Association among serum and salivary A. actinomycetemcomitans specific immunoglobulin antibodies and periodontitis
Abstract Background The aim of this study was to assess the association between serum and salivary Immunoglobulin (Ig) Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) specific antibodies in healthy controls (HC) and periodontitis (PT) patients. Furthermore, the objectives were to de...
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doaj-b74e2ca22dad461c90529667a488c5542020-11-25T03:51:57ZengBMCBMC Oral Health1472-68312020-10-0120111310.1186/s12903-020-01258-5Association among serum and salivary A. actinomycetemcomitans specific immunoglobulin antibodies and periodontitisGaetano Isola0Alessandro Polizzi1Romeo Patini2Sebastiano Ferlito3Angela Alibrandi4Giuseppe Palazzo5Department of General Surgery and Surgical-Medical Specialties, School of Dentistry, University of CataniaDepartment of General Surgery and Surgical-Medical Specialties, School of Dentistry, University of CataniaFondazione Policlinico Universitario A. Gemelli IRCCS, Institute of Dentistry and Maxillofacial Surgery, Università Cattolica del Sacro CuoreDepartment of General Surgery and Surgical-Medical Specialties, School of Dentistry, University of CataniaDepartment of Economical, Business and Environmental Sciences and Quantitative Methods, University of MessinaDepartment of General Surgery and Surgical-Medical Specialties, School of Dentistry, University of CataniaAbstract Background The aim of this study was to assess the association between serum and salivary Immunoglobulin (Ig) Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) specific antibodies in healthy controls (HC) and periodontitis (PT) patients. Furthermore, the objectives were to determine whether PT influenced serum A. actinomycetemcomitans specific antibodies and whether serum or salivary antibodies against A. actinomycetemcomitans IgG were mediated by serum high-sensitivity c-reactive protein (hs-CRP). Methods Fifty-three patients with periodontitis and 48 HC were enrolled in the present study. Patients were regularly examined and characterized by clinical, salivary and blood samples analyses. A. actinomycetemcomitans IgA and IgG antibodies and hs-CRP were evaluated using a commercially available kit. The Spearman Correlation Test and Jonckheere-Terpstra Test were applied in order to assess the interdependence between serum A. actinomycetemcomitans IgG antibodies and clinical periodontal parameters. To evaluate the dependence of the serum and salivary A. actinomycetemcomitans IgG levels from possible confounders, univariate and multivariable linear regression analyses were performed. Results Compared to HC, patients with PT had significantly higher IgA [serum: PT, 1.89 (1.2–2.2) EU vs HC, 1.37 (0.9–1.8) EU (p = 0.022); saliva: PT, 1.67 (1.4–2.1) EU vs HC, 1.42 (0.9–1.6) EU (p = 0.019)] and A. actinomycetemcomitans IgG levels [serum: PT, 2.96 (2.1–3.7) EU vs HC, 2.18 (1.8–2.1) EU (p < 0.001); saliva, PT, 2.19 (1.8–2.5) EU vs HC, 1.84 (1.4–2) EU (p = 0.028)]. In PT patients, serum A. actinomycetemcomitans IgG were associated with a proportional extent of PT and tooth loss (P-trend value< 0.001). The univariate regression analysis demonstrated that PT (p = 0.013) and high hs-CRP (p < 0.001) had a significant negative effect on serum and salivary A. actinomycetemcomitans IgG levels. The multivariate regression analysis showed that PT (p = 0.033), hs-CRP (p = 0.014) and BMI (p = 0.017) were significant negative predictors of serum A. actinomycetemcomitans IgG while hs-CRP (p < 0.001) and BMI (P = 0.025) were significant negative predictors of salivary A. actinomycetemcomitans IgG. Conclusions PT patients presented a significantly higher serum and salivary A. actinomycetemcomitans IgA and IgG compared to HC. There was a significant increase in serum A. actinomycetemcomitans IgG when patients presented a progressive extent of PT. Moreover, PT and hs-CRP were significant negative predictors of increased salivary and serum A. actinomycetemcomitans IgG levels. Trial registration The study was retrospectively registered at clinicaltrials.gov ( NCT04417322 ).http://link.springer.com/article/10.1186/s12903-020-01258-5 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gaetano Isola Alessandro Polizzi Romeo Patini Sebastiano Ferlito Angela Alibrandi Giuseppe Palazzo |
