Native protein delivery into rice callus using ionic complexes of protein and cell-penetrating peptides.
Direct protein delivery into intact plants remains a challenge for the agricultural and plant science fields. Cell-penetrating peptide (CPP)-mediated protein delivery requires the binding of CPPs to a protein to carry the protein into the cell through the cell wall and lipid bilayer. Thus, we prepar...
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doaj-b7f879ad0d724d94915889e74fc2b16b2021-03-03T20:33:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01147e021403310.1371/journal.pone.0214033Native protein delivery into rice callus using ionic complexes of protein and cell-penetrating peptides.Boyang GuoJun ItamiKazusato OikawaYoko MotodaTakanori KigawaKeiji NumataDirect protein delivery into intact plants remains a challenge for the agricultural and plant science fields. Cell-penetrating peptide (CPP)-mediated protein delivery requires the binding of CPPs to a protein to carry the protein into the cell through the cell wall and lipid bilayer. Thus, we prepared ionic complexes of a CPP-containing carrier peptide and a cargo protein, namely, Citrine yellow fluorescent protein, and subsequently studied their physicochemical properties. Two types of carrier peptides, BP100(KH)9 and BP100CH7, were investigated for delivery efficiency into rice callus. Both BP100(KH)9 and BP100CH7 successfully introduced Citrine protein into rice callus cells under pressure and vacuum treatment. Moreover, delivery efficiency varied at different growth stages of rice callus; 5-day rice callus was a more efficient recipient for Citrine than 21-day callus.https://doi.org/10.1371/journal.pone.0214033 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Boyang Guo Jun Itami Kazusato Oikawa Yoko Motoda Takanori Kigawa Keiji Numata |
spellingShingle |
Boyang Guo Jun Itami Kazusato Oikawa Yoko Motoda Takanori Kigawa Keiji Numata Native protein delivery into rice callus using ionic complexes of protein and cell-penetrating peptides. PLoS ONE |
author_facet |
Boyang Guo Jun Itami Kazusato Oikawa Yoko Motoda Takanori Kigawa Keiji Numata |
author_sort |
Boyang Guo |
title |
Native protein delivery into rice callus using ionic complexes of protein and cell-penetrating peptides. |
title_short |
Native protein delivery into rice callus using ionic complexes of protein and cell-penetrating peptides. |
title_full |
Native protein delivery into rice callus using ionic complexes of protein and cell-penetrating peptides. |
title_fullStr |
Native protein delivery into rice callus using ionic complexes of protein and cell-penetrating peptides. |
title_full_unstemmed |
Native protein delivery into rice callus using ionic complexes of protein and cell-penetrating peptides. |
title_sort |
native protein delivery into rice callus using ionic complexes of protein and cell-penetrating peptides. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2019-01-01 |
description |
Direct protein delivery into intact plants remains a challenge for the agricultural and plant science fields. Cell-penetrating peptide (CPP)-mediated protein delivery requires the binding of CPPs to a protein to carry the protein into the cell through the cell wall and lipid bilayer. Thus, we prepared ionic complexes of a CPP-containing carrier peptide and a cargo protein, namely, Citrine yellow fluorescent protein, and subsequently studied their physicochemical properties. Two types of carrier peptides, BP100(KH)9 and BP100CH7, were investigated for delivery efficiency into rice callus. Both BP100(KH)9 and BP100CH7 successfully introduced Citrine protein into rice callus cells under pressure and vacuum treatment. Moreover, delivery efficiency varied at different growth stages of rice callus; 5-day rice callus was a more efficient recipient for Citrine than 21-day callus. |
url |
https://doi.org/10.1371/journal.pone.0214033 |
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