Differential gene expression in response to Fusarium oxysporum infection in resistant and susceptible genotypes of flax (Linum usitatissimum L.)
Abstract Background Flax (Linum usitatissimum L.) is a crop plant used for fiber and oil production. Although potentially high-yielding flax varieties have been developed, environmental stresses markedly decrease flax production. Among biotic stresses, Fusarium oxysporum f. sp. lini is recognized as...
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doaj-b82d5bcb341f44788bcc59f9650cdd412020-11-24T22:17:12ZengBMCBMC Plant Biology1471-22292017-12-0117S2294010.1186/s12870-017-1192-2Differential gene expression in response to Fusarium oxysporum infection in resistant and susceptible genotypes of flax (Linum usitatissimum L.)Alexey A. Dmitriev0George S. Krasnov1Tatiana A. Rozhmina2Roman O. Novakovskiy3Anastasiya V. Snezhkina4Maria S. Fedorova5Olga Yu. Yurkevich6Olga V. Muravenko7Nadezhda L. Bolsheva8Anna V. Kudryavtseva9Nataliya V. Melnikova10Engelhardt Institute of Molecular Biology, Russian Academy of SciencesEngelhardt Institute of Molecular Biology, Russian Academy of SciencesEngelhardt Institute of Molecular Biology, Russian Academy of SciencesEngelhardt Institute of Molecular Biology, Russian Academy of SciencesEngelhardt Institute of Molecular Biology, Russian Academy of SciencesEngelhardt Institute of Molecular Biology, Russian Academy of SciencesEngelhardt Institute of Molecular Biology, Russian Academy of SciencesEngelhardt Institute of Molecular Biology, Russian Academy of SciencesEngelhardt Institute of Molecular Biology, Russian Academy of SciencesEngelhardt Institute of Molecular Biology, Russian Academy of SciencesEngelhardt Institute of Molecular Biology, Russian Academy of SciencesAbstract Background Flax (Linum usitatissimum L.) is a crop plant used for fiber and oil production. Although potentially high-yielding flax varieties have been developed, environmental stresses markedly decrease flax production. Among biotic stresses, Fusarium oxysporum f. sp. lini is recognized as one of the most devastating flax pathogens. It causes wilt disease that is one of the major limiting factors for flax production worldwide. Breeding and cultivation of flax varieties resistant to F. oxysporum is the most effective method for controlling wilt disease. Although the mechanisms of flax response to Fusarium have been actively studied, data on the plant response to infection and resistance gene candidates are currently very limited. Results The transcriptomes of two resistant and two susceptible flax cultivars with respect to Fusarium wilt, as well as two resistant BC2F5 populations, which were grown under control conditions or inoculated with F. oxysporum, were sequenced using the Illumina platform. Genes showing changes in expression under F. oxysporum infection were identified in both resistant and susceptible flax genotypes. We observed the predominant overexpression of numerous genes that are involved in defense response. This was more pronounced in resistant cultivars. In susceptible cultivars, significant downregulation of genes involved in cell wall organization or biogenesis was observed in response to F. oxysporum. In the resistant genotypes, upregulation of genes related to NAD(P)H oxidase activity was detected. Upregulation of a number of genes, including that encoding beta-1,3-glucanase, was significantly greater in the cultivars and BC2F5 populations resistant to Fusarium wilt than in susceptible cultivars in response to F. oxysporum infection. Conclusions Using high-throughput sequencing, we identified genes involved in the early defense response of L. usitatissimum against the fungus F. oxysporum. In response to F. oxysporum infection, we detected changes in the expression of pathogenesis-related protein-encoding genes and genes involved in ROS production or related to cell wall biogenesis. Furthermore, we identified genes that were upregulated specifically in flax genotypes resistant to Fusarium wilt. We suggest that the identified genes in resistant cultivars and BC2F5 populations showing induced expression in response to F. oxysporum infection are the most promising resistance gene candidates.http://link.springer.com/article/10.1186/s12870-017-1192-2Linum usitatissimumFlaxBiotic stressFusarium oxysporumHigh-throughput sequencingROS |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alexey A. Dmitriev George S. Krasnov Tatiana A. Rozhmina Roman O. Novakovskiy Anastasiya V. Snezhkina Maria S. Fedorova Olga Yu. Yurkevich Olga V. Muravenko Nadezhda L. Bolsheva Anna V. Kudryavtseva Nataliya V. Melnikova |
