Transcriptomic responses of Serratia liquefaciens cells grown under simulated Martian conditions of low temperature, low pressure, and CO2-enriched anoxic atmosphere

Abstract Results from previous experiments indicated that the Gram-negative α-proteobacterium Serratia liquefaciens strain ATCC 27592 was capable of growth under low temperature (0 °C), low pressure (0.7 kPa), and anoxic, CO2-dominated atmosphere–conditions intended to simulate the near-subsurface e...

Full description

Bibliographic Details
Main Authors: Patricia Fajardo-Cavazos, Michael D. Morrison, Kathleen M. Miller, Andrew C. Schuerger, Wayne L. Nicholson
Format: Article
Language:English
Published: Nature Publishing Group 2018-10-01
Series:Scientific Reports
Subjects:
Online Access:https://doi.org/10.1038/s41598-018-33140-4
id doaj-b6fdb3d77da74d8ea5c3f677a668f857
record_format Article
spelling doaj-b6fdb3d77da74d8ea5c3f677a668f8572020-12-08T05:45:31ZengNature Publishing GroupScientific Reports2045-23222018-10-018111010.1038/s41598-018-33140-4Transcriptomic responses of Serratia liquefaciens cells grown under simulated Martian conditions of low temperature, low pressure, and CO2-enriched anoxic atmospherePatricia Fajardo-Cavazos0Michael D. Morrison1Kathleen M. Miller2Andrew C. Schuerger3Wayne L. Nicholson4Department of Microbiology and Cell Science, University of FloridaDepartment of Microbiology and Cell Science, University of FloridaDepartment of Microbiology and Cell Science, University of FloridaDepartment of Plant Pathology, University of FloridaDepartment of Microbiology and Cell Science, University of FloridaAbstract Results from previous experiments indicated that the Gram-negative α-proteobacterium Serratia liquefaciens strain ATCC 27592 was capable of growth under low temperature (0 °C), low pressure (0.7 kPa), and anoxic, CO2-dominated atmosphere–conditions intended to simulate the near-subsurface environment of Mars. To probe the response of its transcriptome to this extreme environment, S. liquefaciens ATCC 27592 was cultivated under 4 different environmental simulations: 0 °C, 0.7 kPa, CO2 atmosphere (Condition A); 0 °C, ~101.3 kPa, CO2 atmosphere (Condition B); 0 °C, ~101.3 kPa, ambient N2/O2 atmosphere (Condition C); and 30 °C, ~101.3 kPa, N2/O2 atmosphere (Condition D; ambient laboratory conditions). RNA-seq was performed on ribosomal RNA-depleted total RNA isolated from triplicate cultures grown under Conditions A-D and the datasets generated were subjected to transcriptome analyses. The data from Conditions A, B, or C were compared to laboratory Condition D. Significantly differentially expressed transcripts were identified belonging to a number of KEGG pathway categories. Up-regulated genes under all Conditions A, B, and C included those encoding transporters (ABC and PTS transporters); genes involved in translation (ribosomes and their biogenesis, biosynthesis of both tRNAs and aminoacyl-tRNAs); DNA repair and recombination; and non-coding RNAs. Genes down-regulated under all Conditions A, B, and C included: transporters (mostly ABC transporters); flagellar and motility proteins; genes involved in phenylalanine metabolism; transcription factors; and two-component systems. The results are discussed in the context of Mars astrobiology and planetary protection.https://doi.org/10.1038/s41598-018-33140-4Simulated Mars ConditionsTranscriptomic ResponseKyoto Encyclopedia Of Genes And Genomes (KEGG)KEGG Pathway CategoriesPlanetary Protection
collection DOAJ
language English
format Article
sources DOAJ
author Patricia Fajardo-Cavazos
Michael D. Morrison
Kathleen M. Miller
Andrew C. Schuerger
Wayne L. Nicholson
spellingShingle Patricia Fajardo-Cavazos
Michael D. Morrison
Kathleen M. Miller
Andrew C. Schuerger
Wayne L. Nicholson
Transcriptomic responses of Serratia liquefaciens cells grown under simulated Martian conditions of low temperature, low pressure, and CO2-enriched anoxic atmosphere
Scientific Reports
Simulated Mars Conditions
Transcriptomic Response
Kyoto Encyclopedia Of Genes And Genomes (KEGG)
KEGG Pathway Categories
