Label-Free and Redox Markers-Based Electrochemical Aptasensors for Aflatoxin M1 Detection

• Main Authors: Stefanos Karapetis, Dimitrios Nikolelis, Tibor Hianik
• Format: Article
• Language: English
• Published: MDPI AG 2018-12-01
• Series: Sensors
• Subjects: biosensors; aflatoxin M1; aptasensors; electrochemical impedance spectroscopy; differential pulse voltammetry
• Online Access: https://www.mdpi.com/1424-8220/18/12/4218

We performed a comparative analysis of the sensitivity of aptamer-based biosensors for detection mycotoxin aflatoxin M₁ (AFM1) depending on the method of immobilization of DNA aptamers and method of the detection. Label-free electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) for ferrocene labeled neutravidin layers were used for this purpose. Amino-modified DNA aptamers have been immobilized at the surface of polyamidoamine dendrimers (PAMAM) of fourth generation (G4) or biotin-modified aptamers were immobilized at the neutravidin layer chemisorbed at gold surface. In the first case the limit of detection (LOD) has been determined as 8.47 ng/L. In the second approach the LOD was similar 8.62 ng/L, which is below of allowable limits of AFM1 in milk and milk products. The aptasensors were validated in a spiked milk samples with good recovery better than 78%. Comparative analysis of the sensitivity of immuno- and aptasensors was also performed and showed comparable sensitivity.