First Detection of Nosema ceranae, a Microsporidian Protozoa of European Honeybees (Apis mellifera) In Iran
Background: Nosemosis of European honey bee (Apis mellifera) is present in bee colonies worldwide. Until recently, Nosema apis had been regarded as the causative agent of the disease, that causes heavy economic losses in apicultures. Nosema ceranae is an emerging microsporidian parasite of Europea...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Tehran University of Medical Sciences
2011-09-01
|
Series: | Iranian Journal of Parasitology |
Subjects: | |
Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/193 |
id |
doaj-b713e9156ca7414eb3d10555d85d553e |
---|---|
record_format |
Article |
spelling |
doaj-b713e9156ca7414eb3d10555d85d553e2021-04-02T14:05:22ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2011-09-0163First Detection of Nosema ceranae, a Microsporidian Protozoa of European Honeybees (Apis mellifera) In IranS Nabian0K Ahmadi1MH Nazem Shirazi2A Gerami Sadeghian3Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, IranVeterinary Organization of Iran, Tehran, IranVeterinary Organization of Iran, Tehran, IranDepartment of Parasitology, Faculty of Veterinary Medicine, University of Tehran, IranBackground: Nosemosis of European honey bee (Apis mellifera) is present in bee colonies worldwide. Until recently, Nosema apis had been regarded as the causative agent of the disease, that causes heavy economic losses in apicultures. Nosema ceranae is an emerging microsporidian parasite of European honeybees, A. mellifera, but its distribution is not well known. Previously, nosemosis in honeybees in Iran was attributed exclusively to N. apis. Methods: Six Nosema positive samples (determined from light microscopy of spores) of adult worker bees from one province of Iran (Savadkouh- Mazandaran, northern Iran) were tested to determine Nosema species using previously- developed PCR primers of the 16 S rRNA gene. As it is difficult to distinguish N. ceranae and N. apis morphologically, a PCR assay based on 16 S ribosomal RNA has been used to differentiate N. apis and N. ceranae. Results: Only N. ceranae was found in all samples, indicating that this species present in Iran apiaries. Conclusion: This is the first report of N. ceranae in colonies of A. mellifera in Iran. It seems that intensive surveys are needed to determine the distribution and prevalence of N. ceranae in different regions of Iran. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/193HoneybeeNosema apisNosema ceranaePCR |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
S Nabian K Ahmadi MH Nazem Shirazi A Gerami Sadeghian |
spellingShingle |
S Nabian K Ahmadi MH Nazem Shirazi A Gerami Sadeghian First Detection of Nosema ceranae, a Microsporidian Protozoa of European Honeybees (Apis mellifera) In Iran Iranian Journal of Parasitology Honeybee Nosema apis Nosema ceranae PCR |
author_facet |
S Nabian K Ahmadi MH Nazem Shirazi A Gerami Sadeghian |
author_sort |
S Nabian |
title |
First Detection of Nosema ceranae, a Microsporidian Protozoa of European Honeybees (Apis mellifera) In Iran |
title_short |
First Detection of Nosema ceranae, a Microsporidian Protozoa of European Honeybees (Apis mellifera) In Iran |
title_full |
First Detection of Nosema ceranae, a Microsporidian Protozoa of European Honeybees (Apis mellifera) In Iran |
title_fullStr |
First Detection of Nosema ceranae, a Microsporidian Protozoa of European Honeybees (Apis mellifera) In Iran |
title_full_unstemmed |
First Detection of Nosema ceranae, a Microsporidian Protozoa of European Honeybees (Apis mellifera) In Iran |
title_sort |
first detection of nosema ceranae, a microsporidian protozoa of european honeybees (apis mellifera) in iran |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Parasitology |
issn |
1735-7020 2008-238X |
publishDate |
2011-09-01 |
description |
Background: Nosemosis of European honey bee (Apis mellifera) is present in bee colonies worldwide. Until recently, Nosema apis had been regarded as the causative agent of the disease, that causes heavy economic losses in apicultures. Nosema ceranae is an emerging microsporidian parasite of European honeybees, A. mellifera, but its distribution is not well known. Previously, nosemosis in honeybees in Iran was attributed exclusively to N. apis.
Methods: Six Nosema positive samples (determined from light microscopy of spores) of adult worker bees from one province of Iran (Savadkouh- Mazandaran, northern Iran) were tested to determine Nosema species using previously- developed PCR primers of the 16 S rRNA gene. As it is difficult to distinguish N. ceranae and N. apis morphologically, a PCR assay based on 16 S ribosomal RNA has been used to differentiate N. apis and N. ceranae.
Results: Only N. ceranae was found in all samples, indicating that this species present in Iran apiaries.
Conclusion: This is the first report of N. ceranae in colonies of A. mellifera in Iran. It seems that intensive surveys are needed to determine the distribution and prevalence of N. ceranae in different regions of Iran.
|
topic |
Honeybee Nosema apis Nosema ceranae PCR |
url |
https://ijpa.tums.ac.ir/index.php/ijpa/article/view/193 |
work_keys_str_mv |
AT snabian firstdetectionofnosemaceranaeamicrosporidianprotozoaofeuropeanhoneybeesapismelliferainiran AT kahmadi firstdetectionofnosemaceranaeamicrosporidianprotozoaofeuropeanhoneybeesapismelliferainiran AT mhnazemshirazi firstdetectionofnosemaceranaeamicrosporidianprotozoaofeuropeanhoneybeesapismelliferainiran AT ageramisadeghian firstdetectionofnosemaceranaeamicrosporidianprotozoaofeuropeanhoneybeesapismelliferainiran |
_version_ |
1721563200291340288 |