Umbilical Cord Blood Platelet Lysate as Serum Substitute in Expansion of Human Mesenchymal Stem Cells
Objective: The diverse clinical applications for human mesenchymal stem cells (hMSCs) in cellular therapy and regenerative medicine warrant increased focus on developing adequate culture supplements devoid of animal-derived products. In the present study, we have investigated the feasibility of u...
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doaj-b7fa1e80ed3944b18f9d05ab28f4636f2020-11-25T03:23:15ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142017-08-0119340341410.22074/cellj.2017.4886Umbilical Cord Blood Platelet Lysate as Serum Substitute in Expansion of Human Mesenchymal Stem CellsNegin Shirzad0Sima Bordbar1Alireza Goodarzi2Monire Mohammad3Pardis Khosravani4Froughazam Sayahpour5Mohamadreza Baghaban Eslaminejad6Marzieh Ebrahimi7Department of Regenerative Biomedicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranDepartment of Regenerative Biomedicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranDepartment of Regenerative Biomedicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranDepartment of Regenerative Biomedicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranObjective: The diverse clinical applications for human mesenchymal stem cells (hMSCs) in cellular therapy and regenerative medicine warrant increased focus on developing adequate culture supplements devoid of animal-derived products. In the present study, we have investigated the feasibility of umbilical cord blood-platelet lysate (UCB-PL) as a standard substitute for fetal bovine serum (FBS) and human peripheral blood-PL (PB-PL). Materials and Methods: In this experimental study, platelet concentrates (PC) from UCB and human PB donors were frozen, melted, and sterilized to obtain PL. Quality control included platelet cell counts, sterility testing (viral and microbial), total protein concentrations, growth factor levels, and PL stability. The effects of UCB-PL and PB-PL on hMSCs proliferation and differentiation into osteocytes, chondrocytes, and adipocytes were studied and the results compared with FBS. Results: UCB-PL contained high levels of protein content, platelet-derived growth factor- AB (PDGF-AB), and transforming growth factor (TGF) compared to PB-PL. All growth factors were stable for at least nine months post-storage at -70˚C. hMSCs proliferation enhanced following treatment with UCB-PL. With all three supplements, hMSCs could differentiate into all three lineages. Conclusion: PB-PL and UCB-PL both were potent in hMSCs proliferation. However, PB promoted osteoblastic differentiation and UCB-PL induced chondrogenic differentiation. Because of availability, ease of use and feasible standardization of UCB-PL, we have suggested that UCB-PL be used as an alternative to FBS and PB-PL for the cultivation and expansion of hMSCs in cellular therapy.http://celljournal.org/journal/article/14345/downloadHuman Platelet LysatesUmbilical Cord BloodPeripheral BloodMesenchymal Stem CellGrowth Factor |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Negin Shirzad Sima Bordbar Alireza Goodarzi Monire Mohammad Pardis Khosravani Froughazam Sayahpour Mohamadreza Baghaban Eslaminejad Marzieh Ebrahimi |
spellingShingle |
Negin Shirzad Sima Bordbar Alireza Goodarzi Monire Mohammad Pardis Khosravani Froughazam Sayahpour Mohamadreza Baghaban Eslaminejad Marzieh Ebrahimi Umbilical Cord Blood Platelet Lysate as Serum Substitute in Expansion of Human Mesenchymal Stem Cells Cell Journal Human Platelet Lysates Umbilical Cord Blood Peripheral Blood Mesenchymal Stem Cell Growth Factor |
author_facet |
Negin Shirzad Sima Bordbar Alireza Goodarzi Monire Mohammad Pardis Khosravani Froughazam Sayahpour Mohamadreza Baghaban Eslaminejad Marzieh Ebrahimi |
author_sort |
Negin Shirzad |
title |
Umbilical Cord Blood Platelet Lysate as Serum Substitute in Expansion of Human Mesenchymal Stem Cells |
title_short |
Umbilical Cord Blood Platelet Lysate as Serum Substitute in Expansion of Human Mesenchymal Stem Cells |
title_full |
Umbilical Cord Blood Platelet Lysate as Serum Substitute in Expansion of Human Mesenchymal Stem Cells |
title_fullStr |
Umbilical Cord Blood Platelet Lysate as Serum Substitute in Expansion of Human Mesenchymal Stem Cells |
title_full_unstemmed |
Umbilical Cord Blood Platelet Lysate as Serum Substitute in Expansion of Human Mesenchymal Stem Cells |
title_sort |
umbilical cord blood platelet lysate as serum substitute in expansion of human mesenchymal stem cells |
publisher |
Royan Institute (ACECR), Tehran |
series |
Cell Journal |
issn |
2228-5806 2228-5814 |
publishDate |
2017-08-01 |
description |
Objective: The diverse clinical applications for human mesenchymal stem cells (hMSCs)
in cellular therapy and regenerative medicine warrant increased focus on developing
adequate culture supplements devoid of animal-derived products. In the
present study, we have investigated the feasibility of umbilical cord blood-platelet
lysate (UCB-PL) as a standard substitute for fetal bovine serum (FBS) and human
peripheral blood-PL (PB-PL).
Materials and Methods: In this experimental study, platelet concentrates (PC) from UCB
and human PB donors were frozen, melted, and sterilized to obtain PL. Quality control
included platelet cell counts, sterility testing (viral and microbial), total protein concentrations,
growth factor levels, and PL stability. The effects of UCB-PL and PB-PL on hMSCs
proliferation and differentiation into osteocytes, chondrocytes, and adipocytes were studied
and the results compared with FBS.
Results: UCB-PL contained high levels of protein content, platelet-derived growth factor-
AB (PDGF-AB), and transforming growth factor (TGF) compared to PB-PL. All growth
factors were stable for at least nine months post-storage at -70˚C. hMSCs proliferation
enhanced following treatment with UCB-PL. With all three supplements, hMSCs could
differentiate into all three lineages.
Conclusion: PB-PL and UCB-PL both were potent in hMSCs proliferation. However, PB
promoted osteoblastic differentiation and UCB-PL induced chondrogenic differentiation.
Because of availability, ease of use and feasible standardization of UCB-PL, we have suggested
that UCB-PL be used as an alternative to FBS and PB-PL for the cultivation and
expansion of hMSCs in cellular therapy. |
topic |
Human Platelet Lysates Umbilical Cord Blood Peripheral Blood Mesenchymal Stem Cell Growth Factor |
url |
http://celljournal.org/journal/article/14345/download |
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