spellingShingle |
Alexey A. Dmitriev George S. Krasnov Tatiana A. Rozhmina Roman O. Novakovskiy Anastasiya V. Snezhkina Maria S. Fedorova Olga Yu. Yurkevich Olga V. Muravenko Nadezhda L. Bolsheva Anna V. Kudryavtseva Nataliya V. Melnikova Differential gene expression in response to Fusarium oxysporum infection in resistant and susceptible genotypes of flax (Linum usitatissimum L.) BMC Plant Biology Linum usitatissimum Flax Biotic stress Fusarium oxysporum High-throughput sequencing ROS |
author_facet |
Alexey A. Dmitriev George S. Krasnov Tatiana A. Rozhmina Roman O. Novakovskiy Anastasiya V. Snezhkina Maria S. Fedorova Olga Yu. Yurkevich Olga V. Muravenko Nadezhda L. Bolsheva Anna V. Kudryavtseva Nataliya V. Melnikova |
author_sort |
Alexey A. Dmitriev |
title |
Differential gene expression in response to Fusarium oxysporum infection in resistant and susceptible genotypes of flax (Linum usitatissimum L.) |
title_short |
Differential gene expression in response to Fusarium oxysporum infection in resistant and susceptible genotypes of flax (Linum usitatissimum L.) |
title_full |
Differential gene expression in response to Fusarium oxysporum infection in resistant and susceptible genotypes of flax (Linum usitatissimum L.) |
title_fullStr |
Differential gene expression in response to Fusarium oxysporum infection in resistant and susceptible genotypes of flax (Linum usitatissimum L.) |
title_full_unstemmed |
Differential gene expression in response to Fusarium oxysporum infection in resistant and susceptible genotypes of flax (Linum usitatissimum L.) |
title_sort |
differential gene expression in response to fusarium oxysporum infection in resistant and susceptible genotypes of flax (linum usitatissimum l.) |
publisher |
BMC |
series |
BMC Plant Biology |
issn |
1471-2229 |
publishDate |
2017-12-01 |
description |
Abstract Background Flax (Linum usitatissimum L.) is a crop plant used for fiber and oil production. Although potentially high-yielding flax varieties have been developed, environmental stresses markedly decrease flax production. Among biotic stresses, Fusarium oxysporum f. sp. lini is recognized as one of the most devastating flax pathogens. It causes wilt disease that is one of the major limiting factors for flax production worldwide. Breeding and cultivation of flax varieties resistant to F. oxysporum is the most effective method for controlling wilt disease. Although the mechanisms of flax response to Fusarium have been actively studied, data on the plant response to infection and resistance gene candidates are currently very limited. Results The transcriptomes of two resistant and two susceptible flax cultivars with respect to Fusarium wilt, as well as two resistant BC2F5 populations, which were grown under control conditions or inoculated with F. oxysporum, were sequenced using the Illumina platform. Genes showing changes in expression under F. oxysporum infection were identified in both resistant and susceptible flax genotypes. We observed the predominant overexpression of numerous genes that are involved in defense response. This was more pronounced in resistant cultivars. In susceptible cultivars, significant downregulation of genes involved in cell wall organization or biogenesis was observed in response to F. oxysporum. In the resistant genotypes, upregulation of genes related to NAD(P)H oxidase activity was detected. Upregulation of a number of genes, including that encoding beta-1,3-glucanase, was significantly greater in the cultivars and BC2F5 populations resistant to Fusarium wilt than in susceptible cultivars in response to F. oxysporum infection. Conclusions Using high-throughput sequencing, we identified genes involved in the early defense response of L. usitatissimum against the fungus F. oxysporum. In response to F. oxysporum infection, we detected changes in the expression of pathogenesis-related protein-encoding genes and genes involved in ROS production or related to cell wall biogenesis. Furthermore, we identified genes that were upregulated specifically in flax genotypes resistant to Fusarium wilt. We suggest that the identified genes in resistant cultivars and BC2F5 populations showing induced expression in response to F. oxysporum infection are the most promising resistance gene candidates. |
topic |
Linum usitatissimum Flax Biotic stress Fusarium oxysporum High-throughput sequencing ROS |
url |
http://link.springer.com/article/10.1186/s12870-017-1192-2 |
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