Asymmetric Distribution of GFAP in Glioma Multipotent Cells.
Asymmetric division (AD) is a fundamental mechanism whereby unequal inheritance of various cellular compounds during mitosis generates unequal fate in the two daughter cells. Unequal repartitions of transcription factors, receptors as well as mRNA have been abundantly described in AD. In contrast, t...
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doaj-b753b8d699d642c7bff2297a8404c5572020-11-25T02:06:34ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01113e015127410.1371/journal.pone.0151274Asymmetric Distribution of GFAP in Glioma Multipotent Cells.Pierre-Olivier GuichetSophie GuelfiChantal RipollMarisa TeigellJean-Charles SabourinLuc BauchetValérie RigauBernard RothhutJean-Philippe HugnotAsymmetric division (AD) is a fundamental mechanism whereby unequal inheritance of various cellular compounds during mitosis generates unequal fate in the two daughter cells. Unequal repartitions of transcription factors, receptors as well as mRNA have been abundantly described in AD. In contrast, the involvement of intermediate filaments in this process is still largely unknown. AD occurs in stem cells during development but was also recently observed in cancer stem cells. Here, we demonstrate the asymmetric distribution of the main astrocytic intermediate filament, namely the glial fibrillary acid protein (GFAP), in mitotic glioma multipotent cells isolated from glioblastoma (GBM), the most frequent type of brain tumor. Unequal mitotic repartition of GFAP was also observed in mice non-tumoral neural stem cells indicating that this process occurs across species and is not restricted to cancerous cells. Immunofluorescence and videomicroscopy were used to capture these rare and transient events. Considering the role of intermediate filaments in cytoplasm organization and cell signaling, we propose that asymmetric distribution of GFAP could possibly participate in the regulation of normal and cancerous neural stem cell fate.http://europepmc.org/articles/PMC4783030?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pierre-Olivier Guichet Sophie Guelfi Chantal Ripoll Marisa Teigell Jean-Charles Sabourin Luc Bauchet Valérie Rigau Bernard Rothhut Jean-Philippe Hugnot |
spellingShingle |
Pierre-Olivier Guichet Sophie Guelfi Chantal Ripoll Marisa Teigell Jean-Charles Sabourin Luc Bauchet Valérie Rigau Bernard Rothhut Jean-Philippe Hugnot Asymmetric Distribution of GFAP in Glioma Multipotent Cells. PLoS ONE |
author_facet |
Pierre-Olivier Guichet Sophie Guelfi Chantal Ripoll Marisa Teigell Jean-Charles Sabourin Luc Bauchet Valérie Rigau Bernard Rothhut Jean-Philippe Hugnot |
author_sort |
Pierre-Olivier Guichet |
title |
Asymmetric Distribution of GFAP in Glioma Multipotent Cells. |
title_short |
Asymmetric Distribution of GFAP in Glioma Multipotent Cells. |
title_full |
Asymmetric Distribution of GFAP in Glioma Multipotent Cells. |
title_fullStr |
Asymmetric Distribution of GFAP in Glioma Multipotent Cells. |
title_full_unstemmed |
Asymmetric Distribution of GFAP in Glioma Multipotent Cells. |
title_sort |
asymmetric distribution of gfap in glioma multipotent cells. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2016-01-01 |
description |
Asymmetric division (AD) is a fundamental mechanism whereby unequal inheritance of various cellular compounds during mitosis generates unequal fate in the two daughter cells. Unequal repartitions of transcription factors, receptors as well as mRNA have been abundantly described in AD. In contrast, the involvement of intermediate filaments in this process is still largely unknown. AD occurs in stem cells during development but was also recently observed in cancer stem cells. Here, we demonstrate the asymmetric distribution of the main astrocytic intermediate filament, namely the glial fibrillary acid protein (GFAP), in mitotic glioma multipotent cells isolated from glioblastoma (GBM), the most frequent type of brain tumor. Unequal mitotic repartition of GFAP was also observed in mice non-tumoral neural stem cells indicating that this process occurs across species and is not restricted to cancerous cells. Immunofluorescence and videomicroscopy were used to capture these rare and transient events. Considering the role of intermediate filaments in cytoplasm organization and cell signaling, we propose that asymmetric distribution of GFAP could possibly participate in the regulation of normal and cancerous neural stem cell fate. |
url |
http://europepmc.org/articles/PMC4783030?pdf=render |
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