Rapid Construction of Stable Infectious Full-Length cDNA Clone of Papaya Leaf Distortion Mosaic Virus Using In-Fusion Cloning

Rapid Construction of Stable Infectious Full-Length cDNA Clone of Papaya Leaf Distortion Mosaic Virus Using In-Fusion Cloning

Papaya leaf distortion mosaic virus (PLDMV) is becoming a threat to papaya and transgenic papaya resistant to the related pathogen, papaya ringspot virus (PRSV). The generation of infectious viral clones is an essential step for reverse-genetics studies of viral gene function and cross-protection. I...

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Bibliographic Details
Main Authors: Decai Tuo, Wentao Shen, Pu Yan, Xiaoying Li, Peng Zhou
Format: Article
Language:English
Published: MDPI AG 2015-12-01
Series:Viruses
Subjects:
PLDMV
infectious cDNA clone
In-Fusion
intron
papaya
Online Access:http://www.mdpi.com/1999-4915/7/12/2935

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