Species diversity and insecticide resistance within the Anopheles hyrcanus group in Ubon Ratchathani Province, Thailand
Abstract Background Members of the Anopheles hyrcanus group have been incriminated as important malaria vectors. This study aims to identify the species and explore the insecticide susceptibility profile within the Anopheles hyrcanus group in Ubon Ratchathani Province, northeastern Thailand where in...
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doaj-b8f841a5b8fe471cb4815d28b588ffc92020-11-25T03:56:37ZengBMCParasites & Vectors1756-33052020-10-0113111310.1186/s13071-020-04389-4Species diversity and insecticide resistance within the Anopheles hyrcanus group in Ubon Ratchathani Province, ThailandAnchana Sumarnrote0Hans J. Overgaard1Vincent Corbel2Kanutcharee Thanispong3Theeraphap Chareonviriyaphap4Sylvie Manguin5Department of Entomology, Faculty of Agriculture at Kamphaeng Saen, Kasetsart UniversityDepartment of Entomology, Faculty of Agriculture at Kamphaeng Saen, Kasetsart UniversityDepartment of Entomology, Faculty of Agriculture at Kamphaeng Saen, Kasetsart UniversityBureau of Vector-borne Disease, Department of Disease control, Ministry of Public HealthDepartment of Entomology, Faculty of Agriculture, Kasetsart UniversityHydroSciences Montpellier (HSM), Institut de Recherche pour le Développement (IRD), CNRS, Université MontpellierAbstract Background Members of the Anopheles hyrcanus group have been incriminated as important malaria vectors. This study aims to identify the species and explore the insecticide susceptibility profile within the Anopheles hyrcanus group in Ubon Ratchathani Province, northeastern Thailand where increasing numbers of malaria cases were reported in 2014. Methods Between 2013 and 2015, five rounds of mosquito collections were conducted using human landing and cattle bait techniques during both the rainy and dry seasons. Anopheles mosquitoes were morphologically identified and their insecticide susceptibility status was investigated. Synergist bioassays were carried out with An. hyrcanus (s.l.) due to their resistance to all insecticides. An ITS2-PCR assay was conducted to identify to species the Hyrcanus group specimens. Results Out of 10,361 Anopheles females collected, representing 18 taxa in 2 subgenera, 71.8% were morphologically identified as belonging to the Hyrcanus Group (subgenus Anopheles), followed by An. barbirostris group (7.9%), An. nivipes (6.5%), An. philippinensis (5.9%) and the other 14 Anopheles species. Specimens of the Hyrcanus Group were more prevalent during the rainy season and were found to be highly zoophilic. Anopheles hyrcanus (s.l.) was active throughout the night, with an early peak of activity between 18:00 h and 21:00 h. ITS2-PCR assay conducted on 603 DNA samples from specimens within the Hyrcanus Group showed the presence of five sisters species. Anopheles peditaeniatus was the most abundant species (90.5%, n = 546), followed by An. nitidus (4.5%, n = 27), An. nigerrimus (4.3%, n = 26), An. argyropus (0.5%, n = 3), and An. sinensis (0.2%, n = 1). All An. hyrcanus (s.l.) specimens that were found resistant to insecticides (deltamethrin 0.05%, permethrin 0.75% and DDT 4% and synergist tests) belonged to An. peditaeniatus. The degree of resistance in An. peditaeniatus to each of these three insecticides was approximately 50%. Addition of PBO (Piperonyl butoxide), but not DEF (S.S.S-tributyl phosphotritioate), seemed to restore susceptibility, indicating a potential role of oxidases as a detoxifying enzyme resistance mechanism. Conclusions A better understanding of mosquito diversity related to host preference, biting activity and insecticide resistance status will facilitate the implementation of locally adapted vector control strategies.http://link.springer.com/article/10.1186/s13071-020-04389-4Anopheles hyrcanusMalaria vectorsSpecies diversityInsecticide resistanceUbon Ratchathani ProvinceThailand |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anchana Sumarnrote Hans J. Overgaard Vincent Corbel Kanutcharee Thanispong Theeraphap Chareonviriyaphap Sylvie Manguin |
spellingShingle |
