Momordica charantia Extract Induces Apoptosis in Human Cancer Cells through Caspase- and Mitochondria-Dependent Pathways
Plants are an invaluable source of potential new anti-cancer drugs. Momordica charantia is one of these plants with both edible and medical value and reported to exhibit anticancer activity. To explore the potential effectiveness of Momordica charantia, methanol extract of Momordica charantia (MCME)...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2012-01-01
|
Series: | Evidence-Based Complementary and Alternative Medicine |
Online Access: | http://dx.doi.org/10.1155/2012/261971 |
id |
doaj-b7172bc94c6b4e369bf60f9c0bbaaed4 |
---|---|
record_format |
Article |
spelling |
doaj-b7172bc94c6b4e369bf60f9c0bbaaed42020-11-24T23:03:43ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882012-01-01201210.1155/2012/261971261971Momordica charantia Extract Induces Apoptosis in Human Cancer Cells through Caspase- and Mitochondria-Dependent PathwaysChia-Jung Li0Shih-Fang Tsang1Chun-Hao Tsai2Hsin-Yi Tsai3Jong-Ho Chyuan4Hsue-Yin Hsu5Institute of Medical Sciences, Tzu Chi University, Hualien 970, TaiwanDepartment of Anatomy, Tzu Chi University, Hualien 970, TaiwanDepartment of Life Sciences, Tzu Chi University, Hualien 970, TaiwanDepartment of Life Sciences, Tzu Chi University, Hualien 970, TaiwanSection of Crop Improvement, Hualien District Agricultural Research and Extension Station, Council of Agriculture, Executive Yuan, Hualien 973, TaiwanInstitute of Medical Sciences, Tzu Chi University, Hualien 970, TaiwanPlants are an invaluable source of potential new anti-cancer drugs. Momordica charantia is one of these plants with both edible and medical value and reported to exhibit anticancer activity. To explore the potential effectiveness of Momordica charantia, methanol extract of Momordica charantia (MCME) was used to evaluate the cytotoxic activity on four human cancer cell lines, Hone-1 nasopharyngeal carcinoma cells, AGS gastric adenocarcinoma cells, HCT-116 colorectal carcinoma cells, and CL1-0 lung adenocarcinoma cells, in this study. MCME showed cytotoxic activity towards all cancer cells tested, with the approximate IC50 ranging from 0.25 to 0.35 mg/mL at 24 h. MCME induced cell death was found to be time-dependent in these cells. Apoptosis was demonstrated by DAPI staining and DNA fragmentation analysis using agarose gel electrophoresis. MCME activated caspase-3 and enhanced the cleavage of downstream DFF45 and PARP, subsequently leading to DNA fragmentation and nuclear condensation. The apoptogenic protein, Bax, was increased, whereas Bcl-2 was decreased after treating for 24 h in all cancer cells, indicating the involvement of mitochondrial pathway in MCME-induced cell death. These findings indicate that MCME has cytotoxic effects on human cancer cells and exhibits promising anti-cancer activity by triggering apoptosis through the regulation of caspases and mitochondria.http://dx.doi.org/10.1155/2012/261971 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chia-Jung Li Shih-Fang Tsang Chun-Hao Tsai Hsin-Yi Tsai Jong-Ho Chyuan Hsue-Yin Hsu |
spellingShingle |
Chia-Jung Li Shih-Fang Tsang Chun-Hao Tsai Hsin-Yi Tsai Jong-Ho Chyuan Hsue-Yin Hsu Momordica charantia Extract Induces Apoptosis in Human Cancer Cells through Caspase- and Mitochondria-Dependent Pathways Evidence-Based Complementary and Alternative Medicine |
author_facet |
Chia-Jung Li Shih-Fang Tsang Chun-Hao Tsai Hsin-Yi Tsai Jong-Ho Chyuan Hsue-Yin Hsu |
author_sort |
Chia-Jung Li |
title |
Momordica charantia Extract Induces Apoptosis in Human Cancer Cells through Caspase- and Mitochondria-Dependent Pathways |
title_short |
Momordica charantia Extract Induces Apoptosis in Human Cancer Cells through Caspase- and Mitochondria-Dependent Pathways |
title_full |
Momordica charantia Extract Induces Apoptosis in Human Cancer Cells through Caspase- and Mitochondria-Dependent Pathways |
title_fullStr |
Momordica charantia Extract Induces Apoptosis in Human Cancer Cells through Caspase- and Mitochondria-Dependent Pathways |
title_full_unstemmed |
Momordica charantia Extract Induces Apoptosis in Human Cancer Cells through Caspase- and Mitochondria-Dependent Pathways |
title_sort |
momordica charantia extract induces apoptosis in human cancer cells through caspase- and mitochondria-dependent pathways |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2012-01-01 |
description |
Plants are an invaluable source of potential new anti-cancer drugs. Momordica charantia is one of these plants with both edible and medical value and reported to exhibit anticancer activity. To explore the potential effectiveness of Momordica charantia, methanol extract of Momordica charantia (MCME) was used to evaluate the cytotoxic activity on four human cancer cell lines, Hone-1 nasopharyngeal carcinoma cells, AGS gastric adenocarcinoma cells, HCT-116 colorectal carcinoma cells, and CL1-0 lung adenocarcinoma cells, in this study. MCME showed cytotoxic activity towards all cancer cells tested, with the approximate IC50 ranging from 0.25 to 0.35 mg/mL at 24 h. MCME induced cell death was found to be time-dependent in these cells. Apoptosis was demonstrated by DAPI staining and DNA fragmentation analysis using agarose gel electrophoresis. MCME activated caspase-3 and enhanced the cleavage of downstream DFF45 and PARP, subsequently leading to DNA fragmentation and nuclear condensation. The apoptogenic protein, Bax, was increased, whereas Bcl-2 was decreased after treating for 24 h in all cancer cells, indicating the involvement of mitochondrial pathway in MCME-induced cell death. These findings indicate that MCME has cytotoxic effects on human cancer cells and exhibits promising anti-cancer activity by triggering apoptosis through the regulation of caspases and mitochondria. |
url |
http://dx.doi.org/10.1155/2012/261971 |
work_keys_str_mv |
AT chiajungli momordicacharantiaextractinducesapoptosisinhumancancercellsthroughcaspaseandmitochondriadependentpathways AT shihfangtsang momordicacharantiaextractinducesapoptosisinhumancancercellsthroughcaspaseandmitochondriadependentpathways AT chunhaotsai momordicacharantiaextractinducesapoptosisinhumancancercellsthroughcaspaseandmitochondriadependentpathways AT hsinyitsai momordicacharantiaextractinducesapoptosisinhumancancercellsthroughcaspaseandmitochondriadependentpathways AT jonghochyuan momordicacharantiaextractinducesapoptosisinhumancancercellsthroughcaspaseandmitochondriadependentpathways AT hsueyinhsu momordicacharantiaextractinducesapoptosisinhumancancercellsthroughcaspaseandmitochondriadependentpathways |
_version_ |
1725632528441671680 |