Extrajunctional resting Ca2+ influx is not increased in a severely dystrophic expiratory muscle (triangularis sterni) of the mdx mouse
Freshly isolated adult mdx and nondystrophic (C57B110SnJ) muscle fibers were used to examine the potential role of resting Ca2+ influx in the pathogenesis of Duchenne and related dystrophies. Microfluorimetric determinations of resting divalent cation influx were obtained from undissociated intact m...
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doaj-b92b84fc2ad84b4ca133b7a7fd813ce52021-03-20T04:48:42ZengElsevierNeurobiology of Disease1095-953X2003-11-01142229239Extrajunctional resting Ca2+ influx is not increased in a severely dystrophic expiratory muscle (triangularis sterni) of the mdx mouseC.George Carlson0Anton Gueorguiev1Diana M Roshek2Rebecca Ashmore3Jacquelyne S Chu4Judy E Anderson5Department of Physiology, Kirksville College of Osteopathic Medicine, Kirksville, MO 63501, USA; Department of Human Anatomy and Cell Science, University of Manitoba, 730 William Ave., Winnipeg, Manitoba R3E OW3, CanadaDepartment of Physiology, Kirksville College of Osteopathic Medicine, Kirksville, MO 63501, USA; Department of Human Anatomy and Cell Science, University of Manitoba, 730 William Ave., Winnipeg, Manitoba R3E OW3, CanadaDepartment of Physiology, Kirksville College of Osteopathic Medicine, Kirksville, MO 63501, USA; Department of Human Anatomy and Cell Science, University of Manitoba, 730 William Ave., Winnipeg, Manitoba R3E OW3, CanadaDepartment of Physiology, Kirksville College of Osteopathic Medicine, Kirksville, MO 63501, USA; Department of Human Anatomy and Cell Science, University of Manitoba, 730 William Ave., Winnipeg, Manitoba R3E OW3, CanadaDepartment of Physiology, Kirksville College of Osteopathic Medicine, Kirksville, MO 63501, USA; Department of Human Anatomy and Cell Science, University of Manitoba, 730 William Ave., Winnipeg, Manitoba R3E OW3, CanadaDepartment of Physiology, Kirksville College of Osteopathic Medicine, Kirksville, MO 63501, USA; Department of Human Anatomy and Cell Science, University of Manitoba, 730 William Ave., Winnipeg, Manitoba R3E OW3, CanadaFreshly isolated adult mdx and nondystrophic (C57B110SnJ) muscle fibers were used to examine the potential role of resting Ca2+ influx in the pathogenesis of Duchenne and related dystrophies. Microfluorimetric determinations of resting divalent cation influx were obtained from undissociated intact muscle fibers in the triangularis sterni (TS), a thin expiratory muscle. Morphological evidence indicated severe dystrophic alterations in the mdx TS at 5 months, and a prononunced loss of fibers with connective tissue infiltration in older animals. To examine resting Ca2+ influx, fibers were loaded with FURA PE3 and the rate of quenching of intracellular signal following the extracellular addition of Mn2+ was determined from extrajunctional regions. There was no significant difference in quench rate between nondystrophic and mdx TS fibers. These results indicate that severe dystrophic pathology in the absence of dystrophin is not due to generalized increases in resting Ca2+ influx.http://www.sciencedirect.com/science/article/pii/S0969996103001281Duchenne muscular dystrophymdx mouseDystrophinopathiesCalciumCalcium channelsMuscle necrosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
C.George Carlson Anton Gueorguiev Diana M Roshek Rebecca Ashmore Jacquelyne S Chu Judy E Anderson |
spellingShingle |
C.George Carlson Anton Gueorguiev Diana M Roshek Rebecca Ashmore Jacquelyne S Chu Judy E Anderson Extrajunctional resting Ca2+ influx is not increased in a severely dystrophic expiratory muscle (triangularis sterni) of the mdx mouse Neurobiology of Disease Duchenne muscular dystrophy mdx mouse Dystrophinopathies Calcium Calcium channels Muscle necrosis |
author_facet |
C.George Carlson Anton Gueorguiev Diana M Roshek Rebecca Ashmore Jacquelyne S Chu Judy E Anderson |
author_sort |
C.George Carlson |
title |
Extrajunctional resting Ca2+ influx is not increased in a severely dystrophic expiratory muscle (triangularis sterni) of the mdx mouse |
title_short |
Extrajunctional resting Ca2+ influx is not increased in a severely dystrophic expiratory muscle (triangularis sterni) of the mdx mouse |
title_full |
Extrajunctional resting Ca2+ influx is not increased in a severely dystrophic expiratory muscle (triangularis sterni) of the mdx mouse |
title_fullStr |
Extrajunctional resting Ca2+ influx is not increased in a severely dystrophic expiratory muscle (triangularis sterni) of the mdx mouse |
title_full_unstemmed |
Extrajunctional resting Ca2+ influx is not increased in a severely dystrophic expiratory muscle (triangularis sterni) of the mdx mouse |
title_sort |
extrajunctional resting ca2+ influx is not increased in a severely dystrophic expiratory muscle (triangularis sterni) of the mdx mouse |
publisher |
Elsevier |
series |
Neurobiology of Disease |
issn |
1095-953X |
publishDate |
2003-11-01 |
description |
Freshly isolated adult mdx and nondystrophic (C57B110SnJ) muscle fibers were used to examine the potential role of resting Ca2+ influx in the pathogenesis of Duchenne and related dystrophies. Microfluorimetric determinations of resting divalent cation influx were obtained from undissociated intact muscle fibers in the triangularis sterni (TS), a thin expiratory muscle. Morphological evidence indicated severe dystrophic alterations in the mdx TS at 5 months, and a prononunced loss of fibers with connective tissue infiltration in older animals. To examine resting Ca2+ influx, fibers were loaded with FURA PE3 and the rate of quenching of intracellular signal following the extracellular addition of Mn2+ was determined from extrajunctional regions. There was no significant difference in quench rate between nondystrophic and mdx TS fibers. These results indicate that severe dystrophic pathology in the absence of dystrophin is not due to generalized increases in resting Ca2+ influx. |
topic |
Duchenne muscular dystrophy mdx mouse Dystrophinopathies Calcium Calcium channels Muscle necrosis |
url |
http://www.sciencedirect.com/science/article/pii/S0969996103001281 |
work_keys_str_mv |
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