Fraud identification in beef sausage in Tehran province using mitochondrial genes of animal species
The aim of this study was to develop a species-specific PCR method for rapid identification of meat species in order to detect any potential fraud in meat products. For cattle and poultry, a set of primers were designed based on the mitochondrial cytochrome b and subsequently a 183 bp as well as 274...
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Islamic Azad University, Tabriz Branch
2014-05-01
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doaj-b87276d630cd4181aa70c10616f67c132020-11-25T01:02:33ZfasIslamic Azad University, Tabriz BranchBihdāsht-i Mavādd-i Ghaz̠āyī2228-76472476-69682014-05-0141 (13) بهار8189517093Fraud identification in beef sausage in Tehran province using mitochondrial genes of animal speciesH.S , Hosseni0F Tafvizi1M Tajabadi Ebrahimi2A Sharifan3- Assistant Professor of Food Science and Technology, College of Agriculture & Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran.Assistant Professor, Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran- Assistant Professor, Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, IranAssistant Professor of Food Science and Technology, College of Food Science and Industrial, Science and Research Branch, Islamic Azad University, Tehran, IranThe aim of this study was to develop a species-specific PCR method for rapid identification of meat species in order to detect any potential fraud in meat products. For cattle and poultry, a set of primers were designed based on the mitochondrial cytochrome b and subsequently a 183 bp as well as 274 bp fragments were amplified for poultry and beef samples, respectively. For the study, ten different brands of beef sausage were obtained across Tehran province. Results of PCR assay demonstrated that unlike their labels, all of the samples were contained poultry residuals. Moreover, beef offal was detected only in five samples. Since no cross-reaction was observed among the different samples, PCR could be considered as a sensitive and specific method for the identification of meat varieties. In addition, high-speed operation and easiness of the technique are the other advantages of PCR method. Therefore, this technique has the capability for sensitive and specific detection of meat adulteration in sausages.http://jfh.iaut.ac.ir/article_517093_4cc6f7f4023d15d08abac2d2080214fa.pdfsausagesFraud identificationMeat species identificationPCR |
collection |
DOAJ |
language |
fas |
format |
Article |
sources |
DOAJ |
author |
H.S , Hosseni F Tafvizi M Tajabadi Ebrahimi A Sharifan |
spellingShingle |
H.S , Hosseni F Tafvizi M Tajabadi Ebrahimi A Sharifan Fraud identification in beef sausage in Tehran province using mitochondrial genes of animal species Bihdāsht-i Mavādd-i Ghaz̠āyī sausages Fraud identification Meat species identification PCR |
author_facet |
H.S , Hosseni F Tafvizi M Tajabadi Ebrahimi A Sharifan |
author_sort |
H.S , Hosseni |
title |
Fraud identification in beef sausage in Tehran province using mitochondrial genes of animal species |
title_short |
Fraud identification in beef sausage in Tehran province using mitochondrial genes of animal species |
title_full |
Fraud identification in beef sausage in Tehran province using mitochondrial genes of animal species |
title_fullStr |
Fraud identification in beef sausage in Tehran province using mitochondrial genes of animal species |
title_full_unstemmed |
Fraud identification in beef sausage in Tehran province using mitochondrial genes of animal species |
title_sort |
fraud identification in beef sausage in tehran province using mitochondrial genes of animal species |
publisher |
Islamic Azad University, Tabriz Branch |
series |
Bihdāsht-i Mavādd-i Ghaz̠āyī |
issn |
2228-7647 2476-6968 |
publishDate |
2014-05-01 |
description |
The aim of this study was to develop a species-specific PCR method for rapid identification of meat species in order to detect any potential fraud in meat products. For cattle and poultry, a set of primers were designed based on the mitochondrial cytochrome b and subsequently a 183 bp as well as 274 bp fragments were amplified for poultry and beef samples, respectively. For the study, ten different brands of beef sausage were obtained across Tehran province. Results of PCR assay demonstrated that unlike their labels, all of the samples were contained poultry residuals. Moreover, beef offal was detected only in five samples. Since no cross-reaction was observed among the different samples, PCR could be considered as a sensitive and specific method for the identification of meat varieties. In addition, high-speed operation and easiness of the technique are the other advantages of PCR method. Therefore, this technique has the capability for sensitive and specific detection of meat adulteration in sausages. |
topic |
sausages Fraud identification Meat species identification PCR |
url |
http://jfh.iaut.ac.ir/article_517093_4cc6f7f4023d15d08abac2d2080214fa.pdf |
work_keys_str_mv |
AT hshosseni fraudidentificationinbeefsausageintehranprovinceusingmitochondrialgenesofanimalspecies AT ftafvizi fraudidentificationinbeefsausageintehranprovinceusingmitochondrialgenesofanimalspecies AT mtajabadiebrahimi fraudidentificationinbeefsausageintehranprovinceusingmitochondrialgenesofanimalspecies AT asharifan fraudidentificationinbeefsausageintehranprovinceusingmitochondrialgenesofanimalspecies |
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1725204147346604032 |