A Vortex-Assisted Dispersive Liquid-Liquid Microextraction Followed by UPLC-MS/MS for Simultaneous Determination of Pesticides and Aflatoxins in Herbal Tea
A method for detecting the organophosphorus pesticides residue and aflatoxins in China herbal tea has been developed by UPLC-MS/MS coupled with vortex-assisted dispersive liquid-liquid microextraction (DLLME). The extraction conditions for vortex-assisted DLLME extraction were optimized using single...
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doaj-b75a8e8e2d3346be8b8602fb80fb8ced2020-11-25T02:16:32ZengMDPI AGMolecules1420-30492019-03-01246102910.3390/molecules24061029molecules24061029A Vortex-Assisted Dispersive Liquid-Liquid Microextraction Followed by UPLC-MS/MS for Simultaneous Determination of Pesticides and Aflatoxins in Herbal TeaRui Zhang0Zhen-Chao Tan1Ke-Cheng Huang2Yan Wen3Xiang-Ying Li4Jun-Long Zhao5Cheng-Lan Liu6Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Agriculture & Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, South China Agricultural University, Wushan Road 483, Guangzhou 510642, ChinaKey Laboratory of Natural Pesticide and Chemical Biology, Ministry of Agriculture & Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, South China Agricultural University, Wushan Road 483, Guangzhou 510642, ChinaShenzhen Noposion Agrochemical Co. Ltd., Shenzhen 510640, Guangdong, ChinaKey Laboratory of Natural Pesticide and Chemical Biology, Ministry of Agriculture & Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, South China Agricultural University, Wushan Road 483, Guangzhou 510642, ChinaKey Laboratory of Natural Pesticide and Chemical Biology, Ministry of Agriculture & Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, South China Agricultural University, Wushan Road 483, Guangzhou 510642, ChinaKey Laboratory of Natural Pesticide and Chemical Biology, Ministry of Agriculture & Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, South China Agricultural University, Wushan Road 483, Guangzhou 510642, ChinaKey Laboratory of Natural Pesticide and Chemical Biology, Ministry of Agriculture & Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, South China Agricultural University, Wushan Road 483, Guangzhou 510642, ChinaA method for detecting the organophosphorus pesticides residue and aflatoxins in China herbal tea has been developed by UPLC-MS/MS coupled with vortex-assisted dispersive liquid-liquid microextraction (DLLME). The extraction conditions for vortex-assisted DLLME extraction were optimized using single-factor experiments and response surface design. The optimum conditions for the experiment were the pH 5.1, 347 µL of chloroform (extraction solvent) and 1614 µL of acetonitrile (dispersive solvent). Under the optimum conditions, the targets were good linearity in the range of 0.1 µg/L–25 µg/L and the correlation coefficient above 0.9998. The mean recoveries of all analytes were in the ranged from 70.06%–115.65% with RSDs below 8.54%. The detection limits were in the range of 0.001 µg/L–0.01µg/L. The proposed method is a fast and effective sample preparation with good enrichment and extraction efficiency, which can simultaneously detect pesticides and aflatoxins in China herbal tea.http://www.mdpi.com/1420-3049/24/6/1029vortex-assisted dispersive liquid-liquid microextractionChina herbal teapesticides residueaflatoxinsUPLC-MS/MS |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rui Zhang Zhen-Chao Tan Ke-Cheng Huang Yan Wen Xiang-Ying Li Jun-Long Zhao Cheng-Lan Liu |
spellingShingle |
Rui Zhang Zhen-Chao Tan Ke-Cheng Huang Yan Wen Xiang-Ying Li Jun-Long Zhao Cheng-Lan Liu A Vortex-Assisted Dispersive Liquid-Liquid Microextraction Followed by UPLC-MS/MS for Simultaneous Determination of Pesticides and Aflatoxins in Herbal Tea Molecules vortex-assisted dispersive liquid-liquid microextraction China herbal tea pesticides residue aflatoxins UPLC-MS/MS |
author_facet |
Rui Zhang Zhen-Chao Tan Ke-Cheng Huang Yan Wen Xiang-Ying Li Jun-Long Zhao Cheng-Lan Liu |
author_sort |
Rui Zhang |
title |
A Vortex-Assisted Dispersive Liquid-Liquid Microextraction Followed by UPLC-MS/MS for Simultaneous Determination of Pesticides and Aflatoxins in Herbal Tea |
title_short |
A Vortex-Assisted Dispersive Liquid-Liquid Microextraction Followed by UPLC-MS/MS for Simultaneous Determination of Pesticides and Aflatoxins in Herbal Tea |
title_full |
A Vortex-Assisted Dispersive Liquid-Liquid Microextraction Followed by UPLC-MS/MS for Simultaneous Determination of Pesticides and Aflatoxins in Herbal Tea |
title_fullStr |
A Vortex-Assisted Dispersive Liquid-Liquid Microextraction Followed by UPLC-MS/MS for Simultaneous Determination of Pesticides and Aflatoxins in Herbal Tea |
title_full_unstemmed |
A Vortex-Assisted Dispersive Liquid-Liquid Microextraction Followed by UPLC-MS/MS for Simultaneous Determination of Pesticides and Aflatoxins in Herbal Tea |
title_sort |
vortex-assisted dispersive liquid-liquid microextraction followed by uplc-ms/ms for simultaneous determination of pesticides and aflatoxins in herbal tea |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2019-03-01 |
description |
A method for detecting the organophosphorus pesticides residue and aflatoxins in China herbal tea has been developed by UPLC-MS/MS coupled with vortex-assisted dispersive liquid-liquid microextraction (DLLME). The extraction conditions for vortex-assisted DLLME extraction were optimized using single-factor experiments and response surface design. The optimum conditions for the experiment were the pH 5.1, 347 µL of chloroform (extraction solvent) and 1614 µL of acetonitrile (dispersive solvent). Under the optimum conditions, the targets were good linearity in the range of 0.1 µg/L–25 µg/L and the correlation coefficient above 0.9998. The mean recoveries of all analytes were in the ranged from 70.06%–115.65% with RSDs below 8.54%. The detection limits were in the range of 0.001 µg/L–0.01µg/L. The proposed method is a fast and effective sample preparation with good enrichment and extraction efficiency, which can simultaneously detect pesticides and aflatoxins in China herbal tea. |
topic |
vortex-assisted dispersive liquid-liquid microextraction China herbal tea pesticides residue aflatoxins UPLC-MS/MS |
url |
http://www.mdpi.com/1420-3049/24/6/1029 |
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