The follicle epithelium in the Drosophila ovary is maintained by a small number of stem cells
The follicle stem cells (FSCs) in the Drosophila ovary are an important experimental model for the study of epithelial stem cell biology. Although decades of research support the conclusion that there are two FSCs per ovariole, a recent study used a novel clonal marking system to conclude that there...
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doaj-b92e6c7d43e546ef81ce20e91c7ede382021-05-05T18:11:48ZengeLife Sciences Publications LtdeLife2050-084X2019-12-01810.7554/eLife.49050The follicle epithelium in the Drosophila ovary is maintained by a small number of stem cellsJocelyne Fadiga0Todd G Nystul1https://orcid.org/0000-0002-6250-2394Department of Anatomy, University of California, San Francisco, San Francisco, United States; Department of OB/GYN-RS, Center for Reproductive Sciences, University of California, San Francisco, San Francisco, United StatesDepartment of Anatomy, University of California, San Francisco, San Francisco, United States; Department of OB/GYN-RS, Center for Reproductive Sciences, University of California, San Francisco, San Francisco, United StatesThe follicle stem cells (FSCs) in the Drosophila ovary are an important experimental model for the study of epithelial stem cell biology. Although decades of research support the conclusion that there are two FSCs per ovariole, a recent study used a novel clonal marking system to conclude that there are 15–16 FSCs per ovariole. We performed clonal analysis using both this novel clonal marking system and standard clonal marking systems, and identified several problems that may have contributed to the overestimate of FSC number. In addition, we developed new methods for accurately measuring clone size, and found that FSC clones produce, on average, half of the follicle cells in each ovariole. Our findings provide strong independent support for the conclusion that there are typically two active FSCs per ovariole, though they are consistent with up to four FSCs per germarium.https://elifesciences.org/articles/49050epithelial stem cellsovaryclonal analysisfollicle cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jocelyne Fadiga Todd G Nystul |
spellingShingle |
Jocelyne Fadiga Todd G Nystul The follicle epithelium in the Drosophila ovary is maintained by a small number of stem cells eLife epithelial stem cells ovary clonal analysis follicle cells |
author_facet |
Jocelyne Fadiga Todd G Nystul |
author_sort |
Jocelyne Fadiga |
title |
The follicle epithelium in the Drosophila ovary is maintained by a small number of stem cells |
title_short |
The follicle epithelium in the Drosophila ovary is maintained by a small number of stem cells |
title_full |
The follicle epithelium in the Drosophila ovary is maintained by a small number of stem cells |
title_fullStr |
The follicle epithelium in the Drosophila ovary is maintained by a small number of stem cells |
title_full_unstemmed |
The follicle epithelium in the Drosophila ovary is maintained by a small number of stem cells |
title_sort |
follicle epithelium in the drosophila ovary is maintained by a small number of stem cells |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2019-12-01 |
description |
The follicle stem cells (FSCs) in the Drosophila ovary are an important experimental model for the study of epithelial stem cell biology. Although decades of research support the conclusion that there are two FSCs per ovariole, a recent study used a novel clonal marking system to conclude that there are 15–16 FSCs per ovariole. We performed clonal analysis using both this novel clonal marking system and standard clonal marking systems, and identified several problems that may have contributed to the overestimate of FSC number. In addition, we developed new methods for accurately measuring clone size, and found that FSC clones produce, on average, half of the follicle cells in each ovariole. Our findings provide strong independent support for the conclusion that there are typically two active FSCs per ovariole, though they are consistent with up to four FSCs per germarium. |
topic |
epithelial stem cells ovary clonal analysis follicle cells |
url |
https://elifesciences.org/articles/49050 |
work_keys_str_mv |
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1721458776408588288 |