Rapid free thiol rebound is a physiological response following cold‐induced vasoconstriction in healthy humans, primary Raynaud and systemic sclerosis
Abstract Raynaud's phenomenon (RP) is often the first sign of systemic sclerosis (SSc). Molecular mechanisms involved are incompletely understood, but reactive oxygen, nitrogen, and sulfur species are thought to play an important role in the pathogenesis of SSc. Free thiol groups play a protect...
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doaj-b826253d0bca4f4fa02b5b491b07225e2020-11-25T03:18:48ZengWileyPhysiological Reports2051-817X2019-03-0176n/an/a10.14814/phy2.14017Rapid free thiol rebound is a physiological response following cold‐induced vasoconstriction in healthy humans, primary Raynaud and systemic sclerosisAmaal Eman Abdulle0Anniek M. van Roon1Andries J. Smit2Andreas Pasch3Matijs vanMeurs4Hendrika Bootsma5Stephan J. L. Bakker6Mohammad Y. Said7Bernadette O. Fernandez8Martin Feelisch9Harry vanGoor10Douwe J. Mulder11Department of Internal Medicine Division Vascular Medicine University of Groningen – University Medical Centre Groningen Groningen The NetherlandsDepartment of Internal Medicine Division Vascular Medicine University of Groningen – University Medical Centre Groningen Groningen The NetherlandsDepartment of Internal Medicine Division Vascular Medicine University of Groningen – University Medical Centre Groningen Groningen The NetherlandsDepartment of Biomedical Research University of Bern Bern SwitzerlandDepartment of Critical Care University of Groningen – University Medical Centre Groningen Groningen The NetherlandsDepartment of Rheumatology and Clinical Immunology University of Groningen – University Medical Centre Groningen Groningen The NetherlandsDepartment of Internal Medicine Division of Nephrology University of Groningen – University Medical Centre Groningen Groningen The NetherlandsDepartment of Internal Medicine Division of Nephrology University of Groningen – University Medical Centre Groningen Groningen The NetherlandsClinical and Experimental Sciences Faculty of Medicine University of Southampton Southampton United KingdomClinical and Experimental Sciences Faculty of Medicine University of Southampton Southampton United KingdomDepartment of Pathology and Medical Biology Section Pathology University of Groningen – University Medical Centre Groningen Groningen The NetherlandsDepartment of Internal Medicine Division Vascular Medicine University of Groningen – University Medical Centre Groningen Groningen The NetherlandsAbstract Raynaud's phenomenon (RP) is often the first sign of systemic sclerosis (SSc). Molecular mechanisms involved are incompletely understood, but reactive oxygen, nitrogen, and sulfur species are thought to play an important role in the pathogenesis of SSc. Free thiol groups play a protective role against oxidative stress and may represent an attractive therapeutic target. We aimed to investigate the effects of hypothermia‐induced vasoconstriction on the responsiveness of redox‐related markers. Thirty participants (n = 10/group [SSc, primary Raynaud's phenomenon (PRP), healthy controls (HC)]) were included in this study. Fingertip photoelectric plethysmography was performed during a standardized cooling and recovery experiment. Venous blood was collected at four predetermined time points. Free thiols, NO‐derived species (nitros(yl)ated species, nitrite, nitrate), sulfate and endothelin‐1 were measured. Lower baseline concentrations of free thiols were observed in PRP and SSc patients (HC: 5.87 [5.41–5.99] μmol/g; PRP: 5.17 [4.74–5.61]; SSc 5.28 [4.75–5.80], P = 0.04). Redox‐related markers remained unchanged during cooling. However, an unexpected increase in systemic free thiol concentrations was observed in all groups during the recovery phase. The response of this marker differed between groups, with a higher increase found in SSc patients (HC Δ = 1.30 [1.48–1.17]; PRP Δ = 1.04 [1.06–1.03]; SSc Δ = 1.72 [1.13–1.49], P = 0.04). NO‐derived species, sulfate and endothelin‐1 levels remained unchanged throughout the recovery phase. This exploratory study sheds light on the rapid responsiveness of systemic free thiol concentrations following reperfusion, which may reflect overall redox balance. The robust response to reperfusion in SSc patients suggests that reductive systems involved in this response are functionally intact in these patients.https://doi.org/10.14814/phy2.14017Free thiolsnitric oxideRaynaud's phenomenonredox systemsystemic sclerosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Amaal Eman Abdulle Anniek M. van Roon Andries J. Smit Andreas Pasch Matijs vanMeurs Hendrika Bootsma Stephan J. L. Bakker Mohammad Y. Said Bernadette O. Fernandez Martin Feelisch Harry vanGoor Douwe J. Mulder |
