Exploring the Photodynamic Properties of Two Antiproliferative Benzodiazopyrrole Derivatives
The identification of molecules whose biological activity can be properly modulated by light is a promising therapeutic approach aimed to improve drug selectivity and efficacy on the molecular target and to limit the side effects compared to traditional drugs. Recently, two photo-switchable diastere...
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doaj-b8fe83a37cec459b9eb3eb086af582b62020-11-25T03:32:00ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-02-01214124610.3390/ijms21041246ijms21041246Exploring the Photodynamic Properties of Two Antiproliferative Benzodiazopyrrole DerivativesConcetta Imperatore0Mohammadhassan Valadan1Luciana Tartaglione2Marco Persico3Anna Ramunno4Marialuisa Menna5Marcello Casertano6Carmela Dell’Aversano7Manjot Singh8Maria Luisa d’Aulisio Garigliota9Francesco Bajardi10Elena Morelli11Caterina Fattorusso12Carlo Altucci13Michela Varra14Department of Pharmacy, University of Naples Federico II, 80131 Naples, ItalyDepartment of Physics “Ettore Pancini”, University of Naples Federico II, 80126 Naples, ItalyDepartment of Pharmacy, University of Naples Federico II, 80131 Naples, ItalyDepartment of Pharmacy, University of Naples Federico II, 80131 Naples, ItalyDepartment of Pharmacy/DIFARMA, University of Salerno, 84084 Fisciano, Salerno, ItalyDepartment of Pharmacy, University of Naples Federico II, 80131 Naples, ItalyDepartment of Pharmacy, University of Naples Federico II, 80131 Naples, ItalyDepartment of Pharmacy, University of Naples Federico II, 80131 Naples, ItalyDepartment of Physics “Ettore Pancini”, University of Naples Federico II, 80126 Naples, ItalyDepartment of Pharmacy/DIFARMA, University of Salerno, 84084 Fisciano, Salerno, ItalyDepartment of Physics “Ettore Pancini”, University of Naples Federico II, 80126 Naples, ItalyDepartment of Pharmacy, University of Naples Federico II, 80131 Naples, ItalyDepartment of Pharmacy, University of Naples Federico II, 80131 Naples, ItalyDepartment of Physics “Ettore Pancini”, University of Naples Federico II, 80126 Naples, ItalyDepartment of Pharmacy, University of Naples Federico II, 80131 Naples, ItalyThe identification of molecules whose biological activity can be properly modulated by light is a promising therapeutic approach aimed to improve drug selectivity and efficacy on the molecular target and to limit the side effects compared to traditional drugs. Recently, two photo-switchable diastereomeric benzodiazopyrrole derivatives <b>1RR</b> and <b>1RS</b> have been reported as microtubules targeting agents (MTAs) on human colorectal carcinoma p53 null cell line (HCT 116 p53-/-). Their IC<sub>50</sub> was enhanced upon Light Emitting Diode (LED) irradiation at 435 nm and was related to their <i>cis</i> form. Here we have investigated the photo-responsive behavior of the acid derivatives of <b>1RR</b> and <b>1RS</b>, namely, <i>d</i><b>1RR</b> and <i>d</i><b>1RS</b>, in phosphate buffer solutions at different pH. The comparison of the UV spectra, acquired before and after LED irradiation, indicated that the <i>trans</i><i>→</i><i>cis</i> conversion of <i>d</i><b>1RR</b> and <i>d</i><b>1RS</b> is affected by the degree of ionization. The apparent rate constants were calculated from the kinetic data by means of fast UV spectroscopy and the conformers of the putative ionic species present in solution (pH range: 5.7−8.0) were modelled. Taken together, our experimental and theoretical results suggest that the photo-conversions of <i>trans</i> <i>d</i><b>1RR</b>/<i>d</i><b>1RS</b> into the corresponding <i>cis</i> forms and the thermal decay of <i>cis</i> <i>d</i><b>1RR</b>/<i>d</i><b>1RS</b> are dependent on the presence of diazonium form of <i>d</i><b>1RR</b>/<i>d</i><b>1RS</b>. Finally, a photo-reaction was detected only for <i>d</i><b>1RR</b> after prolonged LED irradiation in acidic medium, and the resulting product was characterized by means of Liquid Chromatography coupled to High resolution Mass Spectrometry (LC-HRMS) and Nuclear Magnetic Resonance (NMR) spectroscopy.https://www.mdpi.com/1422-0067/21/4/1246photoswitchable azoheteroarenediazo derivative<i>cis</i>-<i>trans</i> conversionfast uv spectroscopylc-hrmsconformational analysisdft optimization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Concetta Imperatore Mohammadhassan Valadan Luciana Tartaglione Marco Persico Anna Ramunno Marialuisa Menna Marcello Casertano Carmela Dell’Aversano Manjot Singh Maria Luisa d’Aulisio Garigliota Francesco Bajardi Elena Morelli Caterina Fattorusso Carlo Altucci Michela Varra |
