Use of a new multiplex quantitative polymerase chain reaction based assay for simultaneous detection of Neisseria meningitidis, Escherichia coli K1, Streptococcus agalactiae, and Streptococcus pneumoniae
Background and Objectives: Neisseria meningitidis, Escherichia coli K1, Streptococcus agalactiae, and Streptococcus pneumoniae cause 90% of bacterial meningitis. Almost all infected people die or have irreversible neurological complications. Therefore, it is essential to have a diagnostic kit with...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Tehran University of Medical Sciences
2021-08-01
|
Series: | Iranian Journal of Microbiology |
Subjects: | |
Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/3059 |
id |
doaj-b71c2e0706644682bf635e59661fd26f |
---|---|
record_format |
Article |
spelling |
doaj-b71c2e0706644682bf635e59661fd26f2021-09-11T04:56:55ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472021-08-0113410.18502/ijm.v13i4.6970Use of a new multiplex quantitative polymerase chain reaction based assay for simultaneous detection of Neisseria meningitidis, Escherichia coli K1, Streptococcus agalactiae, and Streptococcus pneumoniaeNastaran Hemmati0Farhad Nikkhahi1Amir Javadi2Sahar Eskandarion3Seyed Mahmoud Amin Marashi4Student Research Committee, School of Medicine, Qazvin University of Medical Sciences, Qazvin, IranMedical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, IranDepartment of Community Medicine, Qazvin University of Medical Sciences, Qazvin, IranDepartment of Pediatric Medicine, Imam Hossein Hospital, Iran University of Medical Sciences, Tehran, IranMedical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran Background and Objectives: Neisseria meningitidis, Escherichia coli K1, Streptococcus agalactiae, and Streptococcus pneumoniae cause 90% of bacterial meningitis. Almost all infected people die or have irreversible neurological complications. Therefore, it is essential to have a diagnostic kit with the ability to quickly detect these fatal infections. Materials and Methods: The project involved 212 patients from whom cerebrospinal fluid samples were obtained. After total genome extraction and performing multiplex quantitative polymerase chain reaction (qPCR), the presence or absence of each infectious factor was determined by comparing with standard strains. Results: The specificity, sensitivity, positive predictive value, and negative predictive value calculated were 100%, 92.9%, 50%, and 100%, respectively. So, due to the high specificity and sensitivity of the designed primers, they can be used instead of bacterial culture that takes at least 24 to 48 hours. Conclusion: The remarkable benefit of this method is associated with the speed (up to 3 hours) at which the procedure could be completed. It is also worth noting that this method can reduce the personnel unintentional errors which may occur in the laboratory. On the other hand, as this method simultaneously identifies four common factors that cause bacterial meningitis, it could be used as an auxiliary method diagnostic technique in laboratories particularly in cases of emergency medicine. https://ijm.tums.ac.ir/index.php/ijm/article/view/3059Cerebrospinal fluid;Meningitis;Quantitative polymerase chain reaction;Simultaneous detection;Diagnosis testing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nastaran Hemmati Farhad Nikkhahi Amir Javadi Sahar Eskandarion Seyed Mahmoud Amin Marashi |
spellingShingle |
Nastaran Hemmati Farhad Nikkhahi Amir Javadi Sahar Eskandarion Seyed Mahmoud Amin Marashi Use of a new multiplex quantitative polymerase chain reaction based assay for simultaneous detection of Neisseria meningitidis, Escherichia coli K1, Streptococcus agalactiae, and Streptococcus pneumoniae Iranian Journal of Microbiology Cerebrospinal fluid; Meningitis; Quantitative polymerase chain reaction; Simultaneous detection; Diagnosis testing |
author_facet |
Nastaran Hemmati Farhad Nikkhahi Amir Javadi Sahar Eskandarion Seyed Mahmoud Amin Marashi |
author_sort |
Nastaran Hemmati |
title |