Planetary Protection
author_facet Patricia Fajardo-Cavazos
Michael D. Morrison
Kathleen M. Miller
Andrew C. Schuerger
Wayne L. Nicholson
author_sort Patricia Fajardo-Cavazos
title Transcriptomic responses of Serratia liquefaciens cells grown under simulated Martian conditions of low temperature, low pressure, and CO2-enriched anoxic atmosphere
title_short Transcriptomic responses of Serratia liquefaciens cells grown under simulated Martian conditions of low temperature, low pressure, and CO2-enriched anoxic atmosphere
title_full Transcriptomic responses of Serratia liquefaciens cells grown under simulated Martian conditions of low temperature, low pressure, and CO2-enriched anoxic atmosphere
title_fullStr Transcriptomic responses of Serratia liquefaciens cells grown under simulated Martian conditions of low temperature, low pressure, and CO2-enriched anoxic atmosphere
title_full_unstemmed Transcriptomic responses of Serratia liquefaciens cells grown under simulated Martian conditions of low temperature, low pressure, and CO2-enriched anoxic atmosphere
title_sort transcriptomic responses of serratia liquefaciens cells grown under simulated martian conditions of low temperature, low pressure, and co2-enriched anoxic atmosphere
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2018-10-01
description Abstract Results from previous experiments indicated that the Gram-negative α-proteobacterium Serratia liquefaciens strain ATCC 27592 was capable of growth under low temperature (0 °C), low pressure (0.7 kPa), and anoxic, CO2-dominated atmosphere–conditions intended to simulate the near-subsurface environment of Mars. To probe the response of its transcriptome to this extreme environment, S. liquefaciens ATCC 27592 was cultivated under 4 different environmental simulations: 0 °C, 0.7 kPa, CO2 atmosphere (Condition A); 0 °C, ~101.3 kPa, CO2 atmosphere (Condition B); 0 °C, ~101.3 kPa, ambient N2/O2 atmosphere (Condition C); and 30 °C, ~101.3 kPa, N2/O2 atmosphere (Condition D; ambient laboratory conditions). RNA-seq was performed on ribosomal RNA-depleted total RNA isolated from triplicate cultures grown under Conditions A-D and the datasets generated were subjected to transcriptome analyses. The data from Conditions A, B, or C were compared to laboratory Condition D. Significantly differentially expressed transcripts were identified belonging to a number of KEGG pathway categories. Up-regulated genes under all Conditions A, B, and C included those encoding transporters (ABC and PTS transporters); genes involved in translation (ribosomes and their biogenesis, biosynthesis of both tRNAs and aminoacyl-tRNAs); DNA repair and recombination; and non-coding RNAs. Genes down-regulated under all Conditions A, B, and C included: transporters (mostly ABC transporters); flagellar and motility proteins; genes involved in phenylalanine metabolism; transcription factors; and two-component systems. The results are discussed in the context of Mars astrobiology and planetary protection.
topic Simulated Mars Conditions
Transcriptomic Response
Kyoto Encyclopedia Of Genes And Genomes (KEGG)
KEGG Pathway Categories
Planetary Protection
url https://doi.org/10.1038/s41598-018-33140-4
work_keys_str_mv AT patriciafajardocavazos transcriptomicresponsesofserratialiquefacienscellsgrownundersimulatedmartianconditionsoflowtemperaturelowpressureandco2enrichedanoxicatmosphere
AT michaeldmorrison transcriptomicresponsesofserratialiquefacienscellsgrownundersimulatedmartianconditionsoflowtemperaturelowpressureandco2enrichedanoxicatmosphere
AT kathleenmmiller transcriptomicresponsesofserratialiquefacienscellsgrownundersimulatedmartianconditionsoflowtemperaturelowpressureandco2enrichedanoxicatmosphere
AT andrewcschuerger transcriptomicresponsesofserratialiquefacienscellsgrownundersimulatedmartianconditionsoflowtemperaturelowpressureandco2enrichedanoxicatmosphere
AT waynelnicholson transcriptomicresponsesofserratialiquefacienscellsgrownundersimulatedmartianconditionsoflowtemperaturelowpressureandco2enrichedanoxicatmosphere
_version_ 1724391680123076608