Astragaloside IV Enhances Cisplatin Chemosensitivity in Non-Small Cell Lung Cancer Cells Through Inhibition of B7-H3
Background: Chemoresistance is a major obstacle to successful chemotherapy for human non-small cell lung cancer (NSCLC). Astragaloside IV, the component of Astragalus membranaceus, has been reported to exhibit anti-inflammation, anti-cancer and immunoregulatory properties. In the present study, we i...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Cell Physiol Biochem Press GmbH & Co KG
2016-12-01
|
Series: | Cellular Physiology and Biochemistry |
Subjects: | |
Online Access: | http://www.karger.com/Article/FullText/453175 |
id |
doaj-b933af18e84b4a5e84f4a8f5fbcdfcf9 |
---|---|
record_format |
Article |
spelling |
doaj-b933af18e84b4a5e84f4a8f5fbcdfcf92020-11-25T02:46:52ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782016-12-014051221122910.1159/000453175453175Astragaloside IV Enhances Cisplatin Chemosensitivity in Non-Small Cell Lung Cancer Cells Through Inhibition of B7-H3Cheng-Shi HeYi-Cheng LiuZhi-Peng XuPeng-Chen DaiXiao-Wei ChenDe-Hai JinBackground: Chemoresistance is a major obstacle to successful chemotherapy for human non-small cell lung cancer (NSCLC). Astragaloside IV, the component of Astragalus membranaceus, has been reported to exhibit anti-inflammation, anti-cancer and immunoregulatory properties. In the present study, we investigated the role of astragaloside IV in the chemoresistance to cisplatin in NSCLC cells. Methods: We established astragaloside IV-suppressed NSCLC cell lines including A549, HCC827, and NCI-H1299 and evaluated their sensitivity to cisplatin in vitro. In addition, we examined the mRNA and protein levels of B7-H3 in response to cisplatin-based chemotherapy. Results: We showed that high doses of astragaloside IV (10, 20, 40 ng/ml) inhibited NSCLC cell growth, whereas low concentrations of astragaloside IV (1, 2.5, 5 ng/ml) had no obvious cytotoxicity on cell viability. Moreover, combined treatment with astragaloside IV significantly increased chemosensitivity to cisplatin in NSCLC cells. On the molecular level, astragaloside IV co-treatment significantly inhibited the mRNA and protein levels of B7-H3 in the presence of cisplatin. In addition, ectopic expression of B7-H3 diminished the sensitization role of astragaloside IV in cellular responses to cisplatin in NSCLC cells. Conclusion: These results demonstrate that astragaloside IV enhances chemosensitivity to cisplatin via inhibition of B7-H3 and that treatment with astragaloside IV and inhibition of B7-H3 serve as potential therapeutic approach for lung cancer patients.http://www.karger.com/Article/FullText/453175ChemoresistanceAstragaloside IVB7-H3Non-small cell lung cancer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cheng-Shi He Yi-Cheng Liu Zhi-Peng Xu Peng-Chen Dai Xiao-Wei Chen De-Hai Jin |
spellingShingle |
Cheng-Shi He Yi-Cheng Liu Zhi-Peng Xu Peng-Chen Dai Xiao-Wei Chen De-Hai Jin Astragaloside IV Enhances Cisplatin Chemosensitivity in Non-Small Cell Lung Cancer Cells Through Inhibition of B7-H3 Cellular Physiology and Biochemistry Chemoresistance Astragaloside IV B7-H3 Non-small cell lung cancer |
author_facet |
Cheng-Shi He Yi-Cheng Liu Zhi-Peng Xu Peng-Chen Dai Xiao-Wei Chen De-Hai Jin |
author_sort |
Cheng-Shi He |
title |
Astragaloside IV Enhances Cisplatin Chemosensitivity in Non-Small Cell Lung Cancer Cells Through Inhibition of B7-H3 |
title_short |
Astragaloside IV Enhances Cisplatin Chemosensitivity in Non-Small Cell Lung Cancer Cells Through Inhibition of B7-H3 |
title_full |
Astragaloside IV Enhances Cisplatin Chemosensitivity in Non-Small Cell Lung Cancer Cells Through Inhibition of B7-H3 |
title_fullStr |
Astragaloside IV Enhances Cisplatin Chemosensitivity in Non-Small Cell Lung Cancer Cells Through Inhibition of B7-H3 |
title_full_unstemmed |
Astragaloside IV Enhances Cisplatin Chemosensitivity in Non-Small Cell Lung Cancer Cells Through Inhibition of B7-H3 |
title_sort |
astragaloside iv enhances cisplatin chemosensitivity in non-small cell lung cancer cells through inhibition of b7-h3 |
publisher |
Cell Physiol Biochem Press GmbH & Co KG |
series |
Cellular Physiology and Biochemistry |
issn |
1015-8987 1421-9778 |
publishDate |
2016-12-01 |
description |
Background: Chemoresistance is a major obstacle to successful chemotherapy for human non-small cell lung cancer (NSCLC). Astragaloside IV, the component of Astragalus membranaceus, has been reported to exhibit anti-inflammation, anti-cancer and immunoregulatory properties. In the present study, we investigated the role of astragaloside IV in the chemoresistance to cisplatin in NSCLC cells. Methods: We established astragaloside IV-suppressed NSCLC cell lines including A549, HCC827, and NCI-H1299 and evaluated their sensitivity to cisplatin in vitro. In addition, we examined the mRNA and protein levels of B7-H3 in response to cisplatin-based chemotherapy. Results: We showed that high doses of astragaloside IV (10, 20, 40 ng/ml) inhibited NSCLC cell growth, whereas low concentrations of astragaloside IV (1, 2.5, 5 ng/ml) had no obvious cytotoxicity on cell viability. Moreover, combined treatment with astragaloside IV significantly increased chemosensitivity to cisplatin in NSCLC cells. On the molecular level, astragaloside IV co-treatment significantly inhibited the mRNA and protein levels of B7-H3 in the presence of cisplatin. In addition, ectopic expression of B7-H3 diminished the sensitization role of astragaloside IV in cellular responses to cisplatin in NSCLC cells. Conclusion: These results demonstrate that astragaloside IV enhances chemosensitivity to cisplatin via inhibition of B7-H3 and that treatment with astragaloside IV and inhibition of B7-H3 serve as potential therapeutic approach for lung cancer patients. |
topic |
Chemoresistance Astragaloside IV B7-H3 Non-small cell lung cancer |
url |
http://www.karger.com/Article/FullText/453175 |
work_keys_str_mv |
AT chengshihe astragalosideivenhancescisplatinchemosensitivityinnonsmallcelllungcancercellsthroughinhibitionofb7h3 AT yichengliu astragalosideivenhancescisplatinchemosensitivityinnonsmallcelllungcancercellsthroughinhibitionofb7h3 AT zhipengxu astragalosideivenhancescisplatinchemosensitivityinnonsmallcelllungcancercellsthroughinhibitionofb7h3 AT pengchendai astragalosideivenhancescisplatinchemosensitivityinnonsmallcelllungcancercellsthroughinhibitionofb7h3 AT xiaoweichen astragalosideivenhancescisplatinchemosensitivityinnonsmallcelllungcancercellsthroughinhibitionofb7h3 AT dehaijin astragalosideivenhancescisplatinchemosensitivityinnonsmallcelllungcancercellsthroughinhibitionofb7h3 |
_version_ |
1724756252086501376 |