Silicate-substituted strontium apatite nano coating improves osteogenesis around artificial ligament
Abstract Background Treatment of anterior cruciate ligament injuries commonly involves the use of polyethylene terephthalate (PET) artificial ligaments for reconstruction. However, the currently available methods require long fixation periods, thereby necessitating the development of alternative met...
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doaj-b75892263b2141a2a4e86c6e15f031632020-11-25T03:48:49ZengBMCBMC Musculoskeletal Disorders1471-24742019-08-012011910.1186/s12891-019-2777-8Silicate-substituted strontium apatite nano coating improves osteogenesis around artificial ligamentTakuya Egawa0Yusuke Inagaki1Manabu Akahane2Akira Furukawa3Kazuya Inoue4Munehiro Ogawa5Yasuhito Tanaka6Department of Orthopedic Surgery, Nara Medical UniversityDepartment of Artificial Joint and Regenerative Medicine for Bone and Cartilage, Nara Medical UniversityDepartment of Public Health, Health Management and Policy, Nara Medical UniversityDepartment of Orthopedic Surgery, Nara Medical UniversityDepartment of Orthopedic Surgery, Nara Medical UniversityDepartment of Orthopedic Surgery, Nara Medical UniversityDepartment of Orthopedic Surgery, Nara Medical UniversityAbstract Background Treatment of anterior cruciate ligament injuries commonly involves the use of polyethylene terephthalate (PET) artificial ligaments for reconstruction. However, the currently available methods require long fixation periods, thereby necessitating the development of alternative methods to accelerate the healing process between tendons and bones. Thus, we developed and evaluated a novel technique that utilizes silicate-substituted strontium (SrSiP). Methods PET films, nano-coated with SrSiP, were prepared. Bone marrow mesenchymal cells (BMSCs) from femurs of male rats were cultured and seeded at a density of 1.0 × 104/cm2 onto the SrSiP-coated and non-coated PET film, and subsequently placed in an osteogenic medium. The osteocalcin concentration secreted into the medium was compared in each case. Next, PET artificial ligament, nano-coated with SrSiP, were prepared. BMSCs were seeded at a density of 4.5 × 105/cm2 onto the SrSiP-coated, and non-coated artificial ligament, and then placed in osteogenic medium. The osteocalcin and calcium concentrations in the culture medium were measured on the 8th, 10th, 12th, and 14th day of culture. Furthermore, mRNA expression of osteocalcin, alkaline phosphatase (ALP), bone morphogenetic protein-2 (BMP2), and runt-related transcription factor 2 (Runx2) was evaluated by qPCR. We transplanted the SrSiP-coated and non-coated artificial ligament to the tibiae of mature New Zealand white rabbits. Two months later, we sacrificed them and histologically evaluated them. Results The secretory osteocalcin concentration in the medium on the film was significantly higher for the SrSiP group than for the non-coated group. Secretory osteocalcin concentration in the medium on the artificial ligament was also significantly higher in the SrSiP group than in the non-coated group on the 14th day. Calcium concentration on the artificial ligament was significantly lower in the SrSiP group than in the non-coated group on the 8th, 10th, 12th, and 14th day. In qPCR as well, OC, ALP, BMP2, and Runx2 mRNA expression were significantly higher in the SrSiP group than in the non-coated group. Newly formed bone was histologically found around the artificial ligament in the SrSiP group. Conclusions Our findings demonstrate that artificial ligaments using SrSiP display high osteogenic potential and thus may be efficiently used in future clinical applications.http://link.springer.com/article/10.1186/s12891-019-2777-8Silicate-substituted strontiumAnterior cruciate ligament injuriesNano coatingOsteogenesisPET artificial ligament |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Takuya Egawa Yusuke Inagaki Manabu Akahane Akira Furukawa Kazuya Inoue Munehiro Ogawa Yasuhito Tanaka |
spellingShingle |
Takuya Egawa Yusuke Inagaki Manabu Akahane Akira Furukawa Kazuya Inoue Munehiro Ogawa Yasuhito Tanaka Silicate-substituted strontium apatite nano coating improves osteogenesis around artificial ligament BMC Musculoskeletal Disorders Silicate-substituted strontium Anterior cruciate ligament injuries Nano coating Osteogenesis PET artificial ligament |
author_facet |
Takuya Egawa Yusuke Inagaki Manabu Akahane Akira Furukawa Kazuya Inoue Munehiro Ogawa Yasuhito Tanaka |
author_sort |
Takuya Egawa |
title |
Silicate-substituted strontium apatite nano coating improves osteogenesis around artificial ligament |
title_short |
Silicate-substituted strontium apatite nano coating improves osteogenesis around artificial ligament |
title_full |
Silicate-substituted strontium apatite nano coating improves osteogenesis around artificial ligament |
title_fullStr |
Silicate-substituted strontium apatite nano coating improves osteogenesis around artificial ligament |
title_full_unstemmed |
Silicate-substituted strontium apatite nano coating improves osteogenesis around artificial ligament |
title_sort |
silicate-substituted strontium apatite nano coating improves osteogenesis around artificial ligament |
publisher |
BMC |
series |
BMC Musculoskeletal Disorders |
issn |
1471-2474 |
publishDate |
2019-08-01 |
description |
Abstract Background Treatment of anterior cruciate ligament injuries commonly involves the use of polyethylene terephthalate (PET) artificial ligaments for reconstruction. However, the currently available methods require long fixation periods, thereby necessitating the development of alternative methods to accelerate the healing process between tendons and bones. Thus, we developed and evaluated a novel technique that utilizes silicate-substituted strontium (SrSiP). Methods PET films, nano-coated with SrSiP, were prepared. Bone marrow mesenchymal cells (BMSCs) from femurs of male rats were cultured and seeded at a density of 1.0 × 104/cm2 onto the SrSiP-coated and non-coated PET film, and subsequently placed in an osteogenic medium. The osteocalcin concentration secreted into the medium was compared in each case. Next, PET artificial ligament, nano-coated with SrSiP, were prepared. BMSCs were seeded at a density of 4.5 × 105/cm2 onto the SrSiP-coated, and non-coated artificial ligament, and then placed in osteogenic medium. The osteocalcin and calcium concentrations in the culture medium were measured on the 8th, 10th, 12th, and 14th day of culture. Furthermore, mRNA expression of osteocalcin, alkaline phosphatase (ALP), bone morphogenetic protein-2 (BMP2), and runt-related transcription factor 2 (Runx2) was evaluated by qPCR. We transplanted the SrSiP-coated and non-coated artificial ligament to the tibiae of mature New Zealand white rabbits. Two months later, we sacrificed them and histologically evaluated them. Results The secretory osteocalcin concentration in the medium on the film was significantly higher for the SrSiP group than for the non-coated group. Secretory osteocalcin concentration in the medium on the artificial ligament was also significantly higher in the SrSiP group than in the non-coated group on the 14th day. Calcium concentration on the artificial ligament was significantly lower in the SrSiP group than in the non-coated group on the 8th, 10th, 12th, and 14th day. In qPCR as well, OC, ALP, BMP2, and Runx2 mRNA expression were significantly higher in the SrSiP group than in the non-coated group. Newly formed bone was histologically found around the artificial ligament in the SrSiP group. Conclusions Our findings demonstrate that artificial ligaments using SrSiP display high osteogenic potential and thus may be efficiently used in future clinical applications. |
topic |
Silicate-substituted strontium Anterior cruciate ligament injuries Nano coating Osteogenesis PET artificial ligament |
url |
http://link.springer.com/article/10.1186/s12891-019-2777-8 |
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