Stable and quantitative small-scale laboratory propagation of Cryptocaryon irritans
We established a laboratory propagation method of Cryptocaryon irritans, a parasitic ciliate of marine fishes, with black molly Poecilia sp. as host fish, using small plastic aquaria. One cycle of the propagation usually takes one week. With this method, 1500–3000 protomonts are obtained from five c...
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doaj-b6ea8cd6ef3f42f1ada724a9b0c90ed02021-01-02T05:10:45ZengElsevierMethodsX2215-01612020-01-017101000Stable and quantitative small-scale laboratory propagation of Cryptocaryon irritansYuho Watanabe0Kah Hui How1Kosuke Zenke2Naoki Itoh3Tomoyoshi Yoshinaga4Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657, JapanDepartment of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657, JapanDepartment of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657, JapanDepartment of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657, JapanCorresponding author.; Department of Aquatic Bioscience, Graduate School of Agriculture and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657, JapanWe established a laboratory propagation method of Cryptocaryon irritans, a parasitic ciliate of marine fishes, with black molly Poecilia sp. as host fish, using small plastic aquaria. One cycle of the propagation usually takes one week. With this method, 1500–3000 protomonts are obtained from five challenged mollies every week, from which more than 100,000–200,000 theronts are obtained. Using this method, an isolate of C. irritans has been successfully maintained more than three years. This propagation method reduces labor for maintaining and propagating the parasite and will much contribute to researches on cryptocaryoniasis. • The method is a laboratory propagation technique of Cryptocaryon irritans. • Using small plastic aquaria and black molly as a host, the parasites can be stably propagated and maintained. • An isolate of C. irritans has been successfully maintained more than three years.http://www.sciencedirect.com/science/article/pii/S221501612030220XIn vivoControlled challengePoecilia spInfection |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuho Watanabe Kah Hui How Kosuke Zenke Naoki Itoh Tomoyoshi Yoshinaga |
spellingShingle |
Yuho Watanabe Kah Hui How Kosuke Zenke Naoki Itoh Tomoyoshi Yoshinaga Stable and quantitative small-scale laboratory propagation of Cryptocaryon irritans MethodsX In vivo Controlled challenge Poecilia sp Infection |
author_facet |
Yuho Watanabe Kah Hui How Kosuke Zenke Naoki Itoh Tomoyoshi Yoshinaga |
author_sort |
Yuho Watanabe |
title |
Stable and quantitative small-scale laboratory propagation of Cryptocaryon irritans |
title_short |
Stable and quantitative small-scale laboratory propagation of Cryptocaryon irritans |
title_full |
Stable and quantitative small-scale laboratory propagation of Cryptocaryon irritans |
title_fullStr |
Stable and quantitative small-scale laboratory propagation of Cryptocaryon irritans |
title_full_unstemmed |
Stable and quantitative small-scale laboratory propagation of Cryptocaryon irritans |
title_sort |
stable and quantitative small-scale laboratory propagation of cryptocaryon irritans |
publisher |
Elsevier |
series |
MethodsX |
issn |
2215-0161 |
publishDate |
2020-01-01 |
description |
We established a laboratory propagation method of Cryptocaryon irritans, a parasitic ciliate of marine fishes, with black molly Poecilia sp. as host fish, using small plastic aquaria. One cycle of the propagation usually takes one week. With this method, 1500–3000 protomonts are obtained from five challenged mollies every week, from which more than 100,000–200,000 theronts are obtained. Using this method, an isolate of C. irritans has been successfully maintained more than three years. This propagation method reduces labor for maintaining and propagating the parasite and will much contribute to researches on cryptocaryoniasis. • The method is a laboratory propagation technique of Cryptocaryon irritans. • Using small plastic aquaria and black molly as a host, the parasites can be stably propagated and maintained. • An isolate of C. irritans has been successfully maintained more than three years. |
topic |
In vivo Controlled challenge Poecilia sp Infection |
url |
http://www.sciencedirect.com/science/article/pii/S221501612030220X |
work_keys_str_mv |
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