Improved inflammatory balance of human skeletal muscle during exercise after supplementations of the ginseng-based steroid Rg1.
The purpose of the study was to determine the effect of ginseng-based steroid Rg1 on TNF-alpha and IL-10 gene expression in human skeletal muscle against exercise challenge, as well as on its ergogenic outcomes. Randomized double-blind placebo-controlled crossover trials were performed, separated by...
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doaj-b885a0582a0f4fd19b34a119b812ad092020-11-24T21:24:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01101e011638710.1371/journal.pone.0116387Improved inflammatory balance of human skeletal muscle during exercise after supplementations of the ginseng-based steroid Rg1.Chien-Wen HouShin-Da LeeChung-Lan KaoI-Shiung ChengYu-Nan LinSheng-Ju ChuangChung-Yu ChenJohn L IvyChih-Yang HuangChia-Hua KuoThe purpose of the study was to determine the effect of ginseng-based steroid Rg1 on TNF-alpha and IL-10 gene expression in human skeletal muscle against exercise challenge, as well as on its ergogenic outcomes. Randomized double-blind placebo-controlled crossover trials were performed, separated by a 4-week washout. Healthy young men were randomized into two groups and received capsule containing either 5 mg of Rg1 or Placebo one night and one hour before exercise. Muscle biopsies were conducted at baseline, immediately and 3 h after a standardized 60-min cycle ergometer exercise. While treatment differences in glycogen depletion rate of biopsied quadriceps muscle during exercise did not reach statistical significance, Rg1 supplementations enhanced post-exercise glycogen replenishment and increased citrate synthase activity in the skeletal muscle 3 h after exercise, concurrent with improved meal tolerance during recovery (P<0.05). Rg1 suppressed the exercise-induced increases in thiobarbituric acids reactive substance (TBARS) and reversed the increased TNF-alpha and decreased IL-10 mRNA of quadriceps muscle against the exercise challenge. PGC-1 alpha and GLUT4 mRNAs of exercised muscle were not affected by Rg1. Maximal aerobic capacity (VO2max) was not changed by Rg1. However, cycling time to exhaustion at 80% VO2max increased significantly by ~20% (P<0.05).Our result suggests that Rg1 is an ergogenic component of ginseng, which can minimize unwanted lipid peroxidation of exercised human skeletal muscle, and attenuate pro-inflammatory shift under exercise challenge.http://europepmc.org/articles/PMC4305310?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chien-Wen Hou Shin-Da Lee Chung-Lan Kao I-Shiung Cheng Yu-Nan Lin Sheng-Ju Chuang Chung-Yu Chen John L Ivy Chih-Yang Huang Chia-Hua Kuo |
spellingShingle |
Chien-Wen Hou Shin-Da Lee Chung-Lan Kao I-Shiung Cheng Yu-Nan Lin Sheng-Ju Chuang Chung-Yu Chen John L Ivy Chih-Yang Huang Chia-Hua Kuo Improved inflammatory balance of human skeletal muscle during exercise after supplementations of the ginseng-based steroid Rg1. PLoS ONE |
author_facet |
Chien-Wen Hou Shin-Da Lee Chung-Lan Kao I-Shiung Cheng Yu-Nan Lin Sheng-Ju Chuang Chung-Yu Chen John L Ivy Chih-Yang Huang Chia-Hua Kuo |
author_sort |
Chien-Wen Hou |
title |
Improved inflammatory balance of human skeletal muscle during exercise after supplementations of the ginseng-based steroid Rg1. |
title_short |
Improved inflammatory balance of human skeletal muscle during exercise after supplementations of the ginseng-based steroid Rg1. |
title_full |
Improved inflammatory balance of human skeletal muscle during exercise after supplementations of the ginseng-based steroid Rg1. |
title_fullStr |
Improved inflammatory balance of human skeletal muscle during exercise after supplementations of the ginseng-based steroid Rg1. |
title_full_unstemmed |
Improved inflammatory balance of human skeletal muscle during exercise after supplementations of the ginseng-based steroid Rg1. |
title_sort |
improved inflammatory balance of human skeletal muscle during exercise after supplementations of the ginseng-based steroid rg1. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
The purpose of the study was to determine the effect of ginseng-based steroid Rg1 on TNF-alpha and IL-10 gene expression in human skeletal muscle against exercise challenge, as well as on its ergogenic outcomes. Randomized double-blind placebo-controlled crossover trials were performed, separated by a 4-week washout. Healthy young men were randomized into two groups and received capsule containing either 5 mg of Rg1 or Placebo one night and one hour before exercise. Muscle biopsies were conducted at baseline, immediately and 3 h after a standardized 60-min cycle ergometer exercise. While treatment differences in glycogen depletion rate of biopsied quadriceps muscle during exercise did not reach statistical significance, Rg1 supplementations enhanced post-exercise glycogen replenishment and increased citrate synthase activity in the skeletal muscle 3 h after exercise, concurrent with improved meal tolerance during recovery (P<0.05). Rg1 suppressed the exercise-induced increases in thiobarbituric acids reactive substance (TBARS) and reversed the increased TNF-alpha and decreased IL-10 mRNA of quadriceps muscle against the exercise challenge. PGC-1 alpha and GLUT4 mRNAs of exercised muscle were not affected by Rg1. Maximal aerobic capacity (VO2max) was not changed by Rg1. However, cycling time to exhaustion at 80% VO2max increased significantly by ~20% (P<0.05).Our result suggests that Rg1 is an ergogenic component of ginseng, which can minimize unwanted lipid peroxidation of exercised human skeletal muscle, and attenuate pro-inflammatory shift under exercise challenge. |
url |
http://europepmc.org/articles/PMC4305310?pdf=render |
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