Application of xCELLigence RTCA Biosensor Technology for Revealing the Profile and Window of Drug Responsiveness in Real Time
The xCELLigence technology is a real-time cellular biosensor, which measures the net adhesion of cells to high-density gold electrode arrays printed on custom-designed E-plates. The strength of cellular adhesion is influenced by a myriad of factors that include cell type, cell viability, growth, mig...
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doaj-b81383ef459d4a4d9e8322f12808f8c02020-11-24T23:30:45ZengMDPI AGBiosensors2079-63742015-04-015219922210.3390/bios5020199bios5020199Application of xCELLigence RTCA Biosensor Technology for Revealing the Profile and Window of Drug Responsiveness in Real TimeDan Kho0Christa MacDonald1Rebecca Johnson2Charles P. Unsworth3Simon J. O'Carroll4Elyce du Mez5Catherine E. Angel6E. Scott Graham7Department of Pharmacology, University of Auckland, Auckland 1023, New ZealandDepartment of Pharmacology, University of Auckland, Auckland 1023, New ZealandDepartment of Pharmacology, University of Auckland, Auckland 1023, New ZealandDepartment of Engineering Science, University of Auckland, Auckland 1010, New ZealandCentre for Brain Research, University of Auckland, Auckland 1023, New ZealandSchool of Biological Sciences, University of Auckland, Auckland 1010, New ZealandSchool of Biological Sciences, University of Auckland, Auckland 1010, New ZealandDepartment of Pharmacology, University of Auckland, Auckland 1023, New ZealandThe xCELLigence technology is a real-time cellular biosensor, which measures the net adhesion of cells to high-density gold electrode arrays printed on custom-designed E-plates. The strength of cellular adhesion is influenced by a myriad of factors that include cell type, cell viability, growth, migration, spreading and proliferation. We therefore hypothesised that xCELLigence biosensor technology would provide a valuable platform for the measurement of drug responses in a multitude of different experimental, clinical or pharmacological contexts. In this manuscript, we demonstrate how xCELLigence technology has been invaluable in the identification of (1) not only if cells respond to a particular drug, but (2) the window of drug responsiveness. The latter aspect is often left to educated guess work in classical end-point assays, whereas biosensor technology reveals the temporal profile of the response in real time, which enables both acute responses and longer term responses to be profiled within the same assay. In our experience, the xCELLigence biosensor technology is suitable for highly targeted drug assessment and also low to medium throughput drug screening, which produces high content temporal data in real time.http://www.mdpi.com/2079-6374/5/2/199xCELLigenceadhesioncytotoxicitycell deathdrugsresponsivenessCell Index |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dan Kho Christa MacDonald Rebecca Johnson Charles P. Unsworth Simon J. O'Carroll Elyce du Mez Catherine E. Angel E. Scott Graham |
spellingShingle |
Dan Kho Christa MacDonald Rebecca Johnson Charles P. Unsworth Simon J. O'Carroll Elyce du Mez Catherine E. Angel E. Scott Graham Application of xCELLigence RTCA Biosensor Technology for Revealing the Profile and Window of Drug Responsiveness in Real Time Biosensors xCELLigence adhesion cytotoxicity cell death drugs responsiveness Cell Index |
author_facet |
Dan Kho Christa MacDonald Rebecca Johnson Charles P. Unsworth Simon J. O'Carroll Elyce du Mez Catherine E. Angel E. Scott Graham |
author_sort |
Dan Kho |
title |
Application of xCELLigence RTCA Biosensor Technology for Revealing the Profile and Window of Drug Responsiveness in Real Time |
title_short |
Application of xCELLigence RTCA Biosensor Technology for Revealing the Profile and Window of Drug Responsiveness in Real Time |
title_full |
Application of xCELLigence RTCA Biosensor Technology for Revealing the Profile and Window of Drug Responsiveness in Real Time |
title_fullStr |
Application of xCELLigence RTCA Biosensor Technology for Revealing the Profile and Window of Drug Responsiveness in Real Time |
title_full_unstemmed |
Application of xCELLigence RTCA Biosensor Technology for Revealing the Profile and Window of Drug Responsiveness in Real Time |
title_sort |
application of xcelligence rtca biosensor technology for revealing the profile and window of drug responsiveness in real time |
publisher |
MDPI AG |
series |
Biosensors |
issn |
2079-6374 |
publishDate |
2015-04-01 |
description |
The xCELLigence technology is a real-time cellular biosensor, which measures the net adhesion of cells to high-density gold electrode arrays printed on custom-designed E-plates. The strength of cellular adhesion is influenced by a myriad of factors that include cell type, cell viability, growth, migration, spreading and proliferation. We therefore hypothesised that xCELLigence biosensor technology would provide a valuable platform for the measurement of drug responses in a multitude of different experimental, clinical or pharmacological contexts. In this manuscript, we demonstrate how xCELLigence technology has been invaluable in the identification of (1) not only if cells respond to a particular drug, but (2) the window of drug responsiveness. The latter aspect is often left to educated guess work in classical end-point assays, whereas biosensor technology reveals the temporal profile of the response in real time, which enables both acute responses and longer term responses to be profiled within the same assay. In our experience, the xCELLigence biosensor technology is suitable for highly targeted drug assessment and also low to medium throughput drug screening, which produces high content temporal data in real time. |
topic |
xCELLigence adhesion cytotoxicity cell death drugs responsiveness Cell Index |
url |
http://www.mdpi.com/2079-6374/5/2/199 |
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