Smoking cessation and bronchial epithelial remodelling in COPD: a cross-sectional study
<p>Abstract</p> <p>Background</p> <p>Chronic Obstructive Pulmonary Disease (COPD) is associated with bronchial epithelial changes, including squamous cell metaplasia and goblet cell hyperplasia. These features are partially attributed to activation of the epidermal grow...
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doaj-b953dd38475441f4866b989157d235172020-11-24T20:53:39ZengBMCRespiratory Research1465-99212007-11-01818510.1186/1465-9921-8-85Smoking cessation and bronchial epithelial remodelling in COPD: a cross-sectional studyBajema Ingeborg MPostma Dirkje SGosman Margot MEvan Schadewijk AnnemarieSont Jacob KLapperre Thérèse STimens WimMauad ThaisHiemstra Pieter S<p>Abstract</p> <p>Background</p> <p>Chronic Obstructive Pulmonary Disease (COPD) is associated with bronchial epithelial changes, including squamous cell metaplasia and goblet cell hyperplasia. These features are partially attributed to activation of the epidermal growth factor receptor (EGFR). Whereas smoking cessation reduces respiratory symptoms and lung function decline in COPD, inflammation persists. We determined epithelial proliferation and composition in bronchial biopsies from current and ex-smokers with COPD, and its relation to duration of smoking cessation.</p> <p>Methods</p> <p>114 COPD patients were studied cross-sectionally: 99 males/15 females, age 62 ± 8 years, median 42 pack-years, no corticosteroids, current (n = 72) or ex-smokers (n = 42, median cessation duration 3.5 years), postbronchodilator FEV<sub>1 </sub>63 ± 9% predicted. Squamous cell metaplasia (%), goblet cell (PAS/Alcian Blue<sup>+</sup>) area (%), proliferating (Ki-67<sup>+</sup>) cell numbers (/mm basement membrane), and EGFR expression (%) were measured in intact epithelium of bronchial biopsies.</p> <p>Results</p> <p>Ex-smokers with COPD had significantly less epithelial squamous cell metaplasia, proliferating cell numbers, and a trend towards reduced goblet cell area than current smokers with COPD (p = 0.025, p = 0.001, p = 0.081, respectively), but no significant difference in EGFR expression. Epithelial features were not different between short-term quitters (<3.5 years) and current smokers. Long-term quitters (≥3.5 years) had less goblet cell area than both current smokers and short-term quitters (medians: 7.9% vs. 14.4%, p = 0.005; 7.9% vs. 13.5%, p = 0.008; respectively), and less proliferating cell numbers than current smokers (2.8% vs. 18.6%, p < 0.001).</p> <p>Conclusion</p> <p>Ex-smokers with COPD had less bronchial epithelial remodelling than current smokers, which was only observed after long-term smoking cessation (>3.5 years).</p> <p>Trial registration</p> <p>NCT00158847</p> http://respiratory-research.com/content/8/1/85 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bajema Ingeborg M Postma Dirkje S Gosman Margot ME van Schadewijk Annemarie Sont Jacob K Lapperre Thérèse S Timens Wim Mauad Thais Hiemstra Pieter S |
spellingShingle |
Bajema Ingeborg M Postma Dirkje S Gosman Margot ME van Schadewijk Annemarie Sont Jacob K Lapperre Thérèse S Timens Wim Mauad Thais Hiemstra Pieter S Smoking cessation and bronchial epithelial remodelling in COPD: a cross-sectional study Respiratory Research |
author_facet |
Bajema Ingeborg M Postma Dirkje S Gosman Margot ME van Schadewijk Annemarie Sont Jacob K Lapperre Thérèse S Timens Wim Mauad Thais Hiemstra Pieter S |
author_sort |
Bajema Ingeborg M |
title |
Smoking cessation and bronchial epithelial remodelling in COPD: a cross-sectional study |
title_short |
Smoking cessation and bronchial epithelial remodelling in COPD: a cross-sectional study |
title_full |
Smoking cessation and bronchial epithelial remodelling in COPD: a cross-sectional study |
title_fullStr |
Smoking cessation and bronchial epithelial remodelling in COPD: a cross-sectional study |
title_full_unstemmed |
Smoking cessation and bronchial epithelial remodelling in COPD: a cross-sectional study |
title_sort |
smoking cessation and bronchial epithelial remodelling in copd: a cross-sectional study |
publisher |
BMC |
series |
Respiratory Research |
issn |
1465-9921 |
publishDate |
2007-11-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Chronic Obstructive Pulmonary Disease (COPD) is associated with bronchial epithelial changes, including squamous cell metaplasia and goblet cell hyperplasia. These features are partially attributed to activation of the epidermal growth factor receptor (EGFR). Whereas smoking cessation reduces respiratory symptoms and lung function decline in COPD, inflammation persists. We determined epithelial proliferation and composition in bronchial biopsies from current and ex-smokers with COPD, and its relation to duration of smoking cessation.</p> <p>Methods</p> <p>114 COPD patients were studied cross-sectionally: 99 males/15 females, age 62 ± 8 years, median 42 pack-years, no corticosteroids, current (n = 72) or ex-smokers (n = 42, median cessation duration 3.5 years), postbronchodilator FEV<sub>1 </sub>63 ± 9% predicted. Squamous cell metaplasia (%), goblet cell (PAS/Alcian Blue<sup>+</sup>) area (%), proliferating (Ki-67<sup>+</sup>) cell numbers (/mm basement membrane), and EGFR expression (%) were measured in intact epithelium of bronchial biopsies.</p> <p>Results</p> <p>Ex-smokers with COPD had significantly less epithelial squamous cell metaplasia, proliferating cell numbers, and a trend towards reduced goblet cell area than current smokers with COPD (p = 0.025, p = 0.001, p = 0.081, respectively), but no significant difference in EGFR expression. Epithelial features were not different between short-term quitters (<3.5 years) and current smokers. Long-term quitters (≥3.5 years) had less goblet cell area than both current smokers and short-term quitters (medians: 7.9% vs. 14.4%, p = 0.005; 7.9% vs. 13.5%, p = 0.008; respectively), and less proliferating cell numbers than current smokers (2.8% vs. 18.6%, p < 0.001).</p> <p>Conclusion</p> <p>Ex-smokers with COPD had less bronchial epithelial remodelling than current smokers, which was only observed after long-term smoking cessation (>3.5 years).</p> <p>Trial registration</p> <p>NCT00158847</p> |
url |
http://respiratory-research.com/content/8/1/85 |
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