Protective Effect of Aerobic Training along with Resveratrol on Mitochondrial Dynamics of Cardiac Myocytes in Animal Model of Non-alcoholic Fatty Liver Disease
Background & objectives: Non-alcoholic fatty liver disease (NAFLD) is associated with mitochondrial dysfunction. The aim of the present study was to examine the effect of aerobic training along with resveratrol on cardiac expression of OPA1 and DRP1 in NAFLD male rats. Methods: In this experimen...
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doaj-b6f157547979418285f126924316d4c42020-11-25T03:10:23ZfasArdabil University of Medical SciencesJournal of Ardabil University of Medical Sciences2228-72802228-72992019-10-01193272283Protective Effect of Aerobic Training along with Resveratrol on Mitochondrial Dynamics of Cardiac Myocytes in Animal Model of Non-alcoholic Fatty Liver Diseasehasan delroz0Ahmad Abdi1Alireza Barari2Parvin Farzanegi3 Department of Exercise Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran Department of Exercise Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran Department of Exercise Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran Department of Sport Physiology, Sari Branch, Islamic Azad University, Sari, Iran Background & objectives: Non-alcoholic fatty liver disease (NAFLD) is associated with mitochondrial dysfunction. The aim of the present study was to examine the effect of aerobic training along with resveratrol on cardiac expression of OPA1 and DRP1 in NAFLD male rats. Methods: In this experimental study, forty eight male Wistar rats were classified into two groups: NAFLD (n=40) and Control-Normal (CN), (n=8). NAFLD was induced in rats with high fat diet and then subdivided into five subgroups, including patient (NAFLD), sham (SHAM), exercise-patient (TRNAF), supplement-patient (SUPNAF) and exercise-supplement-patient (TRSUPNAF). Training groups performed a running program on a motor-driven treadmill for eight weeks. Resveratrol (20 mg/kg) was injected into the SUPNAF and TRSUPNAF groups. Forty eight hours after the last training session, rats were anesthetized; hearts were excised and immediately frozen in liquid nitrogen and stored at -80°C for measuring the indexes. Statistical analysis was performed using a one way analysis of variance, and Statistical significance was accepted at p≤ 0.05. Result: The results indicated that the induction of NAFLD decreased OPA1 expression (p=0.001) and increased DRP1 expression (p=0.001) compared to CN. Also, in SUPNAFLD and TRSUPNAF groups, OPA1 (p=0.014 and p=0.001 respectively) increased and DRP1 (p=0.043 and p=0.007 respectively) decreased significantly compared to the NAFLD group. Conclusion: It seems that exercise training and resveratrol alone or in combination by increasing OPA1 and decreasing DRP1 myocytes modulates heart damage in NAFLD patients.http://jarums.arums.ac.ir/article-1-1742-en.htmlaerobic exerciseresveratrolopa1drp1fatty liver |
collection |
DOAJ |
language |
fas |
format |
Article |
sources |
DOAJ |
author |
hasan delroz Ahmad Abdi Alireza Barari Parvin Farzanegi |
spellingShingle |
hasan delroz Ahmad Abdi Alireza Barari Parvin Farzanegi Protective Effect of Aerobic Training along with Resveratrol on Mitochondrial Dynamics of Cardiac Myocytes in Animal Model of Non-alcoholic Fatty Liver Disease Journal of Ardabil University of Medical Sciences aerobic exercise resveratrol opa1 drp1 fatty liver |
author_facet |
hasan delroz Ahmad Abdi Alireza Barari Parvin Farzanegi |
author_sort |
hasan delroz |
title |
Protective Effect of Aerobic Training along with Resveratrol on Mitochondrial Dynamics of Cardiac Myocytes in Animal Model of Non-alcoholic Fatty Liver Disease |
title_short |
Protective Effect of Aerobic Training along with Resveratrol on Mitochondrial Dynamics of Cardiac Myocytes in Animal Model of Non-alcoholic Fatty Liver Disease |
title_full |
Protective Effect of Aerobic Training along with Resveratrol on Mitochondrial Dynamics of Cardiac Myocytes in Animal Model of Non-alcoholic Fatty Liver Disease |
title_fullStr |
Protective Effect of Aerobic Training along with Resveratrol on Mitochondrial Dynamics of Cardiac Myocytes in Animal Model of Non-alcoholic Fatty Liver Disease |
title_full_unstemmed |
Protective Effect of Aerobic Training along with Resveratrol on Mitochondrial Dynamics of Cardiac Myocytes in Animal Model of Non-alcoholic Fatty Liver Disease |
title_sort |
protective effect of aerobic training along with resveratrol on mitochondrial dynamics of cardiac myocytes in animal model of non-alcoholic fatty liver disease |
publisher |
Ardabil University of Medical Sciences |
series |
Journal of Ardabil University of Medical Sciences |
issn |
2228-7280 2228-7299 |
publishDate |
2019-10-01 |
description |
Background & objectives: Non-alcoholic fatty liver disease (NAFLD) is associated with mitochondrial dysfunction. The aim of the present study was to examine the effect of aerobic training along with resveratrol on cardiac expression of OPA1 and DRP1 in NAFLD male rats.
Methods: In this experimental study, forty eight male Wistar rats were classified into two groups: NAFLD (n=40) and Control-Normal (CN), (n=8). NAFLD was induced in rats with high fat diet and then subdivided into five subgroups, including patient (NAFLD), sham (SHAM), exercise-patient (TRNAF), supplement-patient (SUPNAF) and exercise-supplement-patient (TRSUPNAF). Training groups performed a running program on a motor-driven treadmill for eight weeks. Resveratrol (20 mg/kg) was injected into the SUPNAF and TRSUPNAF groups. Forty eight hours after the last training session, rats were anesthetized; hearts were excised and immediately frozen in liquid nitrogen and stored at -80°C for measuring the indexes. Statistical analysis was performed using a one way analysis of variance, and Statistical significance was accepted at p≤ 0.05.
Result: The results indicated that the induction of NAFLD decreased OPA1 expression (p=0.001) and increased DRP1 expression (p=0.001) compared to CN. Also, in SUPNAFLD and TRSUPNAF groups, OPA1 (p=0.014 and p=0.001 respectively) increased and DRP1 (p=0.043 and p=0.007 respectively) decreased significantly compared to the NAFLD group.
Conclusion: It seems that exercise training and resveratrol alone or in combination by increasing OPA1 and decreasing DRP1 myocytes modulates heart damage in NAFLD patients. |
topic |
aerobic exercise resveratrol opa1 drp1 fatty liver |
url |
http://jarums.arums.ac.ir/article-1-1742-en.html |
work_keys_str_mv |
AT hasandelroz protectiveeffectofaerobictrainingalongwithresveratrolonmitochondrialdynamicsofcardiacmyocytesinanimalmodelofnonalcoholicfattyliverdisease AT ahmadabdi protectiveeffectofaerobictrainingalongwithresveratrolonmitochondrialdynamicsofcardiacmyocytesinanimalmodelofnonalcoholicfattyliverdisease AT alirezabarari protectiveeffectofaerobictrainingalongwithresveratrolonmitochondrialdynamicsofcardiacmyocytesinanimalmodelofnonalcoholicfattyliverdisease AT parvinfarzanegi protectiveeffectofaerobictrainingalongwithresveratrolonmitochondrialdynamicsofcardiacmyocytesinanimalmodelofnonalcoholicfattyliverdisease |
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