 9177948974 Mashhad, IranMoredun Research Institute, Pentlands Science Park, Midlothian, Penicuik EH26 0PZ, UKVavilov Institute of General Genetics, Russian Academy of Sciences, Moscow 119991, RussiaThe role of the major histocompatibility complex (MHC) in the immune response makes it an attractive candidate gene for associations with disease resistance and susceptibility. This study describes genetic variability in the BoLA-DRB3 in Iranian buffaloes. Heminested PCR-RFLP method was used to identify the frequency of BoLA-DRB3 alleles. The BoLA-DRB3 locus is highly polymorphic in the study herd (12 alleles). Almost 63.50% of the alleles were accounted for by four alleles (BoLA-DRB3.2 *48, *20, *21, and obe) in Iranian buffalo. The DRB3.2 *48 allele frequency (24.20%) was higher than the others. The frequencies of the DRB3.2 *20 and DRB3.2 *21 are 14.52 and 14.00, respectively, and obe and gbb have a new pattern. Significant distinctions have been found between Iranian buffalo and other cattle breed studied. In the Iranian buffaloes studied alleles associated with resistance to various diseases are found.http://dx.doi.org/10.1100/2012/863024 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
J. Mosafer M. Heydarpour E. Manshad G. Russell G. E. Sulimova |
spellingShingle |
J. Mosafer M. Heydarpour E. Manshad G. Russell G. E. Sulimova Distribution of BoLA-DRB3 Allelic Frequencies and Identification of Two New Alleles in Iranian Buffalo Breed The Scientific World Journal |
author_facet |
J. Mosafer M. Heydarpour E. Manshad G. Russell G. E. Sulimova |
author_sort |
J. Mosafer |
title |
Distribution of BoLA-DRB3 Allelic Frequencies and Identification of Two New Alleles in Iranian Buffalo Breed |
title_short |
Distribution of BoLA-DRB3 Allelic Frequencies and Identification of Two New Alleles in Iranian Buffalo Breed |
title_full |
Distribution of BoLA-DRB3 Allelic Frequencies and Identification of Two New Alleles in Iranian Buffalo Breed |
title_fullStr |
Distribution of BoLA-DRB3 Allelic Frequencies and Identification of Two New Alleles in Iranian Buffalo Breed |
title_full_unstemmed |
Distribution of BoLA-DRB3 Allelic Frequencies and Identification of Two New Alleles in Iranian Buffalo Breed |
title_sort |
distribution of bola-drb3 allelic frequencies and identification of two new alleles in iranian buffalo breed |
publisher |
Hindawi Limited |
series |
The Scientific World Journal |
issn |
1537-744X |
publishDate |
2012-01-01 |
description |
The role of the major histocompatibility complex (MHC) in the immune response makes it an attractive candidate gene for associations with disease resistance and susceptibility. This study describes genetic variability in the BoLA-DRB3 in Iranian buffaloes. Heminested PCR-RFLP method was used to identify the frequency of BoLA-DRB3 alleles. The BoLA-DRB3 locus is highly polymorphic in the study herd (12 alleles). Almost 63.50% of the alleles were accounted for by four alleles (BoLA-DRB3.2 *48, *20, *21, and obe) in Iranian buffalo. The DRB3.2 *48 allele frequency (24.20%) was higher than the others. The frequencies of the DRB3.2 *20 and DRB3.2 *21 are 14.52 and 14.00, respectively, and obe and gbb have a new pattern. Significant distinctions have been found between Iranian buffalo and other cattle breed studied. In the Iranian buffaloes studied alleles associated with resistance to various diseases are found. |
url |
http://dx.doi.org/10.1100/2012/863024 |
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