Interleukin-13, Interleukin-10, Interferon-gamma and IDO Production in Response to Home Dust Mites in Allergic Asthma
BACKGROUND: Allergic asthma is a degenerative atopic disease caused by allergic or hypersensitivity type-1. More than 50% of people with allergic asthma are caused by the presence of house dust mites (HDMs) allergens. METHODS: The cellular immunity response was evaluated through a peripheral blood...
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doaj-b8c4ae5fdc1549159053510212aabf7c2021-03-01T03:20:55ZengSecretariat of The Indonesian Biomedical JournalIndonesian Biomedical Journal2085-32972355-91792019-08-01112194910.18585/inabj.v11i2.714315Interleukin-13, Interleukin-10, Interferon-gamma and IDO Production in Response to Home Dust Mites in Allergic AsthmaCityta Putri Kwarta0Heri Wibowo1Yordan Khaedir2Iris Rengganis3Hanny Siti Nuraeni4Magister Program in Biomedical Sciences, Universitas Indonesia, Jl. Salemba Raya No.5, JakartaDepartment of Parasitology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.5, JakartaFaculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.5, JakartaDivision of Allergy Immunology, Department of Internal Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo Central General Hospital, Jl. Diponogoro No.71, JakartaMagister Program in Biomedical Sciences, Universitas Indonesia, Jl. Salemba Raya No.5, JakartaBACKGROUND: Allergic asthma is a degenerative atopic disease caused by allergic or hypersensitivity type-1. More than 50% of people with allergic asthma are caused by the presence of house dust mites (HDMs) allergens. METHODS: The cellular immunity response was evaluated through a peripheral blood mononuclear cell (PBMC) culture isolated from blood, using the ficoll gradient technique. Subjects were atopic asthma groups and non-atopic asthma groups. PBMC from each subject cultured was stimulated with HDMs allergen, then incubated in a CO2 5% incubator, 37o C for 72 hours. With the multiplex assay method, interferon (IFN)-γ, interleukin (IL)-13 and IL-10 were measured, meanwhile indoleamine 2,3-dioxygenase level (IDO) was measured by the enzyme-linked immunosorbent assay (ELISA) sandwich methods. RESULTS: The IFN-γ production in the supernatant of PBMC cultures was stimulated by phytohemagglutinin (PHA), Roswell Park Memorial Institute (RPMI) medium and allergens. The IFN-γ production in allergen-stimulated supernatants showed higher level of IFN-γ in the nonatopic group (4,681,455±3,434,851) than atopic group (4,363,300±2,067,941) even though it was not statistically significant (p=0.078). There were no differences between the mean of IL-13 production in atopic asthma group and non-atopic group. The IL-10 production in allergenstimulated supernatants was shown to be higher in nonatopic group and were statistically significantly different (p=0.015). The IDO production in allergen-stimulated supernatants was shown to be higher in the non-atopic group (272,231±269,564) than in the actopic group (13,273±400), and it was significantly different (p=0.007). CONCLUSION: Cellular immune profile of subjects with allergic asthma to Dermatophagoides pterronyssinus (Der p) is characterized by a type-2 inflammatory response that is dominant compared to type-1 inflammation (higher IL-13 ratio compared to IFN-γ) and to the role of anti-inflammation (higher IL-13 ratio compared to IL-10). The decline in IDO production in allergic asthma subjects to Der p is thought to be related to the low cellular immune response in expressing IFN-γ compared to IL-13. KEYWORDS: interleukin-13, interleukin-10, IDO, PBMC, asthmahttps://inabj.org/index.php/ibj/article/view/714 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cityta Putri Kwarta Heri Wibowo Yordan Khaedir Iris Rengganis Hanny Siti Nuraeni |
spellingShingle |
Cityta Putri Kwarta Heri Wibowo Yordan Khaedir Iris Rengganis Hanny Siti Nuraeni Interleukin-13, Interleukin-10, Interferon-gamma and IDO Production in Response to Home Dust Mites in Allergic Asthma Indonesian Biomedical Journal |
author_facet |
Cityta Putri Kwarta Heri Wibowo Yordan Khaedir Iris Rengganis Hanny Siti Nuraeni |
author_sort |
Cityta Putri Kwarta |
title |
Interleukin-13, Interleukin-10, Interferon-gamma and IDO Production in Response to Home Dust Mites in Allergic Asthma |
title_short |
Interleukin-13, Interleukin-10, Interferon-gamma and IDO Production in Response to Home Dust Mites in Allergic Asthma |
title_full |
Interleukin-13, Interleukin-10, Interferon-gamma and IDO Production in Response to Home Dust Mites in Allergic Asthma |
title_fullStr |
Interleukin-13, Interleukin-10, Interferon-gamma and IDO Production in Response to Home Dust Mites in Allergic Asthma |
title_full_unstemmed |
Interleukin-13, Interleukin-10, Interferon-gamma and IDO Production in Response to Home Dust Mites in Allergic Asthma |
title_sort |
interleukin-13, interleukin-10, interferon-gamma and ido production in response to home dust mites in allergic asthma |
publisher |
Secretariat of The Indonesian Biomedical Journal |
series |
Indonesian Biomedical Journal |
issn |
2085-3297 2355-9179 |
publishDate |
2019-08-01 |
description |
BACKGROUND: Allergic asthma is a degenerative atopic disease caused by allergic or hypersensitivity type-1. More than 50% of people with allergic asthma are caused by the presence of house dust mites (HDMs) allergens.
METHODS: The cellular immunity response was evaluated through a peripheral blood mononuclear cell (PBMC) culture isolated from blood, using the ficoll gradient technique. Subjects were atopic asthma groups and non-atopic asthma groups. PBMC from each subject cultured was stimulated with HDMs allergen, then incubated in a CO2 5% incubator, 37o C for 72 hours. With the multiplex assay method, interferon (IFN)-γ, interleukin (IL)-13 and IL-10 were measured, meanwhile indoleamine 2,3-dioxygenase level (IDO) was measured by the enzyme-linked immunosorbent assay (ELISA) sandwich methods.
RESULTS: The IFN-γ production in the supernatant of PBMC cultures was stimulated by phytohemagglutinin (PHA), Roswell Park Memorial Institute (RPMI) medium and allergens. The IFN-γ production in allergen-stimulated supernatants showed higher level of IFN-γ in the nonatopic group (4,681,455±3,434,851) than atopic group (4,363,300±2,067,941) even though it was not statistically significant (p=0.078). There were no differences between the mean of IL-13 production in atopic asthma group and non-atopic group. The IL-10 production in allergenstimulated supernatants was shown to be higher in nonatopic group and were statistically significantly different (p=0.015). The IDO production in allergen-stimulated supernatants was shown to be higher in the non-atopic group (272,231±269,564) than in the actopic group (13,273±400), and it was significantly different (p=0.007).
CONCLUSION: Cellular immune profile of subjects with allergic asthma to Dermatophagoides pterronyssinus (Der p) is characterized by a type-2 inflammatory response that is dominant compared to type-1 inflammation (higher IL-13 ratio compared to IFN-γ) and to the role of anti-inflammation (higher IL-13 ratio compared to IL-10). The decline in IDO production in allergic asthma subjects to Der p is thought to be related to the low cellular immune response in expressing IFN-γ compared to IL-13.
KEYWORDS: interleukin-13, interleukin-10, IDO, PBMC, asthma |
url |
https://inabj.org/index.php/ibj/article/view/714 |
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