Molecular characterization of transcriptional regulation of rovA by PhoP and RovA in Yersinia pestis.
BACKGROUND: Yersinia pestis is the causative agent of plague. The two transcriptional regulators, PhoP and RovA, are required for the virulence of Y. pestis through the regulation of various virulence-associated loci. They are the global regulators controlling two distinct large complexes of cellula...
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doaj-b70714aa6ff14667ac4865a276c6e6d82020-11-25T01:42:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0169e2548410.1371/journal.pone.0025484Molecular characterization of transcriptional regulation of rovA by PhoP and RovA in Yersinia pestis.Yiquan ZhangHe GaoLi WangXiao XiaoYafang TanZhaobiao GuoDongsheng ZhouRuifu YangBACKGROUND: Yersinia pestis is the causative agent of plague. The two transcriptional regulators, PhoP and RovA, are required for the virulence of Y. pestis through the regulation of various virulence-associated loci. They are the global regulators controlling two distinct large complexes of cellular pathways. METHODOLOGY/PRINCIPAL FINDINGS: Based on the LacZ fusion, primer extension, gel mobility shift, and DNase I footprinting assays, RovA is shown to recognize both of the two promoters of its gene in Y. pestis. The autoregulation of RovA appears to be a conserved mechanism shared by Y. pestis and its closely related progenitor, Y. pseudotuberculosis. In Y. pestis, the PhoP regulator responds to low magnesium signals and then negatively controls only one of the two promoters of rovA through PhoP-promoter DNA association. CONCLUSIONS/SIGNIFICANCE: RovA is a direct transcriptional activator for its own gene in Y. pestis, while PhoP recognizes the promoter region of rovA to repress its transcription. The direct regulatory association between PhoP and RovA bridges the PhoP and RovA regulons in Y. pestis.http://europepmc.org/articles/PMC3180457?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yiquan Zhang He Gao Li Wang Xiao Xiao Yafang Tan Zhaobiao Guo Dongsheng Zhou Ruifu Yang |
spellingShingle |
Yiquan Zhang He Gao Li Wang Xiao Xiao Yafang Tan Zhaobiao Guo Dongsheng Zhou Ruifu Yang Molecular characterization of transcriptional regulation of rovA by PhoP and RovA in Yersinia pestis. PLoS ONE |
author_facet |
Yiquan Zhang He Gao Li Wang Xiao Xiao Yafang Tan Zhaobiao Guo Dongsheng Zhou Ruifu Yang |
author_sort |
Yiquan Zhang |
title |
Molecular characterization of transcriptional regulation of rovA by PhoP and RovA in Yersinia pestis. |
title_short |
Molecular characterization of transcriptional regulation of rovA by PhoP and RovA in Yersinia pestis. |
title_full |
Molecular characterization of transcriptional regulation of rovA by PhoP and RovA in Yersinia pestis. |
title_fullStr |
Molecular characterization of transcriptional regulation of rovA by PhoP and RovA in Yersinia pestis. |
title_full_unstemmed |
Molecular characterization of transcriptional regulation of rovA by PhoP and RovA in Yersinia pestis. |
title_sort |
molecular characterization of transcriptional regulation of rova by phop and rova in yersinia pestis. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2011-01-01 |
description |
BACKGROUND: Yersinia pestis is the causative agent of plague. The two transcriptional regulators, PhoP and RovA, are required for the virulence of Y. pestis through the regulation of various virulence-associated loci. They are the global regulators controlling two distinct large complexes of cellular pathways. METHODOLOGY/PRINCIPAL FINDINGS: Based on the LacZ fusion, primer extension, gel mobility shift, and DNase I footprinting assays, RovA is shown to recognize both of the two promoters of its gene in Y. pestis. The autoregulation of RovA appears to be a conserved mechanism shared by Y. pestis and its closely related progenitor, Y. pseudotuberculosis. In Y. pestis, the PhoP regulator responds to low magnesium signals and then negatively controls only one of the two promoters of rovA through PhoP-promoter DNA association. CONCLUSIONS/SIGNIFICANCE: RovA is a direct transcriptional activator for its own gene in Y. pestis, while PhoP recognizes the promoter region of rovA to repress its transcription. The direct regulatory association between PhoP and RovA bridges the PhoP and RovA regulons in Y. pestis. |
url |
http://europepmc.org/articles/PMC3180457?pdf=render |
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