RHON1 Co-transcriptionally Resolves R-Loops for Arabidopsis Chloroplast Genome Maintenance
Summary: Preventing transcription-replication head-on conflict (HO-TRC)-triggered R-loop formation is essential for maintaining genome integrity in bacteria, plants, and mammals. The R-loop eraser RNase H can efficiently relax HO-TRCs. However, it is not clear how organisms resist HO-TRC-triggered R...
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doaj-b738da2d69e1400084ec2458ef5699a72020-11-25T02:44:55ZengElsevierCell Reports2211-12472020-01-01301243256.e5RHON1 Co-transcriptionally Resolves R-Loops for Arabidopsis Chloroplast Genome MaintenanceZhuo Yang0Mengmeng Li1Qianwen Sun2Tsinghua-Peking Joint Center for Life Sciences and Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, ChinaTsinghua-Peking Joint Center for Life Sciences and Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, ChinaTsinghua-Peking Joint Center for Life Sciences and Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China; Corresponding authorSummary: Preventing transcription-replication head-on conflict (HO-TRC)-triggered R-loop formation is essential for maintaining genome integrity in bacteria, plants, and mammals. The R-loop eraser RNase H can efficiently relax HO-TRCs. However, it is not clear how organisms resist HO-TRC-triggered R-loops when RNase H proteins are deficient. By screening factors that may relieve R-loop accumulation in the Arabidopsis atrnh1c mutant, we find that overexpression of the R-loop helicase RHON1 can rescue the defects of aberrantly accumulated HO-TRC-triggered R-loops co-transcriptionally. In addition, we find that RHON1 interacts with and orchestrates the transcriptional activity of plastid-encoded RNA polymerases to release the conflicts between transcription and replication. Our study illustrates that organisms employ multiple mechanisms to escape HO-TRC-triggered R-loop accumulation and thus maintain genome integrity. : Preventing transcription-replication head-on conflict (HO-TRC)-triggered R-loops is essential for genome maintenance in bacteria, plants, and mammals. Yang et al. unveil a bacterial Rho-like DNA:RNA helicase, RHON1, that functions in parallel with RNase H1 to restrict HO-TRC-promoted R-loops and stabilize the genome in Arabidopsis chloroplasts, the endosymbiotic organelle that is essential for photosynthesis. Keywords: R-loops, transcription-replication conflicts, head-on, RHON1, organelle-encoded RNA polymerase, RNase H1http://www.sciencedirect.com/science/article/pii/S2211124719316468 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhuo Yang Mengmeng Li Qianwen Sun |
spellingShingle |
Zhuo Yang Mengmeng Li Qianwen Sun RHON1 Co-transcriptionally Resolves R-Loops for Arabidopsis Chloroplast Genome Maintenance Cell Reports |
author_facet |
Zhuo Yang Mengmeng Li Qianwen Sun |
author_sort |
Zhuo Yang |
title |
RHON1 Co-transcriptionally Resolves R-Loops for Arabidopsis Chloroplast Genome Maintenance |
title_short |
RHON1 Co-transcriptionally Resolves R-Loops for Arabidopsis Chloroplast Genome Maintenance |
title_full |
RHON1 Co-transcriptionally Resolves R-Loops for Arabidopsis Chloroplast Genome Maintenance |
title_fullStr |
RHON1 Co-transcriptionally Resolves R-Loops for Arabidopsis Chloroplast Genome Maintenance |
title_full_unstemmed |
RHON1 Co-transcriptionally Resolves R-Loops for Arabidopsis Chloroplast Genome Maintenance |
title_sort |
rhon1 co-transcriptionally resolves r-loops for arabidopsis chloroplast genome maintenance |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2020-01-01 |
description |
Summary: Preventing transcription-replication head-on conflict (HO-TRC)-triggered R-loop formation is essential for maintaining genome integrity in bacteria, plants, and mammals. The R-loop eraser RNase H can efficiently relax HO-TRCs. However, it is not clear how organisms resist HO-TRC-triggered R-loops when RNase H proteins are deficient. By screening factors that may relieve R-loop accumulation in the Arabidopsis atrnh1c mutant, we find that overexpression of the R-loop helicase RHON1 can rescue the defects of aberrantly accumulated HO-TRC-triggered R-loops co-transcriptionally. In addition, we find that RHON1 interacts with and orchestrates the transcriptional activity of plastid-encoded RNA polymerases to release the conflicts between transcription and replication. Our study illustrates that organisms employ multiple mechanisms to escape HO-TRC-triggered R-loop accumulation and thus maintain genome integrity. : Preventing transcription-replication head-on conflict (HO-TRC)-triggered R-loops is essential for genome maintenance in bacteria, plants, and mammals. Yang et al. unveil a bacterial Rho-like DNA:RNA helicase, RHON1, that functions in parallel with RNase H1 to restrict HO-TRC-promoted R-loops and stabilize the genome in Arabidopsis chloroplasts, the endosymbiotic organelle that is essential for photosynthesis. Keywords: R-loops, transcription-replication conflicts, head-on, RHON1, organelle-encoded RNA polymerase, RNase H1 |
url |
http://www.sciencedirect.com/science/article/pii/S2211124719316468 |
work_keys_str_mv |
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