Biological Differentiation of Dampness-Heat Syndromes in Chronic Hepatitis B: From Comparative MicroRNA Microarray Profiling to Biomarker Identification
Increasing interest is aroused by traditional Chinese medicine (TCM) treatment of chronic hepatitis B (CHB) based on specific TCM syndrome. As the most common CHB syndromes, spleen-stomach dampness-heat (SSDH) syndrome and liver-gallbladder dampness-heat (LGDH) syndrome are still apt to be confused...
Main Authors: | , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2020-01-01
|
Series: | Evidence-Based Complementary and Alternative Medicine |
Online Access: | http://dx.doi.org/10.1155/2020/7234893 |
id |
doaj-b7cf9a6ea995443985648052b7c6f42d |
---|---|
record_format |
Article |
spelling |
doaj-b7cf9a6ea995443985648052b7c6f42d2020-11-25T02:05:15ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882020-01-01202010.1155/2020/72348937234893Biological Differentiation of Dampness-Heat Syndromes in Chronic Hepatitis B: From Comparative MicroRNA Microarray Profiling to Biomarker IdentificationLi Wen0Cen Jiang1Ting-Jun Wan2Dong Wang3Di Yan4Gui-Yu Li5Yue Su6Xi-Yang Liu7Li-Jun Rong8Hua Ye9Bai-Xue Li10Quan-Sheng Feng11College of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, ChinaCollege of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, ChinaCollege of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, ChinaCollege of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, ChinaCollege of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, ChinaCollege of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, ChinaCollege of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, ChinaCollege of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, ChinaDepartment of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USACollege of Medical Information Engineering, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, ChinaCollege of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, ChinaCollege of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, ChinaIncreasing interest is aroused by traditional Chinese medicine (TCM) treatment of chronic hepatitis B (CHB) based on specific TCM syndrome. As the most common CHB syndromes, spleen-stomach dampness-heat (SSDH) syndrome and liver-gallbladder dampness-heat (LGDH) syndrome are still apt to be confused in TCM diagnosis, greatly hindering the stable exertion of TCM effectiveness. It is urgently needed to provide objective and biological evidences for differentiation and identification of the two significant syndromes. In this study, microRNA (miRNA) microarray analyses coupled with bioinformatics were employed for comparative miRNA profiling of SSDH and LGDH patients. It was found that the two syndromes had both the same and different significantly differentially expressed miRNAs (SDE-miRNAs). Commonness and specificity were also both found between their SDE-miRNA-based bioinformatics analyses, including Hierarchical Clustering, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and miRNA-GO/pathway networks. Furthermore, syndrome-specific SDE-miRNAs were identified as the potential biomarkers, including hsa-miR-1273g-3p and hsa-miR-4419b for SSDH as well as hsa-miR-129-1-3p and hsa-miR-129-2-3p for LGDH. All these laid biological and clinical bases for classification and diagnosis of the two significant CHB dampness-heat syndromes including SSDH and LGDH, providing more opportunities for better application of TCM efficacy and superiority in CHB treatment.http://dx.doi.org/10.1155/2020/7234893 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Li Wen Cen Jiang Ting-Jun Wan Dong Wang Di Yan Gui-Yu Li Yue Su Xi-Yang Liu Li-Jun Rong Hua Ye Bai-Xue Li Quan-Sheng Feng |
spellingShingle |
Li Wen Cen Jiang Ting-Jun Wan Dong Wang Di Yan Gui-Yu Li Yue Su Xi-Yang Liu Li-Jun Rong Hua Ye Bai-Xue Li Quan-Sheng Feng Biological Differentiation of Dampness-Heat Syndromes in Chronic Hepatitis B: From Comparative MicroRNA Microarray Profiling to Biomarker Identification Evidence-Based Complementary and Alternative Medicine |
author_facet |
Li Wen Cen Jiang Ting-Jun Wan Dong Wang Di Yan Gui-Yu Li Yue Su Xi-Yang Liu Li-Jun Rong Hua Ye Bai-Xue Li Quan-Sheng Feng |
author_sort |
Li Wen |
title |
Biological Differentiation of Dampness-Heat Syndromes in Chronic Hepatitis B: From Comparative MicroRNA Microarray Profiling to Biomarker Identification |