Anchana Sumarnrote Hans J. Overgaard Vincent Corbel Kanutcharee Thanispong Theeraphap Chareonviriyaphap Sylvie Manguin Species diversity and insecticide resistance within the Anopheles hyrcanus group in Ubon Ratchathani Province, Thailand Parasites & Vectors Anopheles hyrcanus Malaria vectors Species diversity Insecticide resistance Ubon Ratchathani Province Thailand |
author_facet |
Anchana Sumarnrote Hans J. Overgaard Vincent Corbel Kanutcharee Thanispong Theeraphap Chareonviriyaphap Sylvie Manguin |
author_sort |
Anchana Sumarnrote |
title |
Species diversity and insecticide resistance within the Anopheles hyrcanus group in Ubon Ratchathani Province, Thailand |
title_short |
Species diversity and insecticide resistance within the Anopheles hyrcanus group in Ubon Ratchathani Province, Thailand |
title_full |
Species diversity and insecticide resistance within the Anopheles hyrcanus group in Ubon Ratchathani Province, Thailand |
title_fullStr |
Species diversity and insecticide resistance within the Anopheles hyrcanus group in Ubon Ratchathani Province, Thailand |
title_full_unstemmed |
Species diversity and insecticide resistance within the Anopheles hyrcanus group in Ubon Ratchathani Province, Thailand |
title_sort |
species diversity and insecticide resistance within the anopheles hyrcanus group in ubon ratchathani province, thailand |
publisher |
BMC |
series |
Parasites & Vectors |
issn |
1756-3305 |
publishDate |
2020-10-01 |
description |
Abstract Background Members of the Anopheles hyrcanus group have been incriminated as important malaria vectors. This study aims to identify the species and explore the insecticide susceptibility profile within the Anopheles hyrcanus group in Ubon Ratchathani Province, northeastern Thailand where increasing numbers of malaria cases were reported in 2014. Methods Between 2013 and 2015, five rounds of mosquito collections were conducted using human landing and cattle bait techniques during both the rainy and dry seasons. Anopheles mosquitoes were morphologically identified and their insecticide susceptibility status was investigated. Synergist bioassays were carried out with An. hyrcanus (s.l.) due to their resistance to all insecticides. An ITS2-PCR assay was conducted to identify to species the Hyrcanus group specimens. Results Out of 10,361 Anopheles females collected, representing 18 taxa in 2 subgenera, 71.8% were morphologically identified as belonging to the Hyrcanus Group (subgenus Anopheles), followed by An. barbirostris group (7.9%), An. nivipes (6.5%), An. philippinensis (5.9%) and the other 14 Anopheles species. Specimens of the Hyrcanus Group were more prevalent during the rainy season and were found to be highly zoophilic. Anopheles hyrcanus (s.l.) was active throughout the night, with an early peak of activity between 18:00 h and 21:00 h. ITS2-PCR assay conducted on 603 DNA samples from specimens within the Hyrcanus Group showed the presence of five sisters species. Anopheles peditaeniatus was the most abundant species (90.5%, n = 546), followed by An. nitidus (4.5%, n = 27), An. nigerrimus (4.3%, n = 26), An. argyropus (0.5%, n = 3), and An. sinensis (0.2%, n = 1). All An. hyrcanus (s.l.) specimens that were found resistant to insecticides (deltamethrin 0.05%, permethrin 0.75% and DDT 4% and synergist tests) belonged to An. peditaeniatus. The degree of resistance in An. peditaeniatus to each of these three insecticides was approximately 50%. Addition of PBO (Piperonyl butoxide), but not DEF (S.S.S-tributyl phosphotritioate), seemed to restore susceptibility, indicating a potential role of oxidases as a detoxifying enzyme resistance mechanism. Conclusions A better understanding of mosquito diversity related to host preference, biting activity and insecticide resistance status will facilitate the implementation of locally adapted vector control strategies. |
topic |
Anopheles hyrcanus Malaria vectors Species diversity Insecticide resistance Ubon Ratchathani Province Thailand |
url |
http://link.springer.com/article/10.1186/s13071-020-04389-4 |
work_keys_str_mv |
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