Investigation of a Medical Plant for Hepatic Diseases with Secoiridoids Using HPLC and FT-IR Spectroscopy for a Case of <i>Gentiana rigescens</i>

• Main Authors: Yuangui Yang, Yanli Zhao, Zhitian Zuo, Ji Zhang, Yao Shi, Yuanzhong Wang
• Format: Article
• Language: English
• Published: MDPI AG 2020-03-01
• Series: Molecules
• Subjects: hepatic diseases; secoiridoids; <i>g. rigenscens</i>; hplc; ft-ir; plsr
• Online Access: https://www.mdpi.com/1420-3049/25/5/1219

Secoiridoids could be used as a potential new drug for the treatment of hepatic disease. The content of secoiridoids of <i>G. rigescens</i> varied in different geographical origins and parts. In this study, a total of 783 samples collected from different parts of <i>G. rigescens</i> in Yunnan, Sichuan, and Guizhou Provinces. The content of secoiridoids including gentiopicroside, swertiamarin, and sweroside were determined by using HPLC and analyzed by one-way analysis of variance. Two selected variables including direct selected and variable importance in projection combined with partial least squares regression have been used to establish a method for the determination of secoiridoids using FT-IR spectroscopy. In addition, different pretreatments including multiplicative scatter correction (MSC), standard normal variate (SNV), first derivative and second derivative (SD), and orthogonal signal correction (OSC) were compared. The results indicated that the sample (root, stem, and leaf) with total secoiridoids, gentiopicroside, swertiamarin, and sweroside from west Yunnan had higher content than samples from the other regions. The sample from Baoshan had more total secoiridoids than other samples for the whole medicinal plant. The best performance using FT-IR for the total secoiridoid was with the direct selected variable method involving pretreatment of MSC+OSC+SD in the root and stem, while in leaf, of the best method involved using original data with MSC+OSC+SD. This method could be used to determine the bioactive compounds quickly for herbal medicines.