Characterisation of a cysteine protease from poultry red mites and its potential use as a vaccine for chickens
Poultry red mites (PRMs, Dermanyssus gallinae) are ectoparasites that negatively affect farmed chickens, leading to serious economic losses worldwide. Acaricides have been used to control PRMs in poultry houses. However, some PRMs have developed resistance to acaricides, and therefore different appr...
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doaj-b7ecd80f77324f04ad1ddaf9f769ce542021-02-18T10:47:41ZengEDP SciencesParasite1776-10422021-01-0128910.1051/parasite/2021005parasite200142Characterisation of a cysteine protease from poultry red mites and its potential use as a vaccine for chickensMurata Shirohttps://orcid.org/0000-0002-2569-899XTaniguchi Ayaka0Isezaki Masayoshi1Fujisawa Sotaro2Sakai Eishi3Taneno Akira4Ichii Osamu5Ito Takuya6Maekawa Naoya7Okagawa Tomohiro8Konnai SatoruOhashi KazuhikoDepartment of Disease Control, Faculty of Veterinary Medicine, Hokkaido UniversityDepartment of Disease Control, Faculty of Veterinary Medicine, Hokkaido UniversityDepartment of Disease Control, Faculty of Veterinary Medicine, Hokkaido UniversityVaxxinova Japan K.K.Vaxxinova Japan K.K.Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido UniversityHokkaido Institute of Public HealthDepartment of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido UniversityDepartment of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido UniversityPoultry red mites (PRMs, Dermanyssus gallinae) are ectoparasites that negatively affect farmed chickens, leading to serious economic losses worldwide. Acaricides have been used to control PRMs in poultry houses. However, some PRMs have developed resistance to acaricides, and therefore different approaches are required to manage the problems caused by PRMs. Vaccination of chickens is one of the methods being considered to reduce the number of PRMs in poultry houses. In a previous study, a cysteine protease, Deg-CPR-1, was identified as a candidate vaccine against PRMs distributed in Europe. In this study, we investigated the characteristics of Deg-CPR-1. A phylogenetic analysis revealed that Deg-CPR-1 is closely related to the digestive cysteine proteases of other mite species, and it was classified into a cluster different from that of chicken cathepsins. Deg-CPR-1 of PRMs in Japan has an amino acid substitution compared with that of PRMs in Europe, but it showed efficacy as a vaccine, consistent with previous findings. Deg-CPR-1 exhibited cathepsin L-like enzyme activity. In addition, the Deg-CPR-1 mRNA was expressed in the midgut and in all stages of PRMs that feed on blood. These results imply that Deg-CPR-1 in the midgut may have important functions in physiological processes, and the inhibition of its expression may contribute to the efficacy of a Deg-CPR-1-based vaccine. Further research is required to fully understand the mechanisms of vaccine efficacy.https://www.parasite-journal.org/articles/parasite/full_html/2021/01/parasite200142/parasite200142.htmlpoultry red mitecysteine proteasecathepsin lvaccine candidatedeg-cpr-1dermanyssus gallinae |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Murata Shiro Taniguchi Ayaka Isezaki Masayoshi Fujisawa Sotaro Sakai Eishi Taneno Akira Ichii Osamu Ito Takuya Maekawa Naoya Okagawa Tomohiro Konnai Satoru Ohashi Kazuhiko |
spellingShingle |
Murata Shiro Taniguchi Ayaka Isezaki Masayoshi Fujisawa Sotaro Sakai Eishi Taneno Akira Ichii Osamu Ito Takuya Maekawa Naoya Okagawa Tomohiro Konnai Satoru Ohashi Kazuhiko Characterisation of a cysteine protease from poultry red mites and its potential use as a vaccine for chickens Parasite poultry red mite cysteine protease cathepsin l vaccine candidate deg-cpr-1 dermanyssus gallinae |
author_facet |
Murata Shiro Taniguchi Ayaka Isezaki Masayoshi Fujisawa Sotaro Sakai Eishi Taneno Akira Ichii Osamu Ito Takuya Maekawa Naoya Okagawa Tomohiro Konnai Satoru Ohashi Kazuhiko |
author_sort |
Murata Shiro |
title |
Characterisation of a cysteine protease from poultry red mites and its potential use as a vaccine for chickens |
title_short |
Characterisation of a cysteine protease from poultry red mites and its potential use as a vaccine for chickens |
title_full |
Characterisation of a cysteine protease from poultry red mites and its potential use as a vaccine for chickens |
title_fullStr |
Characterisation of a cysteine protease from poultry red mites and its potential use as a vaccine for chickens |
title_full_unstemmed |
Characterisation of a cysteine protease from poultry red mites and its potential use as a vaccine for chickens |
title_sort |
characterisation of a cysteine protease from poultry red mites and its potential use as a vaccine for chickens |
publisher |
EDP Sciences |
series |
Parasite |
issn |
1776-1042 |
publishDate |
2021-01-01 |
description |
Poultry red mites (PRMs, Dermanyssus gallinae) are ectoparasites that negatively affect farmed chickens, leading to serious economic losses worldwide. Acaricides have been used to control PRMs in poultry houses. However, some PRMs have developed resistance to acaricides, and therefore different approaches are required to manage the problems caused by PRMs. Vaccination of chickens is one of the methods being considered to reduce the number of PRMs in poultry houses. In a previous study, a cysteine protease, Deg-CPR-1, was identified as a candidate vaccine against PRMs distributed in Europe. In this study, we investigated the characteristics of Deg-CPR-1. A phylogenetic analysis revealed that Deg-CPR-1 is closely related to the digestive cysteine proteases of other mite species, and it was classified into a cluster different from that of chicken cathepsins. Deg-CPR-1 of PRMs in Japan has an amino acid substitution compared with that of PRMs in Europe, but it showed efficacy as a vaccine, consistent with previous findings. Deg-CPR-1 exhibited cathepsin L-like enzyme activity. In addition, the Deg-CPR-1 mRNA was expressed in the midgut and in all stages of PRMs that feed on blood. These results imply that Deg-CPR-1 in the midgut may have important functions in physiological processes, and the inhibition of its expression may contribute to the efficacy of a Deg-CPR-1-based vaccine. Further research is required to fully understand the mechanisms of vaccine efficacy. |
topic |
poultry red mite cysteine protease cathepsin l vaccine candidate deg-cpr-1 dermanyssus gallinae |
url |
https://www.parasite-journal.org/articles/parasite/full_html/2021/01/parasite200142/parasite200142.html |
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