Identification of Nocardia Species Isolated from Soil Samples of the City of Tehran, Iran, Using Phenotypic Tests
Background: Nocardia are Gram-positive, aerobic and partially acid-fast and cause dangerous infectious disease, nocardiosis, by entering the respiratory tract or damaged skin. In this study, we used various biochemical tests for identificating Nocardia species in soil samples of the city of Tehran,...
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doaj-b7d8e67429654576a77412e805a34c7f2020-11-24T22:04:45ZfasVesnu Publications مجله دانشکده پزشکی اصفهان1027-75951735-854X2014-02-0131265208120881484Identification of Nocardia Species Isolated from Soil Samples of the City of Tehran, Iran, Using Phenotypic TestsMasoumeh Rasouli-nasab0Shadi Habibnia1Parvin Heidarieh2Mehdi Fatahi-Bafghi3Mohammad Reza Pourmand4Seyyed Saeed Eshraghi5Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranAssistant Professor, Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, IranPhD Candidate, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranAssociate Professor, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranProfessor, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranBackground: Nocardia are Gram-positive, aerobic and partially acid-fast and cause dangerous infectious disease, nocardiosis, by entering the respiratory tract or damaged skin. In this study, we used various biochemical tests for identificating Nocardia species in soil samples of the city of Tehran, Iran. Methods: After isolation of Nocardia using paraffin baiting method, for detection and identification of various species, some phenotypic tests including growth in lysozyme broth, hydrolysis of amino acids such as hypoxanthine, xanthine, tyrosine, casein, gelatin and urea, production of nitrate reductase, growth at 35 °C and 45 °C, and utilization of some carbohydrates were used. Findings: The analysis of phenotypic tests presented that 12 isolates of Nocardia asteroides (63.15%), 5 isolates of Nocardia cyriacigeorgica (26.31%), and 1 isolate of Nocardia otitidiscaviarum (5.26%) were identified. Only 1 isolate (5.26%) remained undetected after phenotypic tests. Conclusion: In this study, several Nocardia species isolated from the soil samples of Tehran which were phenotypically similar to the well-known species. However, further identification tests are required to confirm the heterogeneity of the isolates and to identify the exact phenotype and genotype of the Nocardia species.http://jims.mui.ac.ir/index.php/jims/article/view/3126NocardiaSoilPhenotypic testIran |
collection |
DOAJ |
language |
fas |
format |
Article |
sources |
DOAJ |
author |
Masoumeh Rasouli-nasab Shadi Habibnia Parvin Heidarieh Mehdi Fatahi-Bafghi Mohammad Reza Pourmand Seyyed Saeed Eshraghi |
spellingShingle |
Masoumeh Rasouli-nasab Shadi Habibnia Parvin Heidarieh Mehdi Fatahi-Bafghi Mohammad Reza Pourmand Seyyed Saeed Eshraghi Identification of Nocardia Species Isolated from Soil Samples of the City of Tehran, Iran, Using Phenotypic Tests مجله دانشکده پزشکی اصفهان Nocardia Soil Phenotypic test Iran |
author_facet |
Masoumeh Rasouli-nasab Shadi Habibnia Parvin Heidarieh Mehdi Fatahi-Bafghi Mohammad Reza Pourmand Seyyed Saeed Eshraghi |
author_sort |
Masoumeh Rasouli-nasab |
title |
Identification of Nocardia Species Isolated from Soil Samples of the City of Tehran, Iran, Using Phenotypic Tests |
title_short |
Identification of Nocardia Species Isolated from Soil Samples of the City of Tehran, Iran, Using Phenotypic Tests |
title_full |
Identification of Nocardia Species Isolated from Soil Samples of the City of Tehran, Iran, Using Phenotypic Tests |
title_fullStr |
Identification of Nocardia Species Isolated from Soil Samples of the City of Tehran, Iran, Using Phenotypic Tests |
title_full_unstemmed |
Identification of Nocardia Species Isolated from Soil Samples of the City of Tehran, Iran, Using Phenotypic Tests |
title_sort |
identification of nocardia species isolated from soil samples of the city of tehran, iran, using phenotypic tests |
publisher |
Vesnu Publications |
series |
مجله دانشکده پزشکی اصفهان |
issn |
1027-7595 1735-854X |
publishDate |
2014-02-01 |
description |
Background: Nocardia are Gram-positive, aerobic and partially acid-fast and cause dangerous infectious disease, nocardiosis, by entering the respiratory tract or damaged skin. In this study, we used various biochemical tests for identificating Nocardia species in soil samples of the city of Tehran, Iran.
Methods: After isolation of Nocardia using paraffin baiting method, for detection and identification of various species, some phenotypic tests including growth in lysozyme broth, hydrolysis of amino acids such as hypoxanthine, xanthine, tyrosine, casein, gelatin and urea, production of nitrate reductase, growth at 35 °C and 45 °C, and utilization of some carbohydrates were used.
Findings: The analysis of phenotypic tests presented that 12 isolates of Nocardia asteroides (63.15%), 5 isolates of Nocardia cyriacigeorgica (26.31%), and 1 isolate of Nocardia otitidiscaviarum (5.26%) were identified. Only 1 isolate (5.26%) remained undetected after phenotypic tests.
Conclusion: In this study, several Nocardia species isolated from the soil samples of Tehran which were phenotypically similar to the well-known species. However, further identification tests are required to confirm the heterogeneity of the isolates and to identify the exact phenotype and genotype of the Nocardia species. |
topic |
Nocardia Soil Phenotypic test Iran |
url |
http://jims.mui.ac.ir/index.php/jims/article/view/3126 |
work_keys_str_mv |
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