Metabolism of a lipid nanoemulsion resembling low-density lipoprotein in patients with grade III obesity
INTRODUCTION: Obesity increases triglyceride levels and decreases high-density lipoprotein concentrations in plasma. Artificial emulsions resembling lipidic plasma lipoprotein structures have been used to evaluate low-density lipoprotein metabolism. In grade III obesity, low density lipoprotein meta...
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2010-01-01
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doaj-b863b1e2cb2943a9a7d6c9af812a2e722020-11-24T23:39:26ZengFaculdade de Medicina / USPClinics1807-59321980-53222010-01-01651232710.1590/S1807-59322010000100005Metabolism of a lipid nanoemulsion resembling low-density lipoprotein in patients with grade III obesitySimone Alves DantasElisabeth Salvatori FickerCarmen G. C. VinagreBarbara Maria IanniRaul Cavalcante MaranhãoCharles MadyINTRODUCTION: Obesity increases triglyceride levels and decreases high-density lipoprotein concentrations in plasma. Artificial emulsions resembling lipidic plasma lipoprotein structures have been used to evaluate low-density lipoprotein metabolism. In grade III obesity, low density lipoprotein metabolism is poorly understood. OBJECTIVE: To evaluate the kinetics with which a cholesterol-rich emulsion (called a low-density emulsion) binds to low-density lipoprotein receptors in a group of patients with grade III obesity by the fractional clearance rate. METHODS: A low-density emulsion was labeled with [14C]-cholesterol ester and [³H]-triglycerides and injected intravenously into ten normolipidemic non-diabetic patients with grade III obesity [body mass index higher than 40 kg/m²] and into ten non-obese healthy controls. Blood samples were collected over 24 hours to determine the plasma decay curve and to calculate the fractional clearance rate. RESULTS: There was no difference regarding plasma levels of total cholesterol or low-density lipoprotein cholesterol between the two groups. The fractional clearance rate of triglycerides was 0.086 ± 0.044 in the obese group and 0.122 ± 0.026 in the controls (p = 0.040), and the fractional clearance rate of cholesterol ester (h-1) was 0.052 ± 0.021 in the obese subjects and 0.058 ± 0.015 (p = 0.971) in the controls. CONCLUSION: Grade III obese subjects exhibited normal low-density lipoprotein removal from plasma as tested by the nanoemulsion method, but triglyceride removal was slower.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322010000100005LDL metabolismgrade III obesityartificial emulsions with lipidic structureradioisotopestriglyceride |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Simone Alves Dantas Elisabeth Salvatori Ficker Carmen G. C. Vinagre Barbara Maria Ianni Raul Cavalcante Maranhão Charles Mady |
spellingShingle |
Simone Alves Dantas Elisabeth Salvatori Ficker Carmen G. C. Vinagre Barbara Maria Ianni Raul Cavalcante Maranhão Charles Mady Metabolism of a lipid nanoemulsion resembling low-density lipoprotein in patients with grade III obesity Clinics LDL metabolism grade III obesity artificial emulsions with lipidic structure radioisotopes triglyceride |
author_facet |
Simone Alves Dantas Elisabeth Salvatori Ficker Carmen G. C. Vinagre Barbara Maria Ianni Raul Cavalcante Maranhão Charles Mady |
author_sort |
Simone Alves Dantas |
title |
Metabolism of a lipid nanoemulsion resembling low-density lipoprotein in patients with grade III obesity |
title_short |
Metabolism of a lipid nanoemulsion resembling low-density lipoprotein in patients with grade III obesity |
title_full |
Metabolism of a lipid nanoemulsion resembling low-density lipoprotein in patients with grade III obesity |
title_fullStr |
Metabolism of a lipid nanoemulsion resembling low-density lipoprotein in patients with grade III obesity |
title_full_unstemmed |
Metabolism of a lipid nanoemulsion resembling low-density lipoprotein in patients with grade III obesity |
title_sort |
metabolism of a lipid nanoemulsion resembling low-density lipoprotein in patients with grade iii obesity |
publisher |
Faculdade de Medicina / USP |
series |
Clinics |
issn |
1807-5932 1980-5322 |
publishDate |
2010-01-01 |
description |
INTRODUCTION: Obesity increases triglyceride levels and decreases high-density lipoprotein concentrations in plasma. Artificial emulsions resembling lipidic plasma lipoprotein structures have been used to evaluate low-density lipoprotein metabolism. In grade III obesity, low density lipoprotein metabolism is poorly understood. OBJECTIVE: To evaluate the kinetics with which a cholesterol-rich emulsion (called a low-density emulsion) binds to low-density lipoprotein receptors in a group of patients with grade III obesity by the fractional clearance rate. METHODS: A low-density emulsion was labeled with [14C]-cholesterol ester and [³H]-triglycerides and injected intravenously into ten normolipidemic non-diabetic patients with grade III obesity [body mass index higher than 40 kg/m²] and into ten non-obese healthy controls. Blood samples were collected over 24 hours to determine the plasma decay curve and to calculate the fractional clearance rate. RESULTS: There was no difference regarding plasma levels of total cholesterol or low-density lipoprotein cholesterol between the two groups. The fractional clearance rate of triglycerides was 0.086 ± 0.044 in the obese group and 0.122 ± 0.026 in the controls (p = 0.040), and the fractional clearance rate of cholesterol ester (h-1) was 0.052 ± 0.021 in the obese subjects and 0.058 ± 0.015 (p = 0.971) in the controls. CONCLUSION: Grade III obese subjects exhibited normal low-density lipoprotein removal from plasma as tested by the nanoemulsion method, but triglyceride removal was slower. |
topic |
LDL metabolism grade III obesity artificial emulsions with lipidic structure radioisotopes triglyceride |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322010000100005 |
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