Isomaltulose Exhibits Prebiotic Activity, and Modulates Gut Microbiota, the Production of Short Chain Fatty Acids, and Secondary Bile Acids in Rats
In vitro experiments have indicated prebiotic activity of isomaltulose, which stimulates the growth of probiotics and the production of short chain fatty acids (SCFAs). However, the absence of in vivo trials undermines these results. This study aims to investigate the effect of isomaltulose on compo...
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doaj-b7bd883e3a4a410393139f888e4660172021-04-23T23:03:05ZengMDPI AGMolecules1420-30492021-04-01262464246410.3390/molecules26092464Isomaltulose Exhibits Prebiotic Activity, and Modulates Gut Microbiota, the Production of Short Chain Fatty Acids, and Secondary Bile Acids in RatsZhan-Dong Yang0Yi-Shan Guo1Jun-Sheng Huang2Ya-Fei Gao3Fei Peng4Ri-Yi Xu5Hui-Hui Su6Ping-Jun Zhang7School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaGuangdong Engineering Lab of High Value Utilization of Biomass, Institute of Bioengineering, Guangdong Academy of Sciences, Guangzhou 510316, ChinaGuangdong Engineering Lab of High Value Utilization of Biomass, Institute of Bioengineering, Guangdong Academy of Sciences, Guangzhou 510316, ChinaGuangdong Engineering Lab of High Value Utilization of Biomass, Institute of Bioengineering, Guangdong Academy of Sciences, Guangzhou 510316, ChinaSchool of Food Science and Engineering, Nanchang University, Nanchang 330000, ChinaGuangdong Engineering Lab of High Value Utilization of Biomass, Institute of Bioengineering, Guangdong Academy of Sciences, Guangzhou 510316, ChinaGuangdong Engineering Lab of High Value Utilization of Biomass, Institute of Bioengineering, Guangdong Academy of Sciences, Guangzhou 510316, ChinaGuangdong Engineering Lab of High Value Utilization of Biomass, Institute of Bioengineering, Guangdong Academy of Sciences, Guangzhou 510316, ChinaIn vitro experiments have indicated prebiotic activity of isomaltulose, which stimulates the growth of probiotics and the production of short chain fatty acids (SCFAs). However, the absence of in vivo trials undermines these results. This study aims to investigate the effect of isomaltulose on composition and functionality of gut microbiota in rats. Twelve Sprague–Dawley rats were divided into two groups: the IsoMTL group was given free access to water containing 10% isomaltulose (<i>w/w</i>), and the control group was treated with normal water for five weeks. Moreover, 16S rRNA sequencing showed that ingestion of isomaltulose increased the abundances of beneficial microbiota, such as <i>Faecalibacterium</i> and <i>Phascolarctobacterium</i>, and decreased levels of pathogens, including <i>Shuttleworthia</i>. Bacterial functional prediction showed that isomaltulose affected gut microbial functionalities, including secondary bile acid biosynthesis. Targeted metabolomics demonstrated that isomaltulose supplementation enhanced cholic acid concentration, and reduced levels of lithocholic acid, deoxycholic acid, dehydrocholic acid, and hyodeoxycholic acid. Moreover, the concentrations of propionate and butyrate were elevated in the rats administered with isomaltulose. This work suggests that isomaltulose modulates gut microbiota and the production of SCFAs and secondary bile acids in rats, which provides a scientific basis on the use of isomaltulose as a prebiotic.https://www.mdpi.com/1420-3049/26/9/2464isomaltuloseprebioticsgut microbiotasecondary bile acidsshort chain fatty acids |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhan-Dong Yang Yi-Shan Guo Jun-Sheng Huang Ya-Fei Gao Fei Peng Ri-Yi Xu Hui-Hui Su Ping-Jun Zhang |
spellingShingle |
Zhan-Dong Yang Yi-Shan Guo Jun-Sheng Huang Ya-Fei Gao Fei Peng Ri-Yi Xu Hui-Hui Su Ping-Jun Zhang Isomaltulose Exhibits Prebiotic Activity, and Modulates Gut Microbiota, the Production of Short Chain Fatty Acids, and Secondary Bile Acids in Rats Molecules isomaltulose prebiotics gut microbiota secondary bile acids short chain fatty acids |
author_facet |
Zhan-Dong Yang Yi-Shan Guo Jun-Sheng Huang Ya-Fei Gao Fei Peng Ri-Yi Xu Hui-Hui Su Ping-Jun Zhang |
author_sort |
Zhan-Dong Yang |
title |
Isomaltulose Exhibits Prebiotic Activity, and Modulates Gut Microbiota, the Production of Short Chain Fatty Acids, and Secondary Bile Acids in Rats |
title_short |
Isomaltulose Exhibits Prebiotic Activity, and Modulates Gut Microbiota, the Production of Short Chain Fatty Acids, and Secondary Bile Acids in Rats |
title_full |
Isomaltulose Exhibits Prebiotic Activity, and Modulates Gut Microbiota, the Production of Short Chain Fatty Acids, and Secondary Bile Acids in Rats |
title_fullStr |
Isomaltulose Exhibits Prebiotic Activity, and Modulates Gut Microbiota, the Production of Short Chain Fatty Acids, and Secondary Bile Acids in Rats |
title_full_unstemmed |
Isomaltulose Exhibits Prebiotic Activity, and Modulates Gut Microbiota, the Production of Short Chain Fatty Acids, and Secondary Bile Acids in Rats |
title_sort |
isomaltulose exhibits prebiotic activity, and modulates gut microbiota, the production of short chain fatty acids, and secondary bile acids in rats |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2021-04-01 |
description |
In vitro experiments have indicated prebiotic activity of isomaltulose, which stimulates the growth of probiotics and the production of short chain fatty acids (SCFAs). However, the absence of in vivo trials undermines these results. This study aims to investigate the effect of isomaltulose on composition and functionality of gut microbiota in rats. Twelve Sprague–Dawley rats were divided into two groups: the IsoMTL group was given free access to water containing 10% isomaltulose (<i>w/w</i>), and the control group was treated with normal water for five weeks. Moreover, 16S rRNA sequencing showed that ingestion of isomaltulose increased the abundances of beneficial microbiota, such as <i>Faecalibacterium</i> and <i>Phascolarctobacterium</i>, and decreased levels of pathogens, including <i>Shuttleworthia</i>. Bacterial functional prediction showed that isomaltulose affected gut microbial functionalities, including secondary bile acid biosynthesis. Targeted metabolomics demonstrated that isomaltulose supplementation enhanced cholic acid concentration, and reduced levels of lithocholic acid, deoxycholic acid, dehydrocholic acid, and hyodeoxycholic acid. Moreover, the concentrations of propionate and butyrate were elevated in the rats administered with isomaltulose. This work suggests that isomaltulose modulates gut microbiota and the production of SCFAs and secondary bile acids in rats, which provides a scientific basis on the use of isomaltulose as a prebiotic. |
topic |
isomaltulose prebiotics gut microbiota secondary bile acids short chain fatty acids |
url |
https://www.mdpi.com/1420-3049/26/9/2464 |
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