Molecular characterization of Cryptosporidium xiaoi in goat kids in Bangladesh by nested PCR amplification of 18S rRNA gene
Objective: To investigate the prevalence of Cryptosporidium spp. in goat kids in selected areas of Bangladesh and to elucidate the potential zoonotic hazards. Methods: In the present study, we have used Ziehl-Neelsen staining and nested PCR approach to identify and characterize the Cryptosporidium s...
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Wolters Kluwer Medknow Publications
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doaj-b78e76ce07e446f9ac6816de3f1485d02020-11-24T21:02:06ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912015-03-015320220710.1016/S2221-1691(15)30007-1Molecular characterization of Cryptosporidium xiaoi in goat kids in Bangladesh by nested PCR amplification of 18S rRNA geneAMAM Zonaed Siddiki0Sohana Akter Mina1Zinat Farzana2Bibi Ayesa3Rasel Das4Mohammad Alamgir Hossain5Department of Pathology and Parasitology, Chittagong Veterinary and Animal Sciences University (CVASU), Chittagong-4202, BangladeshDepartment of Genetic Engineering and Biotechnology, University of Chittagong, Chittagong – 4331, BangladeshDepartment of Genetic Engineering and Biotechnology, University of Chittagong, Chittagong – 4331, BangladeshDepartment of Genetic Engineering and Biotechnology, University of Chittagong, Chittagong – 4331, BangladeshNanotechnology and Catalysis Research Center (NANOCEN), University of Malaya, 50603, Kuala Lumpur, MalaysiaDepartment of Pathology and Parasitology, Chittagong Veterinary and Animal Sciences University (CVASU), Chittagong-4202, BangladeshObjective: To investigate the prevalence of Cryptosporidium spp. in goat kids in selected areas of Bangladesh and to elucidate the potential zoonotic hazards. Methods: In the present study, we have used Ziehl-Neelsen staining and nested PCR approach to identify and characterize the Cryptosporidium sp. from diarrhoeic feces of goat kids. A total of 100 diarrhoeic feces samples were collected from Chittagong region in Southern Bangladesh. For nested PCR analysis, specific primers for amplification of 581 base pair fragments of 18S rRNA gene were used. Results: A total of 15% and 3% samples were found positive in microscopic study and in nested PCR analysis respectively. Phylogenetic analysis of sequence data showed similarity with that of Cryptosporidium xiaoi recorded from sheep and goat. Conclusions: To our knowledge, this is the first report of Cryptosporidium xiaoi responsible for diarrhoea in goat kids in Bangladesh. Further study can highlight their zoonotic significance along with genetic diversity in other host species inside the country.http://www.sciencedirect.com/science/article/pii/S2221169115300071CryptosporidiumNested PCRPhylogenetic analysis18S rRNA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
AMAM Zonaed Siddiki Sohana Akter Mina Zinat Farzana Bibi Ayesa Rasel Das Mohammad Alamgir Hossain |
spellingShingle |
AMAM Zonaed Siddiki Sohana Akter Mina Zinat Farzana Bibi Ayesa Rasel Das Mohammad Alamgir Hossain Molecular characterization of Cryptosporidium xiaoi in goat kids in Bangladesh by nested PCR amplification of 18S rRNA gene Asian Pacific Journal of Tropical Biomedicine Cryptosporidium Nested PCR Phylogenetic analysis 18S rRNA |
author_facet |
AMAM Zonaed Siddiki Sohana Akter Mina Zinat Farzana Bibi Ayesa Rasel Das Mohammad Alamgir Hossain |
author_sort |
AMAM Zonaed Siddiki |
title |
Molecular characterization of Cryptosporidium xiaoi in goat kids in Bangladesh by nested PCR amplification of 18S rRNA gene |
title_short |
Molecular characterization of Cryptosporidium xiaoi in goat kids in Bangladesh by nested PCR amplification of 18S rRNA gene |
title_full |
Molecular characterization of Cryptosporidium xiaoi in goat kids in Bangladesh by nested PCR amplification of 18S rRNA gene |
title_fullStr |
Molecular characterization of Cryptosporidium xiaoi in goat kids in Bangladesh by nested PCR amplification of 18S rRNA gene |
title_full_unstemmed |
Molecular characterization of Cryptosporidium xiaoi in goat kids in Bangladesh by nested PCR amplification of 18S rRNA gene |
title_sort |
molecular characterization of cryptosporidium xiaoi in goat kids in bangladesh by nested pcr amplification of 18s rrna gene |
publisher |
Wolters Kluwer Medknow Publications |
series |
Asian Pacific Journal of Tropical Biomedicine |
issn |
2221-1691 |
publishDate |
2015-03-01 |
description |
Objective: To investigate the prevalence of Cryptosporidium spp. in goat kids in selected areas of Bangladesh and to elucidate the potential zoonotic hazards.
Methods: In the present study, we have used Ziehl-Neelsen staining and nested PCR approach to identify and characterize the Cryptosporidium sp. from diarrhoeic feces of goat kids. A total of 100 diarrhoeic feces samples were collected from Chittagong region in Southern Bangladesh. For nested PCR analysis, specific primers for amplification of 581 base pair fragments of 18S rRNA gene were used.
Results: A total of 15% and 3% samples were found positive in microscopic study and in nested PCR analysis respectively. Phylogenetic analysis of sequence data showed similarity with that of Cryptosporidium xiaoi recorded from sheep and goat.
Conclusions: To our knowledge, this is the first report of Cryptosporidium xiaoi responsible for diarrhoea in goat kids in Bangladesh. Further study can highlight their zoonotic significance along with genetic diversity in other host species inside the country. |
topic |
Cryptosporidium Nested PCR Phylogenetic analysis 18S rRNA |
url |
http://www.sciencedirect.com/science/article/pii/S2221169115300071 |
work_keys_str_mv |
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