Complete mitochondrial genome sequences of the northern spotted owl (Strix occidentalis caurina) and the barred owl (Strix varia; Aves: Strigiformes: Strigidae) confirm the presence of a duplicated control region
We report here the successful assembly of the complete mitochondrial genomes of the northern spotted owl (Strix occidentalis caurina) and the barred owl (S. varia). We utilized sequence data from two sequencing methodologies, Illumina paired-end sequence data with insert lengths ranging from approxi...
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doaj-b84b2f934bed434b861ffd799901a32f2020-11-24T22:33:45ZengPeerJ Inc.PeerJ2167-83592017-10-015e390110.7717/peerj.3901Complete mitochondrial genome sequences of the northern spotted owl (Strix occidentalis caurina) and the barred owl (Strix varia; Aves: Strigiformes: Strigidae) confirm the presence of a duplicated control regionZachary R. Hanna0James B. Henderson1Anna B. Sellas2Jérôme Fuchs3Rauri C.K. Bowie4John P. Dumbacher5Museum of Vertebrate Zoology, University of California, Berkeley, CA, United States of AmericaDepartment of Ornithology & Mammalogy, California Academy of Sciences, San Francisco, CA, United States of AmericaCenter for Comparative Genomics, California Academy of Sciences, San Francisco, CA, United States of AmericaDepartment of Ornithology & Mammalogy, California Academy of Sciences, San Francisco, CA, United States of AmericaMuseum of Vertebrate Zoology, University of California, Berkeley, CA, United States of AmericaDepartment of Ornithology & Mammalogy, California Academy of Sciences, San Francisco, CA, United States of AmericaWe report here the successful assembly of the complete mitochondrial genomes of the northern spotted owl (Strix occidentalis caurina) and the barred owl (S. varia). We utilized sequence data from two sequencing methodologies, Illumina paired-end sequence data with insert lengths ranging from approximately 250 nucleotides (nt) to 9,600 nt and read lengths from 100–375 nt and Sanger-derived sequences. We employed multiple assemblers and alignment methods to generate the final assemblies. The circular genomes of S. o. caurina and S. varia are comprised of 19,948 nt and 18,975 nt, respectively. Both code for two rRNAs, twenty-two tRNAs, and thirteen polypeptides. They both have duplicated control region sequences with complex repeat structures. We were not able to assemble the control regions solely using Illumina paired-end sequence data. By fully spanning the control regions, Sanger-derived sequences enabled accurate and complete assembly of these mitochondrial genomes. These are the first complete mitochondrial genome sequences of owls (Aves: Strigiformes) possessing duplicated control regions. We searched the nuclear genome of S. o. caurina for copies of mitochondrial genes and found at least nine separate stretches of nuclear copies of gene sequences originating in the mitochondrial genome (Numts). The Numts ranged from 226–19,522 nt in length and included copies of all mitochondrial genes except tRNAPro, ND6, and tRNAGlu. Strix occidentalis caurina and S. varia exhibited an average of 10.74% (8.68% uncorrected p-distance) divergence across the non-tRNA mitochondrial genes.https://peerj.com/articles/3901.pdfMitochondrial genomeBirdNorthern spotted owlBarred owlMtgenomemtDNA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zachary R. Hanna James B. Henderson Anna B. Sellas Jérôme Fuchs Rauri C.K. Bowie John P. Dumbacher |
spellingShingle |
Zachary R. Hanna James B. Henderson Anna B. Sellas Jérôme Fuchs Rauri C.K. Bowie John P. Dumbacher Complete mitochondrial genome sequences of the northern spotted owl (Strix occidentalis caurina) and the barred owl (Strix varia; Aves: Strigiformes: Strigidae) confirm the presence of a duplicated control region PeerJ Mitochondrial genome Bird Northern spotted owl Barred owl Mtgenome mtDNA |
author_facet |
Zachary R. Hanna James B. Henderson Anna B. Sellas Jérôme Fuchs Rauri C.K. Bowie John P. Dumbacher |
author_sort |
Zachary R. Hanna |
title |
Complete mitochondrial genome sequences of the northern spotted owl (Strix occidentalis caurina) and the barred owl (Strix varia; Aves: Strigiformes: Strigidae) confirm the presence of a duplicated control region |
title_short |
Complete mitochondrial genome sequences of the northern spotted owl (Strix occidentalis caurina) and the barred owl (Strix varia; Aves: Strigiformes: Strigidae) confirm the presence of a duplicated control region |
title_full |
Complete mitochondrial genome sequences of the northern spotted owl (Strix occidentalis caurina) and the barred owl (Strix varia; Aves: Strigiformes: Strigidae) confirm the presence of a duplicated control region |
title_fullStr |
Complete mitochondrial genome sequences of the northern spotted owl (Strix occidentalis caurina) and the barred owl (Strix varia; Aves: Strigiformes: Strigidae) confirm the presence of a duplicated control region |
title_full_unstemmed |
Complete mitochondrial genome sequences of the northern spotted owl (Strix occidentalis caurina) and the barred owl (Strix varia; Aves: Strigiformes: Strigidae) confirm the presence of a duplicated control region |
title_sort |
complete mitochondrial genome sequences of the northern spotted owl (strix occidentalis caurina) and the barred owl (strix varia; aves: strigiformes: strigidae) confirm the presence of a duplicated control region |
publisher |
PeerJ Inc. |
series |
PeerJ |
issn |
2167-8359 |
publishDate |
2017-10-01 |
description |
We report here the successful assembly of the complete mitochondrial genomes of the northern spotted owl (Strix occidentalis caurina) and the barred owl (S. varia). We utilized sequence data from two sequencing methodologies, Illumina paired-end sequence data with insert lengths ranging from approximately 250 nucleotides (nt) to 9,600 nt and read lengths from 100–375 nt and Sanger-derived sequences. We employed multiple assemblers and alignment methods to generate the final assemblies. The circular genomes of S. o. caurina and S. varia are comprised of 19,948 nt and 18,975 nt, respectively. Both code for two rRNAs, twenty-two tRNAs, and thirteen polypeptides. They both have duplicated control region sequences with complex repeat structures. We were not able to assemble the control regions solely using Illumina paired-end sequence data. By fully spanning the control regions, Sanger-derived sequences enabled accurate and complete assembly of these mitochondrial genomes. These are the first complete mitochondrial genome sequences of owls (Aves: Strigiformes) possessing duplicated control regions. We searched the nuclear genome of S. o. caurina for copies of mitochondrial genes and found at least nine separate stretches of nuclear copies of gene sequences originating in the mitochondrial genome (Numts). The Numts ranged from 226–19,522 nt in length and included copies of all mitochondrial genes except tRNAPro, ND6, and tRNAGlu. Strix occidentalis caurina and S. varia exhibited an average of 10.74% (8.68% uncorrected p-distance) divergence across the non-tRNA mitochondrial genes. |
topic |
Mitochondrial genome Bird Northern spotted owl Barred owl Mtgenome mtDNA |
url |
https://peerj.com/articles/3901.pdf |
work_keys_str_mv |
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