Use of a new multiplex quantitative polymerase chain reaction based assay for simultaneous detection of Neisseria meningitidis, Escherichia coli K1, Streptococcus agalactiae, and Streptococcus pneumoniae |
title_short |
Use of a new multiplex quantitative polymerase chain reaction based assay for simultaneous detection of Neisseria meningitidis, Escherichia coli K1, Streptococcus agalactiae, and Streptococcus pneumoniae |
title_full |
Use of a new multiplex quantitative polymerase chain reaction based assay for simultaneous detection of Neisseria meningitidis, Escherichia coli K1, Streptococcus agalactiae, and Streptococcus pneumoniae |
title_fullStr |
Use of a new multiplex quantitative polymerase chain reaction based assay for simultaneous detection of Neisseria meningitidis, Escherichia coli K1, Streptococcus agalactiae, and Streptococcus pneumoniae |
title_full_unstemmed |
Use of a new multiplex quantitative polymerase chain reaction based assay for simultaneous detection of Neisseria meningitidis, Escherichia coli K1, Streptococcus agalactiae, and Streptococcus pneumoniae |
title_sort |
use of a new multiplex quantitative polymerase chain reaction based assay for simultaneous detection of neisseria meningitidis, escherichia coli k1, streptococcus agalactiae, and streptococcus pneumoniae |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Microbiology |
issn |
2008-3289 2008-4447 |
publishDate |
2021-08-01 |
description |
Background and Objectives: Neisseria meningitidis, Escherichia coli K1, Streptococcus agalactiae, and Streptococcus pneumoniae cause 90% of bacterial meningitis. Almost all infected people die or have irreversible neurological complications. Therefore, it is essential to have a diagnostic kit with the ability to quickly detect these fatal infections.
Materials and Methods: The project involved 212 patients from whom cerebrospinal fluid samples were obtained. After total genome extraction and performing multiplex quantitative polymerase chain reaction (qPCR), the presence or absence of each infectious factor was determined by comparing with standard strains.
Results: The specificity, sensitivity, positive predictive value, and negative predictive value calculated were 100%, 92.9%, 50%, and 100%, respectively. So, due to the high specificity and sensitivity of the designed primers, they can be used instead of bacterial culture that takes at least 24 to 48 hours.
Conclusion: The remarkable benefit of this method is associated with the speed (up to 3 hours) at which the procedure could be completed. It is also worth noting that this method can reduce the personnel unintentional errors which may occur in the laboratory. On the other hand, as this method simultaneously identifies four common factors that cause bacterial meningitis, it could be used as an auxiliary method diagnostic technique in laboratories particularly in cases of emergency medicine.
|
topic |
Cerebrospinal fluid; Meningitis; Quantitative polymerase chain reaction; Simultaneous detection; Diagnosis testing |
url |
https://ijm.tums.ac.ir/index.php/ijm/article/view/3059 |
work_keys_str_mv |
AT nastaranhemmati useofanewmultiplexquantitativepolymerasechainreactionbasedassayforsimultaneousdetectionofneisseriameningitidisescherichiacolik1streptococcusagalactiaeandstreptococcuspneumoniae AT farhadnikkhahi useofanewmultiplexquantitativepolymerasechainreactionbasedassayforsimultaneousdetectionofneisseriameningitidisescherichiacolik1streptococcusagalactiaeandstreptococcuspneumoniae AT amirjavadi useofanewmultiplexquantitativepolymerasechainreactionbasedassayforsimultaneousdetectionofneisseriameningitidisescherichiacolik1streptococcusagalactiaeandstreptococcuspneumoniae AT sahareskandarion useofanewmultiplexquantitativepolymerasechainreactionbasedassayforsimultaneousdetectionofneisseriameningitidisescherichiacolik1streptococcusagalactiaeandstreptococcuspneumoniae AT seyedmahmoudaminmarashi useofanewmultiplexquantitativepolymerasechainreactionbasedassayforsimultaneousdetectionofneisseriameningitidisescherichiacolik1streptococcusagalactiaeandstreptococcuspneumoniae |
_version_ |
1717757096236154880 |