First Draft Genome Assembly of the Malaysian Stingless Bee, <i>Heterotrigona itama</i> (Apidae, Meliponinae)
The Malaysian stingless bee industry is hugely dependent on wild colonies. Nevertheless, the availability of new queens to establish new colonies is insufficient to meet the growing demand for hives in the industry. <i>Heterotrigona itama</i> is primarily utilized for honey production in...
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doaj-b84a5f94937748349fd01f894abed3332020-12-01T00:00:13ZengMDPI AGData2306-57292020-11-01511211210.3390/data5040112First Draft Genome Assembly of the Malaysian Stingless Bee, <i>Heterotrigona itama</i> (Apidae, Meliponinae)Chien-Yeong Wee0Amin-Asyraf Tamizi1Nazrul-Hisham Nazaruddin2Siuk-Mun Ng3Jia-Shiun Khoo4Rosliza Jajuli5Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), Serdang 43400, Selangor, MalaysiaBiotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), Serdang 43400, Selangor, MalaysiaBiotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), Serdang 43400, Selangor, MalaysiaCodon Genomics S/B, Jalan Dutamas 7, Taman Dutamas Balakong, Seri Kembangan 43200, Selangor, MalaysiaCodon Genomics S/B, Jalan Dutamas 7, Taman Dutamas Balakong, Seri Kembangan 43200, Selangor, MalaysiaAgrobiodiversity and Environmental Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), Serdang 43400, Selangor, MalaysiaThe Malaysian stingless bee industry is hugely dependent on wild colonies. Nevertheless, the availability of new queens to establish new colonies is insufficient to meet the growing demand for hives in the industry. <i>Heterotrigona itama</i> is primarily utilized for honey production in the region and the major source of stingless bee colonies comes from the wild. To propagate new colonies domestically, a fundamental understanding of the biology of queen development, especially from the genomics aspect, is necessary. The whole genome was sequenced using a paired-end 150 strategy on the Illumina HiSeq X platform. The shotgun sequencing generated approximately 89 million raw pair-end reads with a total output of 13.37 Gb and a GC content of 37.31%. The genome size of the species was estimated to be approximately 272 Mb. Phylogenetic analysis showed <i>H. itama</i> are much more closely related to the bumble bee (<i>Bombus</i> spp.) than they are to the modern honey bee (<i>Apis</i> spp.). The genome data provided here are expected to contribute to a better understanding of the genetic aspect of queen differentiation as well as of important molecular pathways which are crucial for stingless bee biology, management and conservation.https://www.mdpi.com/2306-5729/5/4/112eusocial insectgenomicsIllumina sequencingpollinatorqueen differentiation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chien-Yeong Wee Amin-Asyraf Tamizi Nazrul-Hisham Nazaruddin Siuk-Mun Ng Jia-Shiun Khoo Rosliza Jajuli |
spellingShingle |
Chien-Yeong Wee Amin-Asyraf Tamizi Nazrul-Hisham Nazaruddin Siuk-Mun Ng Jia-Shiun Khoo Rosliza Jajuli First Draft Genome Assembly of the Malaysian Stingless Bee, <i>Heterotrigona itama</i> (Apidae, Meliponinae) Data eusocial insect genomics Illumina sequencing pollinator queen differentiation |
author_facet |
Chien-Yeong Wee Amin-Asyraf Tamizi Nazrul-Hisham Nazaruddin Siuk-Mun Ng Jia-Shiun Khoo Rosliza Jajuli |
author_sort |
Chien-Yeong Wee |
title |
First Draft Genome Assembly of the Malaysian Stingless Bee, <i>Heterotrigona itama</i> (Apidae, Meliponinae) |
title_short |
First Draft Genome Assembly of the Malaysian Stingless Bee, <i>Heterotrigona itama</i> (Apidae, Meliponinae) |
title_full |
First Draft Genome Assembly of the Malaysian Stingless Bee, <i>Heterotrigona itama</i> (Apidae, Meliponinae) |
title_fullStr |
First Draft Genome Assembly of the Malaysian Stingless Bee, <i>Heterotrigona itama</i> (Apidae, Meliponinae) |
title_full_unstemmed |
First Draft Genome Assembly of the Malaysian Stingless Bee, <i>Heterotrigona itama</i> (Apidae, Meliponinae) |
title_sort |
first draft genome assembly of the malaysian stingless bee, <i>heterotrigona itama</i> (apidae, meliponinae) |
publisher |
MDPI AG |
series |
Data |
issn |
2306-5729 |
publishDate |
2020-11-01 |
description |
The Malaysian stingless bee industry is hugely dependent on wild colonies. Nevertheless, the availability of new queens to establish new colonies is insufficient to meet the growing demand for hives in the industry. <i>Heterotrigona itama</i> is primarily utilized for honey production in the region and the major source of stingless bee colonies comes from the wild. To propagate new colonies domestically, a fundamental understanding of the biology of queen development, especially from the genomics aspect, is necessary. The whole genome was sequenced using a paired-end 150 strategy on the Illumina HiSeq X platform. The shotgun sequencing generated approximately 89 million raw pair-end reads with a total output of 13.37 Gb and a GC content of 37.31%. The genome size of the species was estimated to be approximately 272 Mb. Phylogenetic analysis showed <i>H. itama</i> are much more closely related to the bumble bee (<i>Bombus</i> spp.) than they are to the modern honey bee (<i>Apis</i> spp.). The genome data provided here are expected to contribute to a better understanding of the genetic aspect of queen differentiation as well as of important molecular pathways which are crucial for stingless bee biology, management and conservation. |
topic |
eusocial insect genomics Illumina sequencing pollinator queen differentiation |
url |
https://www.mdpi.com/2306-5729/5/4/112 |
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