Lactobacillus reuteri in its biofilm state promotes neurodevelopment after experimental necrotizing enterocolitis in rats
Necrotizing enterocolitis (NEC) is a devastating disease affecting premature newborns with no known cure. Up to half of survivors subsequently exhibit cognitive impairment and neurodevelopmental defects. We created a novel probiotics delivery system in which the probiotic Lactobacillus reuteri (Lr)...
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Format: | Article |
Language: | English |
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Elsevier
2021-07-01
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Series: | Brain, Behavior, & Immunity - Health |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2666354621000594 |
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doaj-b71d192ed7914402930ad7d002ccbb88 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yijie Wang Robert M. Jaggers Pamela Mar Jeffrey D. Galley Terri Shaffer Adrian Rajab Shivani Deshpande Lauren Mashburn-Warren John R. Buzzo Steven D. Goodman Michael T. Bailey Gail E. Besner |
spellingShingle |
Yijie Wang Robert M. Jaggers Pamela Mar Jeffrey D. Galley Terri Shaffer Adrian Rajab Shivani Deshpande Lauren Mashburn-Warren John R. Buzzo Steven D. Goodman Michael T. Bailey Gail E. Besner Lactobacillus reuteri in its biofilm state promotes neurodevelopment after experimental necrotizing enterocolitis in rats Brain, Behavior, & Immunity - Health Necrotizing enterocolitis (NEC) Cognitive function Learning Memory Probiotics Lactobacillus reuteri |
author_facet |
Yijie Wang Robert M. Jaggers Pamela Mar Jeffrey D. Galley Terri Shaffer Adrian Rajab Shivani Deshpande Lauren Mashburn-Warren John R. Buzzo Steven D. Goodman Michael T. Bailey Gail E. Besner |
author_sort |
Yijie Wang |
title |
Lactobacillus reuteri in its biofilm state promotes neurodevelopment after experimental necrotizing enterocolitis in rats |
title_short |
Lactobacillus reuteri in its biofilm state promotes neurodevelopment after experimental necrotizing enterocolitis in rats |
title_full |
Lactobacillus reuteri in its biofilm state promotes neurodevelopment after experimental necrotizing enterocolitis in rats |
title_fullStr |
Lactobacillus reuteri in its biofilm state promotes neurodevelopment after experimental necrotizing enterocolitis in rats |
title_full_unstemmed |
Lactobacillus reuteri in its biofilm state promotes neurodevelopment after experimental necrotizing enterocolitis in rats |
title_sort |
lactobacillus reuteri in its biofilm state promotes neurodevelopment after experimental necrotizing enterocolitis in rats |
publisher |
Elsevier |
series |
Brain, Behavior, & Immunity - Health |
issn |
2666-3546 |
publishDate |
2021-07-01 |
description |
Necrotizing enterocolitis (NEC) is a devastating disease affecting premature newborns with no known cure. Up to half of survivors subsequently exhibit cognitive impairment and neurodevelopmental defects. We created a novel probiotics delivery system in which the probiotic Lactobacillus reuteri (Lr) was induced to form a biofilm [Lr (biofilm)] by incubation with dextranomer microspheres loaded with maltose (Lr-DM-maltose). We have previously demonstrated that a single dose of the probiotic Lr administered in its biofilm state significantly reduces the incidence of NEC and decreases inflammatory cytokine production in an animal model of the disease. The aim of our current study was to determine whether a single dose of the probiotic Lr administered in its biofilm state protects the brain after experimental NEC. We found that rat pups exposed to NEC reached developmental milestones significantly slower than breast fed pups, with mild improvement with Lr (biofilm) treatment. Exposure to NEC had a negative effect on cognitive behavior, which was prevented by Lr (biofilm) treatment. Lr administration also reduced anxiety-like behavior in NEC-exposed rats. The behavioral effects of NEC were associated with increased numbers of activated microglia, decreased myelin basic protein (MBP), and decreased neurotrophic gene expression, which were prevented by administration of Lr (biofilm). Our data indicate early enteral treatment with Lr in its biofilm state prevented the deleterious effects of NEC on developmental impairments. |
topic |
Necrotizing enterocolitis (NEC) Cognitive function Learning Memory Probiotics Lactobacillus reuteri |
