The contribution of genes required for anaerobic respiration to the virulence of Salmonella enterica serovar Gallinarum for chickens
Salmonella enterica serovar Gallinarum (SG) is an intracellular pathogen of chickens. To survive, to invade and to multiply in the intestinal tract and intracellularly it depends on its ability to produce energy in anaerobic conditions. The fumarate reductase (frdABCD), dimethyl sulfoxide (DMSO)-tri...
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Sociedade Brasileira de Microbiologia
2009-12-01
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doaj-b868241edcbf47539a765392fac7082e2020-11-25T00:56:25ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1517-83821678-44052009-12-01404994100110.1590/S1517-83822009000400035The contribution of genes required for anaerobic respiration to the virulence of Salmonella enterica serovar Gallinarum for chickensJ.B. PaivaR.A.C. Penha FilhoE.A. PereiraM.V.F. LemosP.A. BarrowM.A. LovellA. Berchieri JrSalmonella enterica serovar Gallinarum (SG) is an intracellular pathogen of chickens. To survive, to invade and to multiply in the intestinal tract and intracellularly it depends on its ability to produce energy in anaerobic conditions. The fumarate reductase (frdABCD), dimethyl sulfoxide (DMSO)-trimethylamine N-oxide (TMAO) reductase (dmsABC), and nitrate reductase (narGHIJ) operons in Salmonella Typhimurium (STM) encode enzymes involved in anaerobic respiration to the electron acceptors fumarate, DMSO, TMAO, and nitrate, respectively. They are regulated in response to nitrate and oxygen availability and changes in cell growth rate. In this study mortality rates of chickens challenged with mutants of Salmonella Gallinarum, which were defective in utilising anaerobic electron acceptors, were assessed in comparison to group of bird challenged with wild strain. The greatest degree of attenuation was observed with mutations affecting nitrate reductase (napA, narG) with additional attenuations induced by a mutation affecting fumarate reductase (frdA) and a double mutant (dmsA torC) affecting DMSO and TMAO reductase.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400035Salmonella Gallinarumanaerobic genespoultrymutations |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
J.B. Paiva R.A.C. Penha Filho E.A. Pereira M.V.F. Lemos P.A. Barrow M.A. Lovell A. Berchieri Jr |
spellingShingle |
J.B. Paiva R.A.C. Penha Filho E.A. Pereira M.V.F. Lemos P.A. Barrow M.A. Lovell A. Berchieri Jr The contribution of genes required for anaerobic respiration to the virulence of Salmonella enterica serovar Gallinarum for chickens Brazilian Journal of Microbiology Salmonella Gallinarum anaerobic genes poultry mutations |
author_facet |
J.B. Paiva R.A.C. Penha Filho E.A. Pereira M.V.F. Lemos P.A. Barrow M.A. Lovell A. Berchieri Jr |
author_sort |
J.B. Paiva |
title |
The contribution of genes required for anaerobic respiration to the virulence of Salmonella enterica serovar Gallinarum for chickens |
title_short |
The contribution of genes required for anaerobic respiration to the virulence of Salmonella enterica serovar Gallinarum for chickens |
title_full |
The contribution of genes required for anaerobic respiration to the virulence of Salmonella enterica serovar Gallinarum for chickens |
title_fullStr |
The contribution of genes required for anaerobic respiration to the virulence of Salmonella enterica serovar Gallinarum for chickens |
title_full_unstemmed |
The contribution of genes required for anaerobic respiration to the virulence of Salmonella enterica serovar Gallinarum for chickens |
title_sort |
contribution of genes required for anaerobic respiration to the virulence of salmonella enterica serovar gallinarum for chickens |
publisher |
Sociedade Brasileira de Microbiologia |
series |
Brazilian Journal of Microbiology |
issn |
1517-8382 1678-4405 |
publishDate |
2009-12-01 |
description |
Salmonella enterica serovar Gallinarum (SG) is an intracellular pathogen of chickens. To survive, to invade and to multiply in the intestinal tract and intracellularly it depends on its ability to produce energy in anaerobic conditions. The fumarate reductase (frdABCD), dimethyl sulfoxide (DMSO)-trimethylamine N-oxide (TMAO) reductase (dmsABC), and nitrate reductase (narGHIJ) operons in Salmonella Typhimurium (STM) encode enzymes involved in anaerobic respiration to the electron acceptors fumarate, DMSO, TMAO, and nitrate, respectively. They are regulated in response to nitrate and oxygen availability and changes in cell growth rate. In this study mortality rates of chickens challenged with mutants of Salmonella Gallinarum, which were defective in utilising anaerobic electron acceptors, were assessed in comparison to group of bird challenged with wild strain. The greatest degree of attenuation was observed with mutations affecting nitrate reductase (napA, narG) with additional attenuations induced by a mutation affecting fumarate reductase (frdA) and a double mutant (dmsA torC) affecting DMSO and TMAO reductase. |
topic |
Salmonella Gallinarum anaerobic genes poultry mutations |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400035 |
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