spellingShingle |
Concetta Imperatore Mohammadhassan Valadan Luciana Tartaglione Marco Persico Anna Ramunno Marialuisa Menna Marcello Casertano Carmela Dell’Aversano Manjot Singh Maria Luisa d’Aulisio Garigliota Francesco Bajardi Elena Morelli Caterina Fattorusso Carlo Altucci Michela Varra Exploring the Photodynamic Properties of Two Antiproliferative Benzodiazopyrrole Derivatives International Journal of Molecular Sciences photoswitchable azoheteroarene diazo derivative <i>cis</i>-<i>trans</i> conversion fast uv spectroscopy lc-hrms conformational analysis dft optimization |
author_facet |
Concetta Imperatore Mohammadhassan Valadan Luciana Tartaglione Marco Persico Anna Ramunno Marialuisa Menna Marcello Casertano Carmela Dell’Aversano Manjot Singh Maria Luisa d’Aulisio Garigliota Francesco Bajardi Elena Morelli Caterina Fattorusso Carlo Altucci Michela Varra |
author_sort |
Concetta Imperatore |
title |
Exploring the Photodynamic Properties of Two Antiproliferative Benzodiazopyrrole Derivatives |
title_short |
Exploring the Photodynamic Properties of Two Antiproliferative Benzodiazopyrrole Derivatives |
title_full |
Exploring the Photodynamic Properties of Two Antiproliferative Benzodiazopyrrole Derivatives |
title_fullStr |
Exploring the Photodynamic Properties of Two Antiproliferative Benzodiazopyrrole Derivatives |
title_full_unstemmed |
Exploring the Photodynamic Properties of Two Antiproliferative Benzodiazopyrrole Derivatives |
title_sort |
exploring the photodynamic properties of two antiproliferative benzodiazopyrrole derivatives |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2020-02-01 |
description |
The identification of molecules whose biological activity can be properly modulated by light is a promising therapeutic approach aimed to improve drug selectivity and efficacy on the molecular target and to limit the side effects compared to traditional drugs. Recently, two photo-switchable diastereomeric benzodiazopyrrole derivatives <b>1RR</b> and <b>1RS</b> have been reported as microtubules targeting agents (MTAs) on human colorectal carcinoma p53 null cell line (HCT 116 p53-/-). Their IC<sub>50</sub> was enhanced upon Light Emitting Diode (LED) irradiation at 435 nm and was related to their <i>cis</i> form. Here we have investigated the photo-responsive behavior of the acid derivatives of <b>1RR</b> and <b>1RS</b>, namely, <i>d</i><b>1RR</b> and <i>d</i><b>1RS</b>, in phosphate buffer solutions at different pH. The comparison of the UV spectra, acquired before and after LED irradiation, indicated that the <i>trans</i><i>→</i><i>cis</i> conversion of <i>d</i><b>1RR</b> and <i>d</i><b>1RS</b> is affected by the degree of ionization. The apparent rate constants were calculated from the kinetic data by means of fast UV spectroscopy and the conformers of the putative ionic species present in solution (pH range: 5.7−8.0) were modelled. Taken together, our experimental and theoretical results suggest that the photo-conversions of <i>trans</i> <i>d</i><b>1RR</b>/<i>d</i><b>1RS</b> into the corresponding <i>cis</i> forms and the thermal decay of <i>cis</i> <i>d</i><b>1RR</b>/<i>d</i><b>1RS</b> are dependent on the presence of diazonium form of <i>d</i><b>1RR</b>/<i>d</i><b>1RS</b>. Finally, a photo-reaction was detected only for <i>d</i><b>1RR</b> after prolonged LED irradiation in acidic medium, and the resulting product was characterized by means of Liquid Chromatography coupled to High resolution Mass Spectrometry (LC-HRMS) and Nuclear Magnetic Resonance (NMR) spectroscopy. |
topic |
photoswitchable azoheteroarene diazo derivative <i>cis</i>-<i>trans</i> conversion fast uv spectroscopy lc-hrms conformational analysis dft optimization |
url |
https://www.mdpi.com/1422-0067/21/4/1246 |
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