Testing human sperm chemotaxis: how to detect biased motion in population assays.

Testing human sperm chemotaxis: how to detect biased motion in population assays.

Biased motion of motile cells in a concentration gradient of a chemoattractant is frequently studied on the population level. This approach has been particularly employed in human sperm chemotactic assays, where the fraction of responsive cells is low and detection of biased motion depends on subtle...

Full description

Bibliographic Details
Main Authors: Leah Armon, S Roy Caplan, Michael Eisenbach, Benjamin M Friedrich
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3297605?pdf=render
id doaj-b85cec7c757d47c5ab0decafa7f722fc
record_format Article
spelling doaj-b85cec7c757d47c5ab0decafa7f722fc2020-11-25T02:36:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0173e3290910.1371/journal.pone.0032909Testing human sperm chemotaxis: how to detect biased motion in population assays.Leah ArmonS Roy CaplanMichael EisenbachBenjamin M FriedrichBiased motion of motile cells in a concentration gradient of a chemoattractant is frequently studied on the population level. This approach has been particularly employed in human sperm chemotactic assays, where the fraction of responsive cells is low and detection of biased motion depends on subtle differences. In these assays, statistical measures such as population odds ratios of swimming directions can be employed to infer chemotactic performance. Here, we report on an improved method to assess statistical significance of experimentally determined odds ratios and discuss the strong impact of data correlations that arise from the directional persistence of sperm swimming.http://europepmc.org/articles/PMC3297605?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Leah Armon
S Roy Caplan
Michael Eisenbach
Benjamin M Friedrich
spellingShingle Leah Armon
S Roy Caplan
Michael Eisenbach
Benjamin M Friedrich
Testing human sperm chemotaxis: how to detect biased motion in population assays.
PLoS ONE
author_facet Leah Armon
S Roy Caplan
Michael Eisenbach
Benjamin M Friedrich
author_sort Leah Armon
title Testing human sperm chemotaxis: how to detect biased motion in population assays.
title_short Testing human sperm chemotaxis: how to detect biased motion in population assays.
title_full Testing human sperm chemotaxis: how to detect biased motion in population assays.
title_fullStr Testing human sperm chemotaxis: how to detect biased motion in population assays.
title_full_unstemmed Testing human sperm chemotaxis: how to detect biased motion in population assays.
title_sort testing human sperm chemotaxis: how to detect biased motion in population assays.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2012-01-01
description Biased motion of motile cells in a concentration gradient of a chemoattractant is frequently studied on the population level. This approach has been particularly employed in human sperm chemotactic assays, where the fraction of responsive cells is low and detection of biased motion depends on subtle differences. In these assays, statistical measures such as population odds ratios of swimming directions can be employed to infer chemotactic performance. Here, we report on an improved method to assess statistical significance of experimentally determined odds ratios and discuss the strong impact of data correlations that arise from the directional persistence of sperm swimming.
url http://europepmc.org/articles/PMC3297605?pdf=render
work_keys_str_mv AT leaharmon testinghumanspermchemotaxishowtodetectbiasedmotioninpopulationassays
AT sroycaplan testinghumanspermchemotaxishowtodetectbiasedmotioninpopulationassays
AT michaeleisenbach testinghumanspermchemotaxishowtodetectbiasedmotioninpopulationassays
AT benjaminmfriedrich testinghumanspermchemotaxishowtodetectbiasedmotioninpopulationassays
_version_ 1724800252190392320

Similar Items