LncRNA HOTAIR suppresses cell apoptosis, autophagy and induces cell proliferation in cholangiocarcinoma by modulating the miR-204-5p/HMGB1 axis
Background: Cholangiocarcinoma (CCA) is a malignant tumor in the world. LncRNA HOX transcript antisense intergenic RNA (HOTAIR) was identified as a crucial regulator in various cancers including CCA. This study aimed to unravel the functions of HOTAIR and its biological mechanism in CCA, hinting for...
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doaj-b7ecfca8d6114e2eaa8f88e737b797092021-05-20T07:43:20ZengElsevierBiomedicine & Pharmacotherapy0753-33222020-10-01130110566LncRNA HOTAIR suppresses cell apoptosis, autophagy and induces cell proliferation in cholangiocarcinoma by modulating the miR-204-5p/HMGB1 axisMin Lu0Xinglei Qin1Yajun Zhou2Gang Li3Zhaoyang Liu4Haodi Yue5Xiwen Geng6Department of Cardiology, Henan Provincial People’s Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, School of Clinical Medicine of Henan University, Zhengzhou 450003, Henan, ChinaDepartment of Hepatobiliary Surgery, Henan Provincial People’s Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, School of Clinical Medicine of Henan University, Zhengzhou 450003, Henan, China; Corresponding author at: Department of Hepatobiliary Surgery, Henan Provincial People’s Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, School of Clinical Medicine of Henan University, No.7, Weiwu Road, Zhengzhou 450003, Henan, China.Department of Hepatobiliary Surgery, Henan Provincial People’s Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, School of Clinical Medicine of Henan University, Zhengzhou 450003, Henan, ChinaDepartment of Hepatobiliary Surgery, Henan Provincial People’s Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, School of Clinical Medicine of Henan University, Zhengzhou 450003, Henan, ChinaDepartment of Hepatobiliary Surgery, Henan Provincial People’s Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, School of Clinical Medicine of Henan University, Zhengzhou 450003, Henan, ChinaCenter for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, School of Clinical Medicine of Henan University, Zhengzhou 450003, Henan, ChinaCenter for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, School of Clinical Medicine of Henan University, Zhengzhou 450003, Henan, ChinaBackground: Cholangiocarcinoma (CCA) is a malignant tumor in the world. LncRNA HOX transcript antisense intergenic RNA (HOTAIR) was identified as a crucial regulator in various cancers including CCA. This study aimed to unravel the functions of HOTAIR and its biological mechanism in CCA, hinting for the new therapeutic targets in CCA. Methods: The levels of HOTAIR, miR-204-5p and HMGB1 in CCA tissues and cell lines (HuB28 and HuCCT1) were measured by quantitative real-time polymerase chain reaction (qRT-PCR). Western blot was conducted to detect the protein levels of LC3-I, LC3-II, Beclin-1 and HMGB1. The relationships among HOTAIR, miR-204-5p and HMGB1 were examined by dual-luciferase reporter assay, RNA immunoprecipitation (RIP) assay and RNA pull down assay. Cell proliferation ability and apoptosis rate were assessed by CCK8 assay and flow cytometry, respectively. in vivo experiment was conducted to examine the bio-functions of HOTAIR in nude mice. Results: HOTAIR and HMGB1 were over-expressed, while miR-204-5p was lowly expressed in CCA tissues and cells. The dual-luciferase reporter assay indicated that miR-204-5p was a target of HOTAIR, and HMGB1 was a target of miR-204-5p. The restoration experiments showed that HOTAIR repressed cell apoptosis, autophagy and promoted cell proliferation via miR-204-5p/HMGB1 axis. Additionally, HOTAIR silencing retarded the xenograft tumor growth by up-regulation of miR-204-5p and down-regulation of HMGB1. Conclusion: These data unraveled that lncRNA HOTAIR regulated HMGB1 to suppress cell apoptosis, autophagy and induce cell proliferation by sponging miR-204-5p in CCA. Thus, this new regulatory pathway may provide new therapeutic targets for CCA.http://www.sciencedirect.com/science/article/pii/S0753332220307599HOTAIRmiR-204-5pHMGB1Cholangiocarcinoma |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Min Lu Xinglei Qin Yajun Zhou Gang Li Zhaoyang Liu Haodi Yue Xiwen Geng |
