Comparative Study of Gold and Carbon Nanoparticles in Nucleic Acid Lateral Flow Assay
A lateral flow assay (LFA) is a paper-based, point-of-need test designed to detect a specific analyte in complex samples in low-resource settings. Although LFA has been successfully used in different applications, its use is still limited when high sensitivity is required, especially in the diagnosi...
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doaj-b8e6d4c5673e411ab97063e5e93782ab2021-03-16T00:05:55ZengMDPI AGNanomaterials2079-49912021-03-011174174110.3390/nano11030741Comparative Study of Gold and Carbon Nanoparticles in Nucleic Acid Lateral Flow AssayJuan Carlos Porras0Mireia Bernuz1Jennifer Marfa2Arnau Pallares-Rusiñol3Mercè Martí4María Isabel Pividori5Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainGrup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainGrup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainGrup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainInstitute of Biotechnology and Biomedicine, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainGrup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainA lateral flow assay (LFA) is a paper-based, point-of-need test designed to detect a specific analyte in complex samples in low-resource settings. Although LFA has been successfully used in different applications, its use is still limited when high sensitivity is required, especially in the diagnosis of an early-stage condition. The limit of detection (LOD) is clearly related to the signal-generating system used to achieve the visual readout, in many cases involving nanoparticles coupled to a biomolecule, which, when combined, provides sensitivity and specificity, respectively. While colloidal gold is currently the most-used label, other detection systems are being developed. Carbon nanoparticles (CNPs) demonstrate outstanding features to improve the sensitivity of this technology by producing an increased contrast in the paper background. Based on the necessity of sensitivity improvement, the aim of this work is a comparative study, in terms of analytical performance, between commercial streptavidin gold nanoparticles (streptAv-AuNPs) and avidin carbon nanoparticles (Av-CNPs) in a nucleic acid lateral flow assay. The visual LOD of the method was calculated by serial dilution of the DNA template, ranging from 0.0 to 7 pg μL<sup>−1</sup>/1.5 × 10<sup>4</sup> CFU mL<sup>−1</sup>). The LFA achieved visual detection of as low as 2.2 × 10<sup>−2</sup> pg μL<sup>−1</sup> using Av-CNPs and 8.4 × 10<sup>−2</sup> pg μL<sup>−1</sup> using streptAv-AuNPs. These LODs could be obtained without the assistance of any instrumentation. The results demonstrate that CNPs showed an increased sensitivity, achieving the nanomolar range even by visual inspection. Furthermore, CNPs are the cheapest labels, and the suspensions are very stable and easy to modify.https://www.mdpi.com/2079-4991/11/3/741carbon blackgold nanoparticlesrapid diagnostic testslateral flow assay |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Juan Carlos Porras Mireia Bernuz Jennifer Marfa Arnau Pallares-Rusiñol Mercè Martí María Isabel Pividori |
spellingShingle |
Juan Carlos Porras Mireia Bernuz Jennifer Marfa Arnau Pallares-Rusiñol Mercè Martí María Isabel Pividori Comparative Study of Gold and Carbon Nanoparticles in Nucleic Acid Lateral Flow Assay Nanomaterials carbon black gold nanoparticles rapid diagnostic tests lateral flow assay |
author_facet |
Juan Carlos Porras Mireia Bernuz Jennifer Marfa Arnau Pallares-Rusiñol Mercè Martí María Isabel Pividori |
author_sort |
Juan Carlos Porras |
title |
Comparative Study of Gold and Carbon Nanoparticles in Nucleic Acid Lateral Flow Assay |
title_short |
Comparative Study of Gold and Carbon Nanoparticles in Nucleic Acid Lateral Flow Assay |
title_full |
Comparative Study of Gold and Carbon Nanoparticles in Nucleic Acid Lateral Flow Assay |
title_fullStr |
Comparative Study of Gold and Carbon Nanoparticles in Nucleic Acid Lateral Flow Assay |
title_full_unstemmed |
Comparative Study of Gold and Carbon Nanoparticles in Nucleic Acid Lateral Flow Assay |
title_sort |
comparative study of gold and carbon nanoparticles in nucleic acid lateral flow assay |
publisher |
MDPI AG |
series |
Nanomaterials |
issn |
2079-4991 |
publishDate |
2021-03-01 |
description |
A lateral flow assay (LFA) is a paper-based, point-of-need test designed to detect a specific analyte in complex samples in low-resource settings. Although LFA has been successfully used in different applications, its use is still limited when high sensitivity is required, especially in the diagnosis of an early-stage condition. The limit of detection (LOD) is clearly related to the signal-generating system used to achieve the visual readout, in many cases involving nanoparticles coupled to a biomolecule, which, when combined, provides sensitivity and specificity, respectively. While colloidal gold is currently the most-used label, other detection systems are being developed. Carbon nanoparticles (CNPs) demonstrate outstanding features to improve the sensitivity of this technology by producing an increased contrast in the paper background. Based on the necessity of sensitivity improvement, the aim of this work is a comparative study, in terms of analytical performance, between commercial streptavidin gold nanoparticles (streptAv-AuNPs) and avidin carbon nanoparticles (Av-CNPs) in a nucleic acid lateral flow assay. The visual LOD of the method was calculated by serial dilution of the DNA template, ranging from 0.0 to 7 pg μL<sup>−1</sup>/1.5 × 10<sup>4</sup> CFU mL<sup>−1</sup>). The LFA achieved visual detection of as low as 2.2 × 10<sup>−2</sup> pg μL<sup>−1</sup> using Av-CNPs and 8.4 × 10<sup>−2</sup> pg μL<sup>−1</sup> using streptAv-AuNPs. These LODs could be obtained without the assistance of any instrumentation. The results demonstrate that CNPs showed an increased sensitivity, achieving the nanomolar range even by visual inspection. Furthermore, CNPs are the cheapest labels, and the suspensions are very stable and easy to modify. |
topic |
carbon black gold nanoparticles rapid diagnostic tests lateral flow assay |
url |
https://www.mdpi.com/2079-4991/11/3/741 |
work_keys_str_mv |
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