spellingShingle |
Amaal Eman Abdulle Anniek M. van Roon Andries J. Smit Andreas Pasch Matijs vanMeurs Hendrika Bootsma Stephan J. L. Bakker Mohammad Y. Said Bernadette O. Fernandez Martin Feelisch Harry vanGoor Douwe J. Mulder Rapid free thiol rebound is a physiological response following cold‐induced vasoconstriction in healthy humans, primary Raynaud and systemic sclerosis Physiological Reports Free thiols nitric oxide Raynaud's phenomenon redox system systemic sclerosis |
author_facet |
Amaal Eman Abdulle Anniek M. van Roon Andries J. Smit Andreas Pasch Matijs vanMeurs Hendrika Bootsma Stephan J. L. Bakker Mohammad Y. Said Bernadette O. Fernandez Martin Feelisch Harry vanGoor Douwe J. Mulder |
author_sort |
Amaal Eman Abdulle |
title |
Rapid free thiol rebound is a physiological response following cold‐induced vasoconstriction in healthy humans, primary Raynaud and systemic sclerosis |
title_short |
Rapid free thiol rebound is a physiological response following cold‐induced vasoconstriction in healthy humans, primary Raynaud and systemic sclerosis |
title_full |
Rapid free thiol rebound is a physiological response following cold‐induced vasoconstriction in healthy humans, primary Raynaud and systemic sclerosis |
title_fullStr |
Rapid free thiol rebound is a physiological response following cold‐induced vasoconstriction in healthy humans, primary Raynaud and systemic sclerosis |
title_full_unstemmed |
Rapid free thiol rebound is a physiological response following cold‐induced vasoconstriction in healthy humans, primary Raynaud and systemic sclerosis |
title_sort |
rapid free thiol rebound is a physiological response following cold‐induced vasoconstriction in healthy humans, primary raynaud and systemic sclerosis |
publisher |
Wiley |
series |
Physiological Reports |
issn |
2051-817X |
publishDate |
2019-03-01 |
description |
Abstract Raynaud's phenomenon (RP) is often the first sign of systemic sclerosis (SSc). Molecular mechanisms involved are incompletely understood, but reactive oxygen, nitrogen, and sulfur species are thought to play an important role in the pathogenesis of SSc. Free thiol groups play a protective role against oxidative stress and may represent an attractive therapeutic target. We aimed to investigate the effects of hypothermia‐induced vasoconstriction on the responsiveness of redox‐related markers. Thirty participants (n = 10/group [SSc, primary Raynaud's phenomenon (PRP), healthy controls (HC)]) were included in this study. Fingertip photoelectric plethysmography was performed during a standardized cooling and recovery experiment. Venous blood was collected at four predetermined time points. Free thiols, NO‐derived species (nitros(yl)ated species, nitrite, nitrate), sulfate and endothelin‐1 were measured. Lower baseline concentrations of free thiols were observed in PRP and SSc patients (HC: 5.87 [5.41–5.99] μmol/g; PRP: 5.17 [4.74–5.61]; SSc 5.28 [4.75–5.80], P = 0.04). Redox‐related markers remained unchanged during cooling. However, an unexpected increase in systemic free thiol concentrations was observed in all groups during the recovery phase. The response of this marker differed between groups, with a higher increase found in SSc patients (HC Δ = 1.30 [1.48–1.17]; PRP Δ = 1.04 [1.06–1.03]; SSc Δ = 1.72 [1.13–1.49], P = 0.04). NO‐derived species, sulfate and endothelin‐1 levels remained unchanged throughout the recovery phase. This exploratory study sheds light on the rapid responsiveness of systemic free thiol concentrations following reperfusion, which may reflect overall redox balance. The robust response to reperfusion in SSc patients suggests that reductive systems involved in this response are functionally intact in these patients. |
topic |
Free thiols nitric oxide Raynaud's phenomenon redox system systemic sclerosis |
url |
https://doi.org/10.14814/phy2.14017 |
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