spellingShingle |
Gaetano Isola Alessandro Polizzi Romeo Patini Sebastiano Ferlito Angela Alibrandi Giuseppe Palazzo Association among serum and salivary A. actinomycetemcomitans specific immunoglobulin antibodies and periodontitis BMC Oral Health |
author_facet |
Gaetano Isola Alessandro Polizzi Romeo Patini Sebastiano Ferlito Angela Alibrandi Giuseppe Palazzo |
author_sort |
Gaetano Isola |
title |
Association among serum and salivary A. actinomycetemcomitans specific immunoglobulin antibodies and periodontitis |
title_short |
Association among serum and salivary A. actinomycetemcomitans specific immunoglobulin antibodies and periodontitis |
title_full |
Association among serum and salivary A. actinomycetemcomitans specific immunoglobulin antibodies and periodontitis |
title_fullStr |
Association among serum and salivary A. actinomycetemcomitans specific immunoglobulin antibodies and periodontitis |
title_full_unstemmed |
Association among serum and salivary A. actinomycetemcomitans specific immunoglobulin antibodies and periodontitis |
title_sort |
association among serum and salivary a. actinomycetemcomitans specific immunoglobulin antibodies and periodontitis |
publisher |
BMC |
series |
BMC Oral Health |
issn |
1472-6831 |
publishDate |
2020-10-01 |
description |
Abstract Background The aim of this study was to assess the association between serum and salivary Immunoglobulin (Ig) Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) specific antibodies in healthy controls (HC) and periodontitis (PT) patients. Furthermore, the objectives were to determine whether PT influenced serum A. actinomycetemcomitans specific antibodies and whether serum or salivary antibodies against A. actinomycetemcomitans IgG were mediated by serum high-sensitivity c-reactive protein (hs-CRP). Methods Fifty-three patients with periodontitis and 48 HC were enrolled in the present study. Patients were regularly examined and characterized by clinical, salivary and blood samples analyses. A. actinomycetemcomitans IgA and IgG antibodies and hs-CRP were evaluated using a commercially available kit. The Spearman Correlation Test and Jonckheere-Terpstra Test were applied in order to assess the interdependence between serum A. actinomycetemcomitans IgG antibodies and clinical periodontal parameters. To evaluate the dependence of the serum and salivary A. actinomycetemcomitans IgG levels from possible confounders, univariate and multivariable linear regression analyses were performed. Results Compared to HC, patients with PT had significantly higher IgA [serum: PT, 1.89 (1.2–2.2) EU vs HC, 1.37 (0.9–1.8) EU (p = 0.022); saliva: PT, 1.67 (1.4–2.1) EU vs HC, 1.42 (0.9–1.6) EU (p = 0.019)] and A. actinomycetemcomitans IgG levels [serum: PT, 2.96 (2.1–3.7) EU vs HC, 2.18 (1.8–2.1) EU (p < 0.001); saliva, PT, 2.19 (1.8–2.5) EU vs HC, 1.84 (1.4–2) EU (p = 0.028)]. In PT patients, serum A. actinomycetemcomitans IgG were associated with a proportional extent of PT and tooth loss (P-trend value< 0.001). The univariate regression analysis demonstrated that PT (p = 0.013) and high hs-CRP (p < 0.001) had a significant negative effect on serum and salivary A. actinomycetemcomitans IgG levels. The multivariate regression analysis showed that PT (p = 0.033), hs-CRP (p = 0.014) and BMI (p = 0.017) were significant negative predictors of serum A. actinomycetemcomitans IgG while hs-CRP (p < 0.001) and BMI (P = 0.025) were significant negative predictors of salivary A. actinomycetemcomitans IgG. Conclusions PT patients presented a significantly higher serum and salivary A. actinomycetemcomitans IgA and IgG compared to HC. There was a significant increase in serum A. actinomycetemcomitans IgG when patients presented a progressive extent of PT. Moreover, PT and hs-CRP were significant negative predictors of increased salivary and serum A. actinomycetemcomitans IgG levels. Trial registration The study was retrospectively registered at clinicaltrials.gov ( NCT04417322 ). |
url |
http://link.springer.com/article/10.1186/s12903-020-01258-5 |
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