A five-year survey of dematiaceous fungi in a tropical hospital reveals potential opportunistic species.
Dematiaceous fungi (black fungi) are a heterogeneous group of fungi present in diverse environments worldwide. Many species in this group are known to cause allergic reactions and potentially fatal diseases in humans and animals, especially in tropical and subtropical climates. This study represents...
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doaj-b8ced63d0f554c6c92f09a1c1e60e8c22020-11-25T01:27:34ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0198e10435210.1371/journal.pone.0104352A five-year survey of dematiaceous fungi in a tropical hospital reveals potential opportunistic species.Su Mei YewChai Ling ChanKok Wei LeeShiang Ling NaRuixin TanChee-Choong HohWai-Yan YeeYun Fong NgeowKee Peng NgDematiaceous fungi (black fungi) are a heterogeneous group of fungi present in diverse environments worldwide. Many species in this group are known to cause allergic reactions and potentially fatal diseases in humans and animals, especially in tropical and subtropical climates. This study represents the first survey of dematiaceous fungi in Malaysia and provides observations on their diversity as well as in vitro response to antifungal drugs. Seventy-five strains isolated from various clinical specimens were identified by morphology as well as an internal transcribed spacer (ITS)-based phylogenetic analysis. The combined molecular and conventional approach enabled the identification of three classes of the Ascomycota phylum and 16 genera, the most common being Cladosporium, Cochliobolus and Neoscytalidium. Several of the species identified have not been associated before with human infections. Among 8 antifungal agents tested, the azoles posaconazole (96%), voriconazole (90.7%), ketoconazole (86.7%) and itraconazole (85.3%) showed in vitro activity (MIC ≤ 1 µg/mL) to the largest number of strains, followed by anidulafungin (89.3%), caspofungin (74.7%) and amphotericin B (70.7%). Fluconazole appeared to be the least effective with only 10.7% of isolates showing in vitro susceptibility. Overall, almost half (45.3%) of the isolates showed reduced susceptibility (MIC >1 µg/mL) to at least one antifungal agent, and three strains (one Pyrenochaeta unguis-hominis and two Nigrospora oryzae) showed potential multidrug resistance.http://europepmc.org/articles/PMC4123927?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Su Mei Yew Chai Ling Chan Kok Wei Lee Shiang Ling Na Ruixin Tan Chee-Choong Hoh Wai-Yan Yee Yun Fong Ngeow Kee Peng Ng |
spellingShingle |
Su Mei Yew Chai Ling Chan Kok Wei Lee Shiang Ling Na Ruixin Tan Chee-Choong Hoh Wai-Yan Yee Yun Fong Ngeow Kee Peng Ng A five-year survey of dematiaceous fungi in a tropical hospital reveals potential opportunistic species. PLoS ONE |
author_facet |
Su Mei Yew Chai Ling Chan Kok Wei Lee Shiang Ling Na Ruixin Tan Chee-Choong Hoh Wai-Yan Yee Yun Fong Ngeow Kee Peng Ng |
author_sort |
Su Mei Yew |
title |
A five-year survey of dematiaceous fungi in a tropical hospital reveals potential opportunistic species. |
title_short |
A five-year survey of dematiaceous fungi in a tropical hospital reveals potential opportunistic species. |
title_full |
A five-year survey of dematiaceous fungi in a tropical hospital reveals potential opportunistic species. |
title_fullStr |
A five-year survey of dematiaceous fungi in a tropical hospital reveals potential opportunistic species. |
title_full_unstemmed |
A five-year survey of dematiaceous fungi in a tropical hospital reveals potential opportunistic species. |
title_sort |
five-year survey of dematiaceous fungi in a tropical hospital reveals potential opportunistic species. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Dematiaceous fungi (black fungi) are a heterogeneous group of fungi present in diverse environments worldwide. Many species in this group are known to cause allergic reactions and potentially fatal diseases in humans and animals, especially in tropical and subtropical climates. This study represents the first survey of dematiaceous fungi in Malaysia and provides observations on their diversity as well as in vitro response to antifungal drugs. Seventy-five strains isolated from various clinical specimens were identified by morphology as well as an internal transcribed spacer (ITS)-based phylogenetic analysis. The combined molecular and conventional approach enabled the identification of three classes of the Ascomycota phylum and 16 genera, the most common being Cladosporium, Cochliobolus and Neoscytalidium. Several of the species identified have not been associated before with human infections. Among 8 antifungal agents tested, the azoles posaconazole (96%), voriconazole (90.7%), ketoconazole (86.7%) and itraconazole (85.3%) showed in vitro activity (MIC ≤ 1 µg/mL) to the largest number of strains, followed by anidulafungin (89.3%), caspofungin (74.7%) and amphotericin B (70.7%). Fluconazole appeared to be the least effective with only 10.7% of isolates showing in vitro susceptibility. Overall, almost half (45.3%) of the isolates showed reduced susceptibility (MIC >1 µg/mL) to at least one antifungal agent, and three strains (one Pyrenochaeta unguis-hominis and two Nigrospora oryzae) showed potential multidrug resistance. |
url |
http://europepmc.org/articles/PMC4123927?pdf=render |
work_keys_str_mv |
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