Rooibos (Aspalathus linearis) extract, containing eriodictyol-6-C-β-d-glucoside as the active component, stimulates exocrine glands via the M3 muscarinic acetylcholine receptor
We previously discovered that rooibos extract activates the M3 muscarinic acetylcholine receptor (M3R), a key component of exocrine secretion. In this study, we examined the rooibos extract for active components in vitro and monitored its fast-acting effects against dryness in humans. Our in vitro a...
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doaj-b9537791bf434efcb6e153229663a98e2021-04-30T07:15:49ZengElsevierJournal of Functional Foods1756-46462019-04-0155248254Rooibos (Aspalathus linearis) extract, containing eriodictyol-6-C-β-d-glucoside as the active component, stimulates exocrine glands via the M3 muscarinic acetylcholine receptorSaori Nishimachi0Takao Iwai1Yoshitaka Nakamura2Kanako Yuyama-Masuda3Takuya Tsutsui4Noriyuki Monoi5Akira Uchiyama6Seiji Nakamura7Research & Development Headquarters, Lion Corporation, JapanResearch & Development Headquarters, Lion Corporation, JapanResearch & Development Headquarters, Lion Corporation, JapanResearch & Development Headquarters, Lion Corporation, JapanResearch & Development Headquarters, Lion Corporation, JapanResearch & Development Headquarters, Lion Corporation, Japan; Corresponding author at: Wellness Research Laboratories, Research & Development Headquarters, Lion Corporation, 100 Tajima, Odawara, Kanagawa Prefecture 256-0811, Japan.Research & Development Headquarters, Lion Corporation, Japan; Division of Oral Health, Growth, and Development, Faculty of Dental Science, Kyushu University, JapanSection of Oral and Maxillofacial Oncology, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, JapanWe previously discovered that rooibos extract activates the M3 muscarinic acetylcholine receptor (M3R), a key component of exocrine secretion. In this study, we examined the rooibos extract for active components in vitro and monitored its fast-acting effects against dryness in humans. Our in vitro analysis demonstrated that the M3R-stimulating effect of rooibos extract was similar to that of eriodictyol-6-C-β-d-glucoside (E6CG), whereas other rooibos constituents (aspalathin, orientin, isoorientin or eriodictyol-8-C-β-d-glucoside) were not active. In a placebo-controlled, double-blinded crossover clinical trial, 36 subjects with dryness at the mouth, eyes, and skin consumed a daily dose of either 100 mg rooibos or placebo tablets for three days. Rooibos extract ingestion increased resting-saliva content, tear volume, and stratum corneum moisture content, and reduced the dryness symptoms. In conclusion, rooibos extract contains E6CG as an M3R activator and stimulates exocrine gland secretion, which relieves dryness at the mouth, eyes, and skin in humans.http://www.sciencedirect.com/science/article/pii/S1756464619300805TeaSalivaTearStratum corneum moisture contentDrynessFlavonoid |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Saori Nishimachi Takao Iwai Yoshitaka Nakamura Kanako Yuyama-Masuda Takuya Tsutsui Noriyuki Monoi Akira Uchiyama Seiji Nakamura |
spellingShingle |
Saori Nishimachi Takao Iwai Yoshitaka Nakamura Kanako Yuyama-Masuda Takuya Tsutsui Noriyuki Monoi Akira Uchiyama Seiji Nakamura Rooibos (Aspalathus linearis) extract, containing eriodictyol-6-C-β-d-glucoside as the active component, stimulates exocrine glands via the M3 muscarinic acetylcholine receptor Journal of Functional Foods Tea Saliva Tear Stratum corneum moisture content Dryness Flavonoid |
author_facet |
Saori Nishimachi Takao Iwai Yoshitaka Nakamura Kanako Yuyama-Masuda Takuya Tsutsui Noriyuki Monoi Akira Uchiyama Seiji Nakamura |
author_sort |
Saori Nishimachi |
title |
Rooibos (Aspalathus linearis) extract, containing eriodictyol-6-C-β-d-glucoside as the active component, stimulates exocrine glands via the M3 muscarinic acetylcholine receptor |
title_short |
Rooibos (Aspalathus linearis) extract, containing eriodictyol-6-C-β-d-glucoside as the active component, stimulates exocrine glands via the M3 muscarinic acetylcholine receptor |
title_full |
Rooibos (Aspalathus linearis) extract, containing eriodictyol-6-C-β-d-glucoside as the active component, stimulates exocrine glands via the M3 muscarinic acetylcholine receptor |
title_fullStr |
Rooibos (Aspalathus linearis) extract, containing eriodictyol-6-C-β-d-glucoside as the active component, stimulates exocrine glands via the M3 muscarinic acetylcholine receptor |
title_full_unstemmed |
Rooibos (Aspalathus linearis) extract, containing eriodictyol-6-C-β-d-glucoside as the active component, stimulates exocrine glands via the M3 muscarinic acetylcholine receptor |
title_sort |
rooibos (aspalathus linearis) extract, containing eriodictyol-6-c-β-d-glucoside as the active component, stimulates exocrine glands via the m3 muscarinic acetylcholine receptor |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2019-04-01 |
description |
We previously discovered that rooibos extract activates the M3 muscarinic acetylcholine receptor (M3R), a key component of exocrine secretion. In this study, we examined the rooibos extract for active components in vitro and monitored its fast-acting effects against dryness in humans. Our in vitro analysis demonstrated that the M3R-stimulating effect of rooibos extract was similar to that of eriodictyol-6-C-β-d-glucoside (E6CG), whereas other rooibos constituents (aspalathin, orientin, isoorientin or eriodictyol-8-C-β-d-glucoside) were not active. In a placebo-controlled, double-blinded crossover clinical trial, 36 subjects with dryness at the mouth, eyes, and skin consumed a daily dose of either 100 mg rooibos or placebo tablets for three days. Rooibos extract ingestion increased resting-saliva content, tear volume, and stratum corneum moisture content, and reduced the dryness symptoms. In conclusion, rooibos extract contains E6CG as an M3R activator and stimulates exocrine gland secretion, which relieves dryness at the mouth, eyes, and skin in humans. |
topic |
Tea Saliva Tear Stratum corneum moisture content Dryness Flavonoid |
url |
http://www.sciencedirect.com/science/article/pii/S1756464619300805 |
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