Performance of LDBio <i>Aspergillus</i> WB and ICT Antibody Detection in Chronic Pulmonary Aspergillosis

• Main Authors: Anna Rozaliyani, Findra Setianingrum, Sresta Azahra, Asriyani Abdullah, Ayu Eka Fatril, Harmi Rosianawati, Erlina Burhan, Diah Handayani, Arief Riadi Arifin, Jamal Zaini, Mulyati Tugiran, Robiatul Adawiyah, Ridhawati Syam, Heri Wibowo, Retno Wahyuningsih, Chris Kosmidis, David W Denning
• Format: Article
• Language: English
• Published: MDPI AG 2021-04-01
• Series: Journal of Fungi
• Subjects: chronic pulmonary aspergillosis; Western blot; immunochromatography
• Online Access: https://www.mdpi.com/2309-608X/7/4/311

The detection of <i>Aspergillus</i> antibody has a key role in the diagnosis of chronic pulmonary aspergillosis. Western blot (WB) and immunochromatography (ICT) lateral flow detection of <i>Aspergillus</i> antibody can be used as confirmatory and screening assays but their comparative performance in TB patients is not known. This study investigated the performance of these assays among 88 post-tuberculosis patients with suspected CPA. Sensitivity, specificity, receiver operating curve (ROC), area under-curve (AUC) and the agreement between two assays were evaluated. Both WB and ICT showed good sensitivity (80% and 85%, respectively) for detection of <i>Aspergillus</i> antibodies. Substantial agreement (0.716) between these assays was also obtained. The highest AUC result (0.804) was achieved with the combination of WB and ICT. The global intensity of WB correlated with the severity of symptoms in CPA group (<i>p</i> = 0.001). The combination of WB and ICT may increase specificity in CPA diagnosis.