An in vivo study of Hypericum perforatum in a niosomal topical drug delivery system
The active compounds present in Hypericum perforatum L. (Hypericaceae) include hyperforin, hypericins and flavonoids, which are assumed to be responsible for the activity of the extract in the treatment of wounds and scars. The present study aimed to incorporate H. perforatum extract standardized to...
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doaj-b75903f7f6e9440e924b1e00b24eac422020-11-25T02:38:17ZengTaylor & Francis GroupDrug Delivery1071-75441521-04642018-01-0125141742510.1080/10717544.2018.14319771431977An in vivo study of Hypericum perforatum in a niosomal topical drug delivery systemMohamed Ali0Amira Abdel Motaal1Mohammed A. Ahmed2Abdulrhman Alsayari3Omaima N. El-Gazayerly4Atos PharmaCairo UniversityCairo UniversityKing Khaled UniversityCairo UniversityThe active compounds present in Hypericum perforatum L. (Hypericaceae) include hyperforin, hypericins and flavonoids, which are assumed to be responsible for the activity of the extract in the treatment of wounds and scars. The present study aimed to incorporate H. perforatum extract standardized to a known content of phloroglucinols, naphthodianthrones and polyphenolic compounds into an effective transdermal drug delivery system capable of entrapping both lipophilic and hydrophilic constituents in the form of niosomal gels for wound treatment. An 80% ethanol extract (HE) was prepared on a pilot scale using DIG-MAZ. An HPLC-DAD holistic profile was established for HE and was standardized to contain 3.4 ± 4 rutin, 1.1 ± 3 chlorogenic acid, 0.5 ± 2 quercitrin, 2.8 ± 2 hyperforin, and 0.51 ± 3% w/w total hypericins. Niosomes were prepared using the modified reverse phase evaporation technique (REV). The wound healing effect of the gel was tested on 16 adult mongrel dogs. A significant decrease in the inflammatory cell count (18.4 ± 5.3) was recorded in the niosomal gel 1.5% NaCMC-treated group at the 7th day post wounding. It induced a marked regression in the inflammatory phase and enhanced the early beginning of the proliferative phase of wound healing. After 21 days, it showed complete re-epithelization, formation of new matrix fibers and significant reduction in the wound size, compared to the control and the Panthenol® 2% cream treated groups. This is the first study of H. perforatum in a niosomal topical drug delivery system.http://dx.doi.org/10.1080/10717544.2018.1431977hypericum perforatumniosomesin vivostandardizationwound |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mohamed Ali Amira Abdel Motaal Mohammed A. Ahmed Abdulrhman Alsayari Omaima N. El-Gazayerly |
spellingShingle |
Mohamed Ali Amira Abdel Motaal Mohammed A. Ahmed Abdulrhman Alsayari Omaima N. El-Gazayerly An in vivo study of Hypericum perforatum in a niosomal topical drug delivery system Drug Delivery hypericum perforatum niosomes in vivo standardization wound |
author_facet |
Mohamed Ali Amira Abdel Motaal Mohammed A. Ahmed Abdulrhman Alsayari Omaima N. El-Gazayerly |
author_sort |
Mohamed Ali |
title |
An in vivo study of Hypericum perforatum in a niosomal topical drug delivery system |
title_short |
An in vivo study of Hypericum perforatum in a niosomal topical drug delivery system |
title_full |
An in vivo study of Hypericum perforatum in a niosomal topical drug delivery system |
title_fullStr |
An in vivo study of Hypericum perforatum in a niosomal topical drug delivery system |
title_full_unstemmed |
An in vivo study of Hypericum perforatum in a niosomal topical drug delivery system |
title_sort |
in vivo study of hypericum perforatum in a niosomal topical drug delivery system |
publisher |
Taylor & Francis Group |
series |
Drug Delivery |
issn |
1071-7544 1521-0464 |
publishDate |
2018-01-01 |
description |
The active compounds present in Hypericum perforatum L. (Hypericaceae) include hyperforin, hypericins and flavonoids, which are assumed to be responsible for the activity of the extract in the treatment of wounds and scars. The present study aimed to incorporate H. perforatum extract standardized to a known content of phloroglucinols, naphthodianthrones and polyphenolic compounds into an effective transdermal drug delivery system capable of entrapping both lipophilic and hydrophilic constituents in the form of niosomal gels for wound treatment. An 80% ethanol extract (HE) was prepared on a pilot scale using DIG-MAZ. An HPLC-DAD holistic profile was established for HE and was standardized to contain 3.4 ± 4 rutin, 1.1 ± 3 chlorogenic acid, 0.5 ± 2 quercitrin, 2.8 ± 2 hyperforin, and 0.51 ± 3% w/w total hypericins. Niosomes were prepared using the modified reverse phase evaporation technique (REV). The wound healing effect of the gel was tested on 16 adult mongrel dogs. A significant decrease in the inflammatory cell count (18.4 ± 5.3) was recorded in the niosomal gel 1.5% NaCMC-treated group at the 7th day post wounding. It induced a marked regression in the inflammatory phase and enhanced the early beginning of the proliferative phase of wound healing. After 21 days, it showed complete re-epithelization, formation of new matrix fibers and significant reduction in the wound size, compared to the control and the Panthenol® 2% cream treated groups. This is the first study of H. perforatum in a niosomal topical drug delivery system. |
topic |
hypericum perforatum niosomes in vivo standardization wound |
url |
http://dx.doi.org/10.1080/10717544.2018.1431977 |
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