url |
http://www.sciencedirect.com/science/article/pii/S2666354621000594 |
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doaj-b71d192ed7914402930ad7d002ccbb882021-06-10T04:58:17ZengElsevierBrain, Behavior, & Immunity - Health2666-35462021-07-0114100256Lactobacillus reuteri in its biofilm state promotes neurodevelopment after experimental necrotizing enterocolitis in ratsYijie Wang0Robert M. Jaggers1Pamela Mar2Jeffrey D. Galley3Terri Shaffer4Adrian Rajab5Shivani Deshpande6Lauren Mashburn-Warren7John R. Buzzo8Steven D. Goodman9Michael T. Bailey10Gail E. Besner11Center for Perinatal Research, The Research Institute at Nationwide Children’s Hospital, Department of Pediatric Surgery, Nationwide Children’s Hospital, 700 Children’s Drive, Columbus, OH, 43205, USACenter for Microbial Pathogenesis, Nationwide Children’s Hospital, 700 Children’s Drive, Columbus, OH, 43205, USACenter for Perinatal Research, The Research Institute at Nationwide Children’s Hospital, Department of Pediatric Surgery, Nationwide Children’s Hospital, 700 Children’s Drive, Columbus, OH, 43205, USACenter for Perinatal Research, The Research Institute at Nationwide Children’s Hospital, Department of Pediatric Surgery, Nationwide Children’s Hospital, 700 Children’s Drive, Columbus, OH, 43205, USAPreclinical Imaging and Behavior Core/Animal Resources Core, The Research Institute at Nationwide Children’s Hospital, 700 Children’s Drive, Columbus, OH, 43205, USACenter for Perinatal Research, The Research Institute at Nationwide Children’s Hospital, Department of Pediatric Surgery, Nationwide Children’s Hospital, 700 Children’s Drive, Columbus, OH, 43205, USACenter for Perinatal Research, The Research Institute at Nationwide Children’s Hospital, Department of Pediatric Surgery, Nationwide Children’s Hospital, 700 Children’s Drive, Columbus, OH, 43205, USACenter for Microbial Pathogenesis, Nationwide Children’s Hospital, 700 Children’s Drive, Columbus, OH, 43205, USACenter for Microbial Pathogenesis, Nationwide Children’s Hospital, 700 Children’s Drive, Columbus, OH, 43205, USACenter for Microbial Pathogenesis, Nationwide Children’s Hospital, 700 Children’s Drive, Columbus, OH, 43205, USACenter for Microbial Pathogenesis, Nationwide Children’s Hospital, 700 Children’s Drive, Columbus, OH, 43205, USACenter for Perinatal Research, The Research Institute at Nationwide Children’s Hospital, Department of Pediatric Surgery, Nationwide Children’s Hospital, 700 Children’s Drive, Columbus, OH, 43205, USA; Corresponding author. Department of Pediatric Surgery, FB6135, Nationwide Children’s Hospital, 700 Children’s Drive, Columbus, OH, 43205, USA.Necrotizing enterocolitis (NEC) is a devastating disease affecting premature newborns with no known cure. Up to half of survivors subsequently exhibit cognitive impairment and neurodevelopmental defects. We created a novel probiotics delivery system in which the probiotic Lactobacillus reuteri (Lr) was induced to form a biofilm [Lr (biofilm)] by incubation with dextranomer microspheres loaded with maltose (Lr-DM-maltose). We have previously demonstrated that a single dose of the probiotic Lr administered in its biofilm state significantly reduces the incidence of NEC and decreases inflammatory cytokine production in an animal model of the disease. The aim of our current study was to determine whether a single dose of the probiotic Lr administered in its biofilm state protects the brain after experimental NEC. We found that rat pups exposed to NEC reached developmental milestones significantly slower than breast fed pups, with mild improvement with Lr (biofilm) treatment. Exposure to NEC had a negative effect on cognitive behavior, which was prevented by Lr (biofilm) treatment. Lr administration also reduced anxiety-like behavior in NEC-exposed rats. The behavioral effects of NEC were associated with increased numbers of activated microglia, decreased myelin basic protein (MBP), and decreased neurotrophic gene expression, which were prevented by administration of Lr (biofilm). Our data indicate early enteral treatment with Lr in its biofilm state prevented the deleterious effects of NEC on developmental impairments.http://www.sciencedirect.com/science/article/pii/S2666354621000594Necrotizing enterocolitis (NEC)Cognitive functionLearningMemoryProbioticsLactobacillus reuteri |