spellingShingle |
Min Lu Xinglei Qin Yajun Zhou Gang Li Zhaoyang Liu Haodi Yue Xiwen Geng LncRNA HOTAIR suppresses cell apoptosis, autophagy and induces cell proliferation in cholangiocarcinoma by modulating the miR-204-5p/HMGB1 axis Biomedicine & Pharmacotherapy HOTAIR miR-204-5p HMGB1 Cholangiocarcinoma |
author_facet |
Min Lu Xinglei Qin Yajun Zhou Gang Li Zhaoyang Liu Haodi Yue Xiwen Geng |
author_sort |
Min Lu |
title |
LncRNA HOTAIR suppresses cell apoptosis, autophagy and induces cell proliferation in cholangiocarcinoma by modulating the miR-204-5p/HMGB1 axis |
title_short |
LncRNA HOTAIR suppresses cell apoptosis, autophagy and induces cell proliferation in cholangiocarcinoma by modulating the miR-204-5p/HMGB1 axis |
title_full |
LncRNA HOTAIR suppresses cell apoptosis, autophagy and induces cell proliferation in cholangiocarcinoma by modulating the miR-204-5p/HMGB1 axis |
title_fullStr |
LncRNA HOTAIR suppresses cell apoptosis, autophagy and induces cell proliferation in cholangiocarcinoma by modulating the miR-204-5p/HMGB1 axis |
title_full_unstemmed |
LncRNA HOTAIR suppresses cell apoptosis, autophagy and induces cell proliferation in cholangiocarcinoma by modulating the miR-204-5p/HMGB1 axis |
title_sort |
lncrna hotair suppresses cell apoptosis, autophagy and induces cell proliferation in cholangiocarcinoma by modulating the mir-204-5p/hmgb1 axis |
publisher |
Elsevier |
series |
Biomedicine & Pharmacotherapy |
issn |
0753-3322 |
publishDate |
2020-10-01 |
description |
Background: Cholangiocarcinoma (CCA) is a malignant tumor in the world. LncRNA HOX transcript antisense intergenic RNA (HOTAIR) was identified as a crucial regulator in various cancers including CCA. This study aimed to unravel the functions of HOTAIR and its biological mechanism in CCA, hinting for the new therapeutic targets in CCA. Methods: The levels of HOTAIR, miR-204-5p and HMGB1 in CCA tissues and cell lines (HuB28 and HuCCT1) were measured by quantitative real-time polymerase chain reaction (qRT-PCR). Western blot was conducted to detect the protein levels of LC3-I, LC3-II, Beclin-1 and HMGB1. The relationships among HOTAIR, miR-204-5p and HMGB1 were examined by dual-luciferase reporter assay, RNA immunoprecipitation (RIP) assay and RNA pull down assay. Cell proliferation ability and apoptosis rate were assessed by CCK8 assay and flow cytometry, respectively. in vivo experiment was conducted to examine the bio-functions of HOTAIR in nude mice. Results: HOTAIR and HMGB1 were over-expressed, while miR-204-5p was lowly expressed in CCA tissues and cells. The dual-luciferase reporter assay indicated that miR-204-5p was a target of HOTAIR, and HMGB1 was a target of miR-204-5p. The restoration experiments showed that HOTAIR repressed cell apoptosis, autophagy and promoted cell proliferation via miR-204-5p/HMGB1 axis. Additionally, HOTAIR silencing retarded the xenograft tumor growth by up-regulation of miR-204-5p and down-regulation of HMGB1. Conclusion: These data unraveled that lncRNA HOTAIR regulated HMGB1 to suppress cell apoptosis, autophagy and induce cell proliferation by sponging miR-204-5p in CCA. Thus, this new regulatory pathway may provide new therapeutic targets for CCA. |
topic |
HOTAIR miR-204-5p HMGB1 Cholangiocarcinoma |
url |
http://www.sciencedirect.com/science/article/pii/S0753332220307599 |
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