title_short |
Biological Differentiation of Dampness-Heat Syndromes in Chronic Hepatitis B: From Comparative MicroRNA Microarray Profiling to Biomarker Identification |
title_full |
Biological Differentiation of Dampness-Heat Syndromes in Chronic Hepatitis B: From Comparative MicroRNA Microarray Profiling to Biomarker Identification |
title_fullStr |
Biological Differentiation of Dampness-Heat Syndromes in Chronic Hepatitis B: From Comparative MicroRNA Microarray Profiling to Biomarker Identification |
title_full_unstemmed |
Biological Differentiation of Dampness-Heat Syndromes in Chronic Hepatitis B: From Comparative MicroRNA Microarray Profiling to Biomarker Identification |
title_sort |
biological differentiation of dampness-heat syndromes in chronic hepatitis b: from comparative microrna microarray profiling to biomarker identification |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2020-01-01 |
description |
Increasing interest is aroused by traditional Chinese medicine (TCM) treatment of chronic hepatitis B (CHB) based on specific TCM syndrome. As the most common CHB syndromes, spleen-stomach dampness-heat (SSDH) syndrome and liver-gallbladder dampness-heat (LGDH) syndrome are still apt to be confused in TCM diagnosis, greatly hindering the stable exertion of TCM effectiveness. It is urgently needed to provide objective and biological evidences for differentiation and identification of the two significant syndromes. In this study, microRNA (miRNA) microarray analyses coupled with bioinformatics were employed for comparative miRNA profiling of SSDH and LGDH patients. It was found that the two syndromes had both the same and different significantly differentially expressed miRNAs (SDE-miRNAs). Commonness and specificity were also both found between their SDE-miRNA-based bioinformatics analyses, including Hierarchical Clustering, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and miRNA-GO/pathway networks. Furthermore, syndrome-specific SDE-miRNAs were identified as the potential biomarkers, including hsa-miR-1273g-3p and hsa-miR-4419b for SSDH as well as hsa-miR-129-1-3p and hsa-miR-129-2-3p for LGDH. All these laid biological and clinical bases for classification and diagnosis of the two significant CHB dampness-heat syndromes including SSDH and LGDH, providing more opportunities for better application of TCM efficacy and superiority in CHB treatment. |
url |
http://dx.doi.org/10.1155/2020/7234893 |
work_keys_str_mv |
AT liwen biologicaldifferentiationofdampnessheatsyndromesinchronichepatitisbfromcomparativemicrornamicroarrayprofilingtobiomarkeridentification AT cenjiang biologicaldifferentiationofdampnessheatsyndromesinchronichepatitisbfromcomparativemicrornamicroarrayprofilingtobiomarkeridentification AT tingjunwan biologicaldifferentiationofdampnessheatsyndromesinchronichepatitisbfromcomparativemicrornamicroarrayprofilingtobiomarkeridentification AT dongwang biologicaldifferentiationofdampnessheatsyndromesinchronichepatitisbfromcomparativemicrornamicroarrayprofilingtobiomarkeridentification AT diyan biologicaldifferentiationofdampnessheatsyndromesinchronichepatitisbfromcomparativemicrornamicroarrayprofilingtobiomarkeridentification AT guiyuli biologicaldifferentiationofdampnessheatsyndromesinchronichepatitisbfromcomparativemicrornamicroarrayprofilingtobiomarkeridentification AT yuesu biologicaldifferentiationofdampnessheatsyndromesinchronichepatitisbfromcomparativemicrornamicroarrayprofilingtobiomarkeridentification AT xiyangliu biologicaldifferentiationofdampnessheatsyndromesinchronichepatitisbfromcomparativemicrornamicroarrayprofilingtobiomarkeridentification AT lijunrong biologicaldifferentiationofdampnessheatsyndromesinchronichepatitisbfromcomparativemicrornamicroarrayprofilingtobiomarkeridentification AT huaye biologicaldifferentiationofdampnessheatsyndromesinchronichepatitisbfromcomparativemicrornamicroarrayprofilingtobiomarkeridentification AT baixueli biologicaldifferentiationofdampnessheatsyndromesinchronichepatitisbfromcomparativemicrornamicroarrayprofilingtobiomarkeridentification AT quanshengfeng biologicaldifferentiationofdampnessheatsyndromesinchronichepatitisbfromcomparativemicrornamicroarrayprofilingtobiomarkeridentification |
_version_ |
1715577155909320704 |