Application of bacterial and yeast biosurfactants for enhanced removal and biodegradation of motor oil from contaminated sand

Background: This study investigated the potential application of two biosurfactants for enhanced removal capability and biodegradation of motor oil contaminated sand under laboratory conditions. The biosurfactants were produced by the yeast Candida sphaerica and by the bacterium Bacillus sp. cultiva...

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Main Authors: Marcos J. Chaprão, Isabela N.S. Ferreira, Priscilla F. Correa, Raquel D. Rufino, Juliana M. Luna, Elias J. Silva, Leonie A. Sarubbo
Format: Article
Language:English
Published: Elsevier 2015-11-01
Series:Electronic Journal of Biotechnology
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0717345815001219
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spelling doaj-b8c7b9ff329a467da78723af9f87076d2020-11-25T01:02:25ZengElsevierElectronic Journal of Biotechnology0717-34582015-11-0118647147910.1016/j.ejbt.2015.09.005Application of bacterial and yeast biosurfactants for enhanced removal and biodegradation of motor oil from contaminated sandMarcos J. Chaprão0Isabela N.S. Ferreira1Priscilla F. Correa2Raquel D. Rufino3Juliana M. Luna4Elias J. Silva5Leonie A. Sarubbo6Centro de Ciências e Tecnologia, Universidade Católica de Pernambuco, Rua do Príncipe 526, Boa Vista, Recife, Pernambuco, BrazilCentro de Ciências e Tecnologia, Universidade Católica de Pernambuco, Rua do Príncipe 526, Boa Vista, Recife, Pernambuco, BrazilCentro de Ciências e Tecnologia, Universidade Católica de Pernambuco, Rua do Príncipe 526, Boa Vista, Recife, Pernambuco, BrazilCentro de Ciências e Tecnologia, Universidade Católica de Pernambuco, Rua do Príncipe 526, Boa Vista, Recife, Pernambuco, BrazilCentro de Ciências e Tecnologia, Universidade Católica de Pernambuco, Rua do Príncipe 526, Boa Vista, Recife, Pernambuco, BrazilCentro de Gestão de Tecnologia e Inovação (CGTI), Rua da Praia 11, São José, Recife, Pernambuco, BrazilCentro de Ciências e Tecnologia, Universidade Católica de Pernambuco, Rua do Príncipe 526, Boa Vista, Recife, Pernambuco, BrazilBackground: This study investigated the potential application of two biosurfactants for enhanced removal capability and biodegradation of motor oil contaminated sand under laboratory conditions. The biosurfactants were produced by the yeast Candida sphaerica and by the bacterium Bacillus sp. cultivated in low-cost substrates. The ability of removing motor oil from soil by the two biosurfactants was identified and compared with that of the synthetic surfactants Tween 80 and Triton X-100. Results: Both crude and isolated biosurfactants showed excellent effectiveness on motor oil removal from contaminated sand under kinetic conditions (70–90%), while the synthetic surfactants removed between 55 and 80% of the oil. A contact time of 5–10 min under agitation seemed to be enough for oil removal with the biosurfactants and synthetic surfactants tested. The crude and the isolated biosurfactant from C. sphaerica were able to remove high percentages of motor oil from packed columns (around 90%) when compared to the biosurfactant from Bacillus sp. (40%). For the degradation experiments conducted in motor oil contaminated sand enriched with sugar cane molasses, however, oil degradation reached almost 100% after 90 d in the presence of Bacillus sp. cells, while the percentage of oil degradation did not exceed 50% in the presence of C. sphaerica. The presence of the biosurfactants increased the degradation rate in 10–20%, especially during the first 45 d, indicating that biosurfactants acted as efficient enhancers for hydrocarbon biodegradation. Conclusions: The results indicated the biosurfactants enhancing capability on both removal and rate of motor oil biodegradation in soil systems.http://www.sciencedirect.com/science/article/pii/S0717345815001219Bacillus sp.BioremediationCandida sphaericaPetroleumSand-packed column
collection DOAJ
language English
format Article
sources DOAJ
author Marcos J. Chaprão
Isabela N.S. Ferreira
Priscilla F. Correa
Raquel D. Rufino
Juliana M. Luna
Elias J. Silva
Leonie A. Sarubbo
spellingShingle Marcos J. Chaprão
Isabela N.S. Ferreira
Priscilla F. Correa
Raquel D. Rufino
Juliana M. Luna
Elias J. Silva
Leonie A. Sarubbo
Application of bacterial and yeast biosurfactants for enhanced removal and biodegradation of motor oil from contaminated sand
Electronic Journal of Biotechnology
Bacillus sp.
Bioremediation
Candida sphaerica
Petroleum
Sand-packed column
author_facet Marcos J. Chaprão
Isabela N.S. Ferreira
Priscilla F. Correa
Raquel D. Rufino
Juliana M. Luna
Elias J. Silva
Leonie A. Sarubbo
author_sort Marcos J. Chaprão
title Application of bacterial and yeast biosurfactants for enhanced removal and biodegradation of motor oil from contaminated sand
title_short Application of bacterial and yeast biosurfactants for enhanced removal and biodegradation of motor oil from contaminated sand
title_full Application of bacterial and yeast biosurfactants for enhanced removal and biodegradation of motor oil from contaminated sand
title_fullStr Application of bacterial and yeast biosurfactants for enhanced removal and biodegradation of motor oil from contaminated sand
title_full_unstemmed Application of bacterial and yeast biosurfactants for enhanced removal and biodegradation of motor oil from contaminated sand
title_sort application of bacterial and yeast biosurfactants for enhanced removal and biodegradation of motor oil from contaminated sand
publisher Elsevier
series Electronic Journal of Biotechnology
issn 0717-3458
publishDate 2015-11-01
description Background: This study investigated the potential application of two biosurfactants for enhanced removal capability and biodegradation of motor oil contaminated sand under laboratory conditions. The biosurfactants were produced by the yeast Candida sphaerica and by the bacterium Bacillus sp. cultivated in low-cost substrates. The ability of removing motor oil from soil by the two biosurfactants was identified and compared with that of the synthetic surfactants Tween 80 and Triton X-100. Results: Both crude and isolated biosurfactants showed excellent effectiveness on motor oil removal from contaminated sand under kinetic conditions (70–90%), while the synthetic surfactants removed between 55 and 80% of the oil. A contact time of 5–10 min under agitation seemed to be enough for oil removal with the biosurfactants and synthetic surfactants tested. The crude and the isolated biosurfactant from C. sphaerica were able to remove high percentages of motor oil from packed columns (around 90%) when compared to the biosurfactant from Bacillus sp. (40%). For the degradation experiments conducted in motor oil contaminated sand enriched with sugar cane molasses, however, oil degradation reached almost 100% after 90 d in the presence of Bacillus sp. cells, while the percentage of oil degradation did not exceed 50% in the presence of C. sphaerica. The presence of the biosurfactants increased the degradation rate in 10–20%, especially during the first 45 d, indicating that biosurfactants acted as efficient enhancers for hydrocarbon biodegradation. Conclusions: The results indicated the biosurfactants enhancing capability on both removal and rate of motor oil biodegradation in soil systems.
topic Bacillus sp.
Bioremediation
Candida sphaerica
Petroleum
Sand-packed column
url http://www.sciencedirect.com/science/article/pii/S